Navigation Links
High-Titer ,Retroviral Vectors for Gene Delivery

by varying the multiplicity of infection, in contrast to standard transfection methods for which typically a small population of transfected cells are capable of the uptake and stable integration of vector DNA and for which the copy number is unpredicatable and often prohibitively high for many applications.3 The combination of high transduction efficiency and copy number control make retroviral delivery systems particularly useful for mammalian cDNA expression library production and screening. In these types of experiments, transduction of a large number of target cells with single-copy cDNA expression cassettes is highly desirable.4 The ability to efficiently transduce a wide range of hard-to-transfect cell types has also facilitated the reconstruction of transgenic animals. Additionally, retroviral vectors have become the system of choice for the in vivo delivery of therapeutic genes in the clinic.

We introduce the retroviral vectors pFB and pFB-Neo, which are based on MMLV and contain an extended packaging sequence and splice-site configuration that increases viral titer and gene expression from the viral promoter.5 These vectors can be used with any MMLV-based packaging cell line. MMLV-based virions are labile and readily inactivated by ethanol, ultraviolet light, and human complement.6 Thus, virus produced with these vectors, using packaging cell lines that allow infection of human cells (e.g., amphotropic packaging cells), are relatively safe and can be used in a P2 tissue culture facility.

We have achieved titers in excess of 108 cfu/ml with pFB and greater than 107 cfu/ml with pFB-Neo using an amphotropic packaging cell line; we found that expression levels for these vectors were generally comparable to or higher than those for other commercially available retroviral vectors.

Replication-Defective Retroviral Gene Tran
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8

Related biology technology :

1. Safe Production of High-Titer Retrovirus
2. Generate Adenovirus Vectors in E. coli by Homologous Recombination with the AdEasy Adenoviral Vector System
3. Versatile Vectors for Ponasterone A- Inducible Control of Gene Expression in Mammalian Cells
4. Epitope-Tagging Vectors for Functional Analysis in Yeast
5. New Mammalian Expression Vectors Employ Stable, High-Level Fluorescence Humanized Renilla GFP Reporter
6. Efficiently Insert Unique Restriction Sites into Plasmid Vectors
7. High-Efficiency Site-Directed Mutagenesis of Large Plasmid Vectors
8. Versatile Reporter Vectors for Monitoring Viral Transduction
9. Improved Vectors for PathDetect Trans-Reporting Systems
10. Mammalian Expression Vectors with Interchangeable Selectable Markers
11. Delivering siRNA Using Adenoviral Vectors

Post Your Comments:
*Name:
*Comment:
*Email:
TAG: High Titer Retroviral Vectors for Gene Delivery

(Date:4/30/2015)... 2015 WuXi PharmaTech (Cayman) Inc. ("WuXi" or ... contract R&D services provider, today announced that its board ... dated April 29, 2015, from a consortium (the "Consortium") ... founder, chairman and chief executive officer of the Company, ... a transaction (the "Transaction") involving the acquisition of all ...
(Date:4/30/2015)... SureClinical, a leader in cloud-based health science ... SureClinical’s executive team as Chief Financial Officer. ... with over 25 years in leadership roles ranging from ... Morris joins SureClinical from Marrone Bio Innovations (MBI), a ... MBI from its founding in 2006 until 2011, when ...
(Date:4/29/2015)... 30, 2015 According to a ... by Product (Portable & Non-Portable), Component (Accelerometer, Proximity ... Chemical, Metal & Mining, & Others), & Geography ... is expected to reach $1,453.76 Million by 2020, ... market data tables and 92 figures spread through ...
(Date:4/29/2015)... 2015   Doximity , the leading online medical ... as members, announced a new, instant method for physicians ... With one click, all US physicians can quickly submit ... for an open job opportunity, and a recruiter will ... seven percent of physicians are open to new jobs, ...
Breaking Biology Technology:WuXi PharmaTech Announces Receipt of Proposal 2WuXi PharmaTech Announces Receipt of Proposal 3WuXi PharmaTech Announces Receipt of Proposal 4WuXi PharmaTech Announces Receipt of Proposal 5WuXi PharmaTech Announces Receipt of Proposal 6Julie I. Morris Joins SureClinical as Chief Financial Officer 2Vibration Monitoring Equipment (Portable & Non-Portable) Market worth $1,453.76 Million by 2020 - New Report by MarketsandMarkets 2Vibration Monitoring Equipment (Portable & Non-Portable) Market worth $1,453.76 Million by 2020 - New Report by MarketsandMarkets 3Vibration Monitoring Equipment (Portable & Non-Portable) Market worth $1,453.76 Million by 2020 - New Report by MarketsandMarkets 4Doximity Offering Hospitals Easy, Efficient Physician Application Tool 2
... Wash.The world,s biggest Roman candle has got nothing on ... in the Earth or on a giant planet, Washington ... capable of storing vast amounts of energy. "If you ... of energy storage outside of nuclear energy," says Choong-Shik ...
... engineering school in Grenoble, France, and Virginia Tech is ... The students, work will help gene synthesis companies and ... DNA as harmful agents for bioterrorism. Synthetic biology ... production, agriculture, and other areas. For a few thousand ...
... Nanoscience has garnered billions of dollars of funding and ... Revolution. But, for such a richly anticipated field, ... us from odor-eating socks to cosmetics and medications ... with visions of nanotechnology as cornucopia or Armageddon. ...
Cached Biology Technology:iGEM team helps prevent rogue use of synthetic biology 2New resource examines questions about the role of nanoscience 2
(Date:4/14/2015)...  HYPR Corp. today announced it has joined ... tm , an industry consortium transforming online authentication ... technology and collaborate to deliver open specifications for ... and private, and easier to use. ... sensitive user information and eliminates the dependency on ...
(Date:4/10/2015)... 10, 2015 Research and Markets ... "Security Competitive Profiles - NEC" report to ... NEC will continue to supply a range of ... with a company focus on the development of a ... Winning opportunities in the Asia-Pacific ...
(Date:4/2/2015)... In the current eight ... the strongest competitors, showing outstanding performance in recognition ... is capable of a throughput of 5.000.000 fingerprints ... be ten times faster than most competitors and ...      (Photo: http://photos.prnewswire.com/prnh/20150402/738673-a ) , ...
Breaking Biology News(10 mins):HYPR Corp. Joins the FIDO Alliance 2HYPR Corp. Joins the FIDO Alliance 3NEC Security Competitive Profile 2015 2DERMALOG AFIS Emerges as Performance Winner in NIST Benchmark Test 2DERMALOG AFIS Emerges as Performance Winner in NIST Benchmark Test 3
... , LAS VEGAS , April 27 In its third ... open to a more inclusive pool of contestants and takes place three times ... Championship) invites any company with tea inventory in North America to ... harvests of hot tea, as well as iced tea. , ...
... April 27 From international jetsetter to model to entrepreneur, ... resume as she unveils her debut collection for QVC. The Slovenian beauty is scheduled ... PM (ET) to debut the Melania™ Timepieces & Fashion Jewelry line ... , , ...
... with a volume equivalent to 40 Amazon Rivers has been ... in the Indian Ocean sector of the Southern Ocean, 4200 ... Nature Geoscience , the researchers described the current more ... important pathway in a global network of ocean currents that ...
Cached Biology News:World Tea Expo Throws Open Door to Tea Championship 2Melania Trump Takes 'Time' To Shine With New QVC(R) Collection 2Melania Trump Takes 'Time' To Shine With New QVC(R) Collection 3Melania Trump Takes 'Time' To Shine With New QVC(R) Collection 4Massive Southern Ocean current discovered 2
... Rabbit polyclonal to BMP2 ... factor beta (TGFB) superfamily. Bone morphogenic ... a candidate gene for the autosomal ... progressiva. Immunogen: Synthetic ...
The Microseal 384 plate positioner ensures precise positioning of Microseal 384-well plates in the x-, y-, and z-dimensions, for accurate automated liquid handling....
...
... manual heat sealer ideal for low to medium ... seal a wide range of plates of differing ... element of the sealer is pre-set for optimal ... when used in conjunction with ABgenes sealing foil ...
Biology Products: