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High Throughput siRNA Electroporation

NEW
siPORTer-96 Electroporation Chamber

Efficient siRNA delivery with minimal cell mortality
Electroporate up to 96 samples at the same time
Deliver siRNA into primary, hard-to-transfect, and suspension cells
Does not require expensive, consumable electroplates or cuvettes
Use with Bio-Rad Gene Pulser Xcell pulse generator


Electroporation: An Advantageous Alternative to Chemical Transfection

Human primary cells are often preferred for RNAi experiments because they are more similar to their in vivo counterparts than are immortalized cells. Although common chemical transfection methods are effective in many transformed cell lines, they are often not effective for primary cells [1]. For these cell types as well as suspension and other hard-to-transfect cells, electroporation is a valuable alternative.

Electroporation involves applying an electric field pulse to induce the formation of microscopic pores in the cell membrane. These pores allow nucleic acids such as siRNA to traverse the membrane. Under specific pulse conditions, the pores reseal, and the electroporated cells recover and resume growth.

The siPORTer-96 Electroporation Chamber (patent pending; Figure 1) works with the Bio-Rad Gene Pulser Xcell pulse generator to allow
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