Navigation Links
High-Titer ,Retroviral Vectors for Gene Delivery


High transduction efficiency and predictable copy number

High-Titer Retroviral Vectors for Gene Delivery

Katherine Felts John C. Bauer Peter Vaillancourt
Stratagene

The high-titer retroviral vectors pFB and pFB-Neo can be used with any Moloney Murine Leukemia Virus (MMLV)-based packaging cell line. Virus produced with these vectors can be used to infect a wide range of cell types including human cells, and because MMLV-based virions are labile and readily inactivated by ethanol, ultraviolet light, and human complement, they are relatively safe and can be used in a P2 tissue culture facility. In transient virus production experiments, using a 293 cell-based producer line, we have achieved titers in excess of 10 8 colony-forming units per ml (cfu/ml) of supernatant for pFB, and greater than 107 cfu/ml for pFB-Neo. Protein expression from the viral promoter in infected cells was comparable to or higher than that for the vector pLXSN, another commercially available MMLV vector. The vector pFB has been deleted for all viral coding sequence and contains no exogenous sequence other than that for the multiple cloning site (MCS). As has been shown for other such minimal MMLV-based vectors, pFB is capable of insertions of up to 8 kb without appreciable loss of titer.1

The development of high-titer retroviral vectors that are capable of infecting a wide variety of cell types has had a tremendous impact on fields for which highly efficient gene delivery is essential.2 When tissue culture cells are infected with virus harboring a gene of interest, such experiments commonly result in transduction efficiencies of greater than 90%. An additional advantage of retroviral vectors is that the copy number of integrated provirus can be easily controlled
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8

Related biology technology :

1. Safe Production of High-Titer Retrovirus
2. Generate Adenovirus Vectors in E. coli by Homologous Recombination with the AdEasy Adenoviral Vector System
3. Versatile Vectors for Ponasterone A- Inducible Control of Gene Expression in Mammalian Cells
4. Epitope-Tagging Vectors for Functional Analysis in Yeast
5. New Mammalian Expression Vectors Employ Stable, High-Level Fluorescence Humanized Renilla GFP Reporter
6. Efficiently Insert Unique Restriction Sites into Plasmid Vectors
7. High-Efficiency Site-Directed Mutagenesis of Large Plasmid Vectors
8. Versatile Reporter Vectors for Monitoring Viral Transduction
9. Improved Vectors for PathDetect Trans-Reporting Systems
10. Mammalian Expression Vectors with Interchangeable Selectable Markers
11. Delivering siRNA Using Adenoviral Vectors
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: High Titer Retroviral Vectors for Gene Delivery

(Date:10/15/2008)... SUNNYVALE, Calif., Oct. 15 /PRNewswire-FirstCal....S. Food & Drug Administration (FDA) categorized,C...fection (SSTI) test as,"Moderate Complexity" under...LIA). The new test is designed for on-demand detec...s (MRSA) and Staphylococcus,aureus (SA), (typicall...
(Date:10/15/2008)... DUSSELDORF, Germany, October 15 /PRNewswire-Fir...2% to EUR268.0m, - Adjusted Group Results More Th...4m to EUR16.5m, - Sales Process Started for Techn...nfirms the Outlook for 2008, Gerresheimer AG agai...rating results in the third quarter of 2008. Overa...
(Date:10/14/2008)... Recently, zinc oxide (ZnO) nanowires have drawn m...re and unique optical and piezoelectric properties... conceived, including the next generation of field...s and resonators. ZnO nanowires are also envisione...semiconducting and piezoelectric properties. , R...
(Date:10/14/2008)... - Conference Call to Discuss Results to Be H...re-FirstCall/ -- ViroPharma Incorporated,s,(Nasdaq... are expected to be,released on Wednesday, October...rkets., The company will host a conference call a... the same day. During the conference call, ViroPha...
Breaking Biology Technology:Cepheid Molecular Diagnostic Test for MRSA and S. aureus Categorized 'Moderate Complexity' by FDA 2Cepheid Molecular Diagnostic Test for MRSA and S. aureus Categorized 'Moderate Complexity' by FDA 3Cepheid Molecular Diagnostic Test for MRSA and S. aureus Categorized 'Moderate Complexity' by FDA 4Gerresheimer Substantially Increases Quarterly Operating Profit 2Gerresheimer Substantially Increases Quarterly Operating Profit 3Gerresheimer Substantially Increases Quarterly Operating Profit 4Gerresheimer Substantially Increases Quarterly Operating Profit 5Strong elasticity size effects in ZnO nanowires 2Strong elasticity size effects in ZnO nanowires 3ViroPharma to Release 2008 Third Quarter Financial Results on October 29, 2008 2
...e" services can be profitable after all. Last week...that this month it would hit the elusive goal of e... a few years of hooking up business contacts for f...ces, ranging in cost from $60 to $2,000 per year. ... expectations of even the most optimistic at Linke...
... a biotechnology leader was greatly enhanced today...enza Viral Research will be established by UW-Madi...tor of University Research Park, where the researc...tute, which is designed to accelerate the pace of ... virus, and help prepare for a possible pandemic c...
...een battle now raging in the workplace. It pits in...ne side are managers who covet the traditional emp... - in return for a total commitment to playing the...ong hours in the office, grueling travel, and an o...the business requires. Only Type A,s need apply. ,...
... Inacom Information Systems was awarded Cisco Sys...ing company,s annual partner summit in San Diego, ...eless Makeover marketing campaign, which the compa...one, Dane Manufacturing, to be given an implementa... last November, and Inacom will hold a seminar in ...
Other Biology Technology:Free can be profitable 2Flu institute a booster shot for Wisconsin biotech stature 2Flu institute a booster shot for Wisconsin biotech stature 3Who will win the battle to redefine the workplace? 2Who will win the battle to redefine the workplace? 3Who will win the battle to redefine the workplace? 4Who will win the battle to redefine the workplace? 5
(Date:10/15/2008)...d and how species remain separate are crucial ques...osses between different animal species are often i...r example, cannot reproduce. A sheep-goat hybrid, ...is usually stillborn. Such hybrids can also be dys...ake are a mixture of sounds from both parent speci...
(Date:10/14/2008)... for finding life on other planets and in extreme ...ate,s new astrobiology initiative under a five-yea...gnatures of Life from Earth and Beyond." , Chris...s, will lead an interdisciplinary team to develop ...biological signatures in relevant ecosystems in an...
(Date:10/14/2008)...is available in Spanish . , An interdisciplina... the Institute for Research in Biomedicine (IRB Ba...tal d,Investigaci Qumica" (ICIQ, Tarragona), have ...in protein p53 stable even when it presents certai... Giralt, head of the Chemistry and Molecular Pharm...
(Date:10/14/2008)...4, 2008Three years after Hurricane Katrina devasta...s of the destruction of the city,s floodwall syste...tomologist (Vol. 54, No. 3) suggests that Formosa...uthor Gregg Henderson, a professor at the Louisian...ubterranean termites (Coptotermes formosanus Shira...
Breaking Biology News(10 mins):Turtle doves commit adultery 2Turtle doves commit adultery 3Penn State receives new NASA astrobiology grant 2Penn State receives new NASA astrobiology grant 3Design of a compound that stabilizes the main natural suppressor of tumors 2Did termites help Katrina destroy New Orleans floodwalls? 2Jefferson radiation oncologists use real time system to plant seeds against cancer 1792 1Jefferson radiation oncologists use real time system to plant seeds against cancer 1792 2Diet and medications may assist prevention of prostate cancer 1789 1Diet and medications may assist prevention of prostate cancer 1789 2Super Bowl Champion Pro Bowler and Pittsburgh Steeler Alan Faneca to Host Sixth Annual Bid for Hope Auction 1787 1Super Bowl Champion Pro Bowler and Pittsburgh Steeler Alan Faneca to Host Sixth Annual Bid for Hope Auction 1787 2NEWSWEEK 3A Cover 3A Giving Globally 3A How to Heal the World 1785 1NEWSWEEK 3A Cover 3A Giving Globally 3A How to Heal the World 1785 2NEWSWEEK 3A Cover 3A Giving Globally 3A How to Heal the World 1785 3
...ner knows--different plant species mature at diffe...in natural habitats call this phenomenon "compleme...se it reduces overlap in the time period when spec... posted online the week of Sept. 4 in the Proceedi...s working at Stanford,s Jasper Ridge Biological Pr...
...has a detrimental effect on a person,s ability to ...the September Journal of Nuclear Medicine. , "Usi...splanted heart as a very specific model to study t...c nervous system, we determined that reinnervation...replace damaged ones--is slower in diabetic patien...
...of events comprise many components--including sigh...eatures of an episodic memory are woven together i...tle understanding of how this binding takes place ...y center, the hippocampus. A central question has ... packet," or a collection of perceptual strands th...
...ng demand by consumers for healthier and more ecol... systems of packaging that prolong the useful life...ecyclable. Protection is currently carried out wit...re not completely biodegradable. One of the most p...dible coating ?a transparent film that covers the ...
Other Biology News:Global changes alter the timing of plant growth, scientists say 2Global changes alter the timing of plant growth, scientists say 3Diabetes slows nerve recovery after heart transplant 2How the brain weaves a memory 2Edible coatings will be the packaging of the future 2Edible coatings will be the packaging of the future 3Edible coatings will be the packaging of the future 4
Mimic Sf9 Insect Cells from Invitrogen
Propidium iodide; solution (Available only in the U.S.) from Roche Applied Science
...ighSens reagents and supplies, for 10 chips, provi...m high-sensitivity RNA analysis (picogram levels) ...resis system. Supplied are 1,250 microliters RNA g...ers RNA ladder, 900 microliters RNA HighSens loadi...
Anti-NCAPD3 Monoclonal AntibodyOther Sub-Family: not assigned-Other Applications: Immunoprecipitation, Western Blot
Biology Products: