Navigation Links
High-Titer ,Retroviral Vectors for Gene Delivery

sfer Systems

Fig.1

Nonreplicating retroviral vectors contain all of the cis elements required for transcription of mRNA molecules encoding a gene of interest and packaging of these transcripts into infectious virus particles (Figure 1). The vectors typically comprise an E. coli plasmid backbone containing a pair of 600-bp viral long terminal repeats (LTRs) between which the gene of interest is inserted. The LTR is divided into three regions: The U3 region contains the retroviral promoter/enhancer, and is flanked in the 3 direction by the R region, which contains the viral polyadenylation signal (pA). The R region is followed by the U5 region, which, along with R, contains sequences that are critical for reverse transcription. Expression of the viral RNA is initiated within the U3 region of the 5 LTR and is terminated in the R region of the 3 LTR. The viral packaging signal (Y), which is required in cis for the viral RNA to be packaged into virion particles, resides between the 5 LTR and the coding sequence for the gene of interest.

To generate infectious virus particles that carry the gene of interest, specialized packaging cell lines have been constructed that contain chromosomally integrated expression cassettes for viral gag, pol, and env proteins, all of which are required in trans to make virus.2 The gag gene encodes internal structural proteins; the pol gene encodes reverse transcriptase (RT) and integrase; and the env gene encodes the viral envelope protein, which resides on the viral surface and facilitates infection of the target cell b
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8

Related biology technology :

1. Safe Production of High-Titer Retrovirus
2. Generate Adenovirus Vectors in E. coli by Homologous Recombination with the AdEasy Adenoviral Vector System
3. Versatile Vectors for Ponasterone A- Inducible Control of Gene Expression in Mammalian Cells
4. Epitope-Tagging Vectors for Functional Analysis in Yeast
5. New Mammalian Expression Vectors Employ Stable, High-Level Fluorescence Humanized Renilla GFP Reporter
6. Efficiently Insert Unique Restriction Sites into Plasmid Vectors
7. High-Efficiency Site-Directed Mutagenesis of Large Plasmid Vectors
8. Versatile Reporter Vectors for Monitoring Viral Transduction
9. Improved Vectors for PathDetect Trans-Reporting Systems
10. Mammalian Expression Vectors with Interchangeable Selectable Markers
11. Delivering siRNA Using Adenoviral Vectors
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: High Titer Retroviral Vectors for Gene Delivery

(Date:9/30/2014)... , Sept. 30, 2014 Pacific ... 2014 Summary Global Markets Direct,s, ,Pacific ... 2014, provides an overview of the Pacific Northwest ... This report provides comprehensive information on the current ... complete with comparative analysis at various stages, therapeutics ...
(Date:9/30/2014)... (PRWEB) September 30, 2014 Shimadzu ... extensometer, an easy-to-use and highly precise tool for ... with considerable elongation. With 1000-mm maximum movement distance, ... percent for a gauge length of 10 mm. ... measurement accuracy above 50-mm stroke and within +/- ...
(Date:9/30/2014)... (PRWEB) September 30, 2014 Over the ... primarily due to rising R&D budgets and increased acceptance ... fast growing pharmaceutical and biotechnology industries are also catalyzing ... laboratory filtration market is expected to grow at a ... is estimated to be worth $2,532.6 million by 2019. ...
(Date:9/30/2014)... 30, 2014 Follow us on ... each type of cholesterol carrier or lipoprotein in the ... excessive cholesterol levels in the body, especially along the ... heart attack and other cardiovascular disorders. Cardiovascular Diseases (CVD) ... for cholesterol levels on a regular basis, to assess ...
Breaking Biology Technology:Pacific Northwest Biotechnology, LLC - Product Pipeline Review - 2014 2Pacific Northwest Biotechnology, LLC - Product Pipeline Review - 2014 3Shimadzu’s New Long-Travel Extensometer Increases Precision for Soft Material Testing 2Global Laboratory Filtration Market To See 7.0% CAGR to 2019 Says a Research Report Available at ReportsnReports.com 2Global Laboratory Filtration Market To See 7.0% CAGR to 2019 Says a Research Report Available at ReportsnReports.com 3Global Laboratory Filtration Market To See 7.0% CAGR to 2019 Says a Research Report Available at ReportsnReports.com 4Global Laboratory Filtration Market To See 7.0% CAGR to 2019 Says a Research Report Available at ReportsnReports.com 5Surging Diabetic, Obese and Hypertensive Population Drives the Global Cholesterol Testing Market, According to New Report by Global Industry Analysts, Inc. 2Surging Diabetic, Obese and Hypertensive Population Drives the Global Cholesterol Testing Market, According to New Report by Global Industry Analysts, Inc. 3Surging Diabetic, Obese and Hypertensive Population Drives the Global Cholesterol Testing Market, According to New Report by Global Industry Analysts, Inc. 4
... Medicine Project of, Seattle (CHIMPS) Presented at Academic Pediatric Association Conference ... ... CHELMSFORD, Mass., April 22 El Salvador, like many,emerging markets, must ... public concern. This past fall, the El Salvadoran,government threatened to shut ...
... Comprise One of the Main User Groups of febit,s ... Simplified and Automated Microarray Technology, MEDFORD, Mass., and ... will introduce the Geniom RT Analyzer(R) to,the U.S. market at ... Boston., RNA-researchers, one of the main user groups of ...
... Netherlands and SANTA CLARA, Calif., April 22 /PRNewswire/,-- ... evolving field of molecular,diagnostics, and Agilent Technologies, Inc. ... they intend to collaborate to develop new in-vitro,diagnostic ... agreement in,which Agendia products will continue to be ...
Cached Biology Technology:ESA LeadCare(R) System Used to Identify Salvadoran Children with Lead Poisoning 2ESA LeadCare(R) System Used to Identify Salvadoran Children with Lead Poisoning 3ESA LeadCare(R) System Used to Identify Salvadoran Children with Lead Poisoning 4febit to Introduce Geniom RT Analyzer(R) to U.S. Market at RNAi World Congress 2febit to Introduce Geniom RT Analyzer(R) to U.S. Market at RNAi World Congress 3Agendia and Agilent Announce Plans to Jointly Develop New Diagnostic Tests, Extend Supply Agreement 2Agendia and Agilent Announce Plans to Jointly Develop New Diagnostic Tests, Extend Supply Agreement 3
(Date:9/30/2014)... goes wrong with your muscles because of age, disease or ... is. That,s where a new research report published in the ... comes in. In the report, a team of scientists ... help scientists "see" which genes are active in an organ ... activity and that men have approximately 400 more active genes ...
(Date:9/30/2014)... perilous threats in today,s ocean. From overfishing and ... coral ecosystems are disappearing at unprecedented rates around ... corals surrounding the island of Moorea in French ... environment: coral guard-crabs. New research from the National ... Seabird McKeon and the museum,s predoctoral fellow Jenna ...
(Date:9/29/2014)... new study confirms that the exposure to tar tended ... of regular cigarettes. Similarly, exposure to nicotine tended ... popular type of cigarette in several countries around the ... certain toxic chemicals in the smoke of these cigarettes ... lower levels of chemicals in the smoke are not ...
Breaking Biology News(10 mins):Scientists identify which genes are active in muscles of men and women 2Smithsonian scientists discover coral's best defender against an army of sea stars 2Slim cigarette smokers not exposed to more harmful chemicals 2
... function of a newly discovered gene in corn may ... reducing childhood blindness and mortality rates, according to a ... Chair of Translational Genomics and professor of agronomy at ... and particularly orange corn, a type he said likely ...
... of Michigan and Shanghai Jiao Tong University in ... renewable energy and biomedical research projects involving investigators ... renewable-energy program is to develop new technologies that ... climate change. The biomedical collaboration will spur technological ...
... This release is available in French . ... have genetically manipulated rats that can emulate Alzheimer,s disease in ... treatments. Alzheimer,s is a devastating brain condition leading to a ... research mice have been developed in the past, rats are ...
Cached Biology News:Orange corn holds promise for reducing blindness, child death 2U-M joins with Chinese university to jointly fund renewable energy and biomedical research projects 2
Polyclonal Antibody to SHPRH...
Rabbit polyclonal to TetR ( Abpromise for all tested applications). Antigen: Synthetic peptide: AQKLGVEQPTLYWHVKNKR, corresponding to amino acids 31 - 49 of TetR...
Biotin anti-mouse Ly-49C/F/I/H...
Peptide-affinity Purified Polyclonal Antibody to Retinoic Acid-Induced 3 (RAI3/RAIG1)...
Biology Products: