Navigation Links
High Throughput siRNA Electroporation

NEW
siPORTer-96 Electroporation Chamber

Efficient siRNA delivery with minimal cell mortality
Electroporate up to 96 samples at the same time
Deliver siRNA into primary, hard-to-transfect, and suspension cells
Does not require expensive, consumable electroplates or cuvettes
Use with Bio-Rad Gene Pulser Xcell pulse generator


Electroporation: An Advantageous Alternative to Chemical Transfection

Human primary cells are often preferred for RNAi experiments because they are more similar to their in vivo counterparts than are immortalized cells. Although common chemical transfection methods are effective in many transformed cell lines, they are often not effective for primary cells [1]. For these cell types as well as suspension and other hard-to-transfect cells, electroporation is a valuable alternative.

Electroporation involves applying an electric field pulse to induce the formation of microscopic pores in the cell membrane. These pores allow nucleic acids such as siRNA to traverse the membrane. Under specific pulse conditions, the pores reseal, and the electroporated cells recover and resume growth.

The siPORTer-96 Electroporation Chamber (patent pending; Figure 1) works with the Bio-Rad Gene Pulser Xcell pulse generator to allow
'"/>

Source:


Page: All 1 2 3 4 5 6

Related biology technology :

1. Innovative Tissue Array Technology for High-Throughput Screening of Gene Expression
2. Highest Possible Transformation Efficiencies for High-Throughput Applications
3. High-Throughput Cultivation of Bacterial Clones
4. High-Throughput Isolation of Total RNA
5. High-Throughput System Generates Ultra-Pure PCR Products Suitable for All Applications
6. The DIG System Nonradioactive Automated High-Throughput In Situ Hybridization: a Powerful Tool for Functional Genomics Research
7. High Throughput aRNA Amplification
8. High Throughput siRNA Delivery In Vitro: From Cell Lines to Primary Cells
9. High Throughput RNA Isolation for High Performance Microarray Analysis
10. RNAi for High Throughput Analysis
11. Robust High Throughput RNA Isolation from Blood Samples
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: High Throughput siRNA Electroporation

(Date:11/24/2014)... Billerica, Massachusetts (PRWEB) November 24, 2014 ... Simplify Panels ” from METTLER TOLEDO demonstrates how ... panel. , Power analytics and sample panel design ... control cycle chemistry and protect against corrosion and ... take up a significant amount of panel space. ...
(Date:11/23/2014)... 23, 2014 Apiscent Labs, a ... fine chemical ingredients to the global pharmaceutical and ... its new website, Apiscent.com. , The Apiscent.com homepage ... clean design, easy navigation, rotating images and content ... stand out. , The company’s work ...
(Date:11/22/2014)... Boston, MA (PRWEB) November 21, 2014 ... and key investors will gather on December 3rd ... Boston Biotech Conferences series. GeneticRx will take place ... Harvard Medical School and will discuss the present ... gene therapy, exon skipping, and gene editing—as well ...
(Date:11/22/2014)... (PRWEB) November 21, 2014 On November ... at the 2014 Emerging Medical Technologies Summit in San ... Awards. Widely regarded among Silicon Valley investors and ... device innovation, the win also positions Briteseed to ... Innovator competition in 2015 and compete with other elite ...
Breaking Biology Technology:New Video Shows How to Simplify Cycle Chemistry Sample Panels to Save Time and Costs 2Pioneering Academics and Key Industry Leaders to Discuss Rare Diseases and the Emergence of New Genetic Medicines at Genetic Rx on December 3rd 2Insight Product Development Accelerator Member Wins MedTech Innovator Award Competition 2Insight Product Development Accelerator Member Wins MedTech Innovator Award Competition 3
... Nov. 6 Transgenomic (OTC,Bulletin Board: TBIO) today announced that ... Rodman & Renshaw 10th Annual,Healthcare Conference to be held November ... City. Mr. Tuttle and Debra Schneider, Chief,Financial Officer, will be ... Event: Rodman & Renshaw 10th Annual Healthcare ...
... Frost & Sullivan today,announced that the ... Award in,molecular/cellular imaging has been bestowed upon ... the targeting and imaging of apoptosis., ... is,an innovative set of rationally designed molecules ...
... http://www.amdl.com ),AMDL, Inc. (Amex: ADL ), a ... and the U.S., today announced its,newly appointed Chairman ... & Renshaw,s Annual Global Investment Conference taking place ... Palace Hotel in New York,City., Mr. MacLellan ...
Cached Biology Technology:Transgenomic to Present at Rodman & Renshaw 10th Annual Healthcare Conference 2Aposense Receives Frost & Sullivan Technology Innovation of the Year Award 2Aposense Receives Frost & Sullivan Technology Innovation of the Year Award 3Aposense Receives Frost & Sullivan Technology Innovation of the Year Award 4AMDL, Inc. New President & CEO Douglas MacLellan to Present at Rodman & Renshaw Annual Global Investment Conference November 12, 2008 2
(Date:11/10/2014)... U.S. store shelves in early 2010, and people have ... packets can be tossed into a washing machine without ... The convenience, though, has come with risks for young ... Children,s Hospital found that from 2012 through 2013, U.S. ... than 6 years of age swallowing, inhaling, or otherwise ...
(Date:11/7/2014)... 2014  In conjunction with the Glendale ... Community Foundation of the Verdugos has announced the " ... a $250,000 initiative to keep the Verdugo Regional Crime Lab ... six months of existence, the Lab has processed more than ... law enforcement. "Because our number one priority is ...
(Date:11/6/2014)... a team of Florida State University biologists could lead ... to and survive environmental swings such as droughts or ... of the journal The Plant Cell , sheds ... proteins) is organized in a cell and how plants ... on and others are turned off. , "If you ...
Breaking Biology News(10 mins):Study finds laundry detergent pods, serious poisoning risk for children 2Community Foundation Of The Verdugos Unveils Campaign To Fund Glendale-Burbank DNA Justice Project 2Maize analysis yields whole new world of genetic science 2
... According to a new market research ... / type (two-, three-, four- & five-factor), ... Travel & Immigration, Consumer Electronics and Commercial ... MarketsandMarkets ( http://www.marketsandmarkets.com ), the total market ...
... are named for their whirling movement on top of ... While many may have found the movements ... energy efficiencyuntil now, thanks to a study performed by ... mechanical, aerospace and biomedical engineering, at the University of ...
... issue of the journal Environmental Health Perspectives ... Marine Environment" a companion report by the Dartmouth-led Coastal ... that mercury released into the air and then deposited ... the U.S. and globally. Over the past ...
Cached Biology News:Multi-Factor Authentication Market Worth $5.45 Billion by 2017 2Multi-Factor Authentication Market Worth $5.45 Billion by 2017 3Multi-Factor Authentication Market Worth $5.45 Billion by 2017 4University of Tennessee engineering professor looks to whirligig beetle for bio-inspired robots 2Mercury releases contaminate ocean fish: Dartmouth-led effort publishes major findings 2Mercury releases contaminate ocean fish: Dartmouth-led effort publishes major findings 3Mercury releases contaminate ocean fish: Dartmouth-led effort publishes major findings 4
The new Silencer siRNA Starter Kit is perfect for researchers new to RNAi. It provides all of the reagents, protocols, and instructions necessary to get underway with siRNA transfection and ensure su...
... protein Vtg in plasma from juvenile or male ... endocrine disrupting chemicals (EDCs) with estrogenic effects in ... screening test for the estrogenic effects of EDCs ... is a double-antibody immunometric (sandwich) EIA for analyzing ...
... is a 17 amino acid synthetic peptide whose sequence is from ... to carboxy terminus): C - N(217) - Q - P ... A - T - P - N - Y - T ... be used for neutralization and control experiments with the polyclonal antibody ...
... for which no antibody exists? Having difficulty generating ... a variety of pharmaceutical, biotech and academic researchers ... technology and expertise in antibody generation to work ... RabMAb development technology , High affinity and high ...
Biology Products: