High Throughput, Reproducible Results
A multiwell electroporator makes optimizing electroporation conditions for new cell types much easier than single well electroporators. In addition, high throughput electroporation of siRNA libraries into primary cells is an extremely effective research tool for investigating gene function across entire gene classes. The siPORTer-96 Electroporation Chamber allows screening of large numbers of siRNAs rapidly, reproducibly, and in parallel. Using the same pulse conditions in a single run, other parameters can be identical or varied across wells. The standard sample volume is just under 50 l, and as few as 2.5 x 104 cells can be used per reaction. This means that ~100 siRNAs could be screened using only 2.5 x 106 cells and 4.4 ml of siPORT siRNA Electroporation Buffer.
The siPORTer-96 Electroporation Chamber yields results
that are remarkably reproducible (Figure 2). 96 samples were electroporated
using identical conditions. Half of the samples received an siRNA targeting
GAPDH (Silencer GAPDH
siRNA, Cat# 4605), and half received a scrambled Negative Control
siRNA (Silencer Negative