Navigation Links
High Throughput siRNA Delivery In Vitro: From Cell Lines to Primary Cells

addition the siPORTer-96 Electroporation Chamber facilitated high throughput delivery of siRNAs to primary and immortalized suspension cells. While transfection efficiency and cell viability are cell type specific for both high throughput delivery methods, both methods were highly reproducible. Coefficients of variance, measured from the percent of mRNA remaining after siRNA delivery, were similar between 96 well reverse transfection and 96 well electroporation.

The combination of high throughput chemical reverse transfection using high performance reagents such as siPORT NeoFX and electroporation using the siPORTer-96 Electroporation Chamber, siPORT siRNA Electroporation Buffer, and optimized electroporation parameters, enables researchers to screen siRNA libraries to identify genes in virtually any cellular process using virtually any cell type. The widespread application of this approach will make it possible to assign cellular functions to many or even most of the genes in the human, mouse, and rat genomes.

Figure 6. Schematic Diagrams of Standard and Reverse Transfection Procedures.


Scientific Contributors
Dmitriy Ovcharenko, Richard Jarvis, Scott Hunicke-Smith, Kevin Kelnar, and David Brown Ambion, Inc.


back to top


Ordering Information
Cat# Prod
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9 10

Related biology technology :

1. Innovative Tissue Array Technology for High-Throughput Screening of Gene Expression
2. Highest Possible Transformation Efficiencies for High-Throughput Applications
3. High-Throughput Cultivation of Bacterial Clones
4. High-Throughput Isolation of Total RNA
5. High-Throughput System Generates Ultra-Pure PCR Products Suitable for All Applications
6. The DIG System Nonradioactive Automated High-Throughput In Situ Hybridization: a Powerful Tool for Functional Genomics Research
7. High Throughput aRNA Amplification
8. High Throughput siRNA Electroporation
9. High Throughput RNA Isolation for High Performance Microarray Analysis
10. RNAi for High Throughput Analysis
11. Robust High Throughput RNA Isolation from Blood Samples
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: High Throughput siRNA Delivery Vitro From Cell Lines Primary Cells

(Date:5/21/2015)... 2015  CytRx Corporation (NASDAQ: CYTR ), ... oncology, today announced positive updated results from its ... the treatment of unresectable glioblastoma multiforme (GBM), a ... trial is designed to investigate the preliminary efficacy ... have progressed following prior treatment with surgery, radiation ...
(Date:5/21/2015)... May 21, 2015 Veolia’s environmental ... exclusive North American distribution agreement with VWR to ... , With more than 160 years of experience, ... services for laboratory and production facilities, has cultivated ... and differentiated services to enable science. Endetec’s ...
(Date:5/20/2015)... 2015 This year has been one ... US Patented Pearl’s Premium Ultra Low Maintenance Lawn ... country that are coming out of record bad weather. ... of major global concerns related to water, health, and ... the alternative to the standard, water-wasting, chemical-treated standard lawn. ...
(Date:5/20/2015)... May 20, 2015  Health eCareers, the healthcare ... organizations with job seekers looking for the flexibility ... new mobile and online tool dedicated exclusively to ... SHIFT enables employers to quickly find ... nursing, per diem and other temporary positions, eliminating ...
Breaking Biology Technology:CytRx Reports Positive Updated Phase 2 Aldoxorubicin Clinical Trial Results in Glioblastoma Multiforme (Brain Cancer) 2CytRx Reports Positive Updated Phase 2 Aldoxorubicin Clinical Trial Results in Glioblastoma Multiforme (Brain Cancer) 3CytRx Reports Positive Updated Phase 2 Aldoxorubicin Clinical Trial Results in Glioblastoma Multiforme (Brain Cancer) 4CytRx Reports Positive Updated Phase 2 Aldoxorubicin Clinical Trial Results in Glioblastoma Multiforme (Brain Cancer) 5CytRx Reports Positive Updated Phase 2 Aldoxorubicin Clinical Trial Results in Glioblastoma Multiforme (Brain Cancer) 6CytRx Reports Positive Updated Phase 2 Aldoxorubicin Clinical Trial Results in Glioblastoma Multiforme (Brain Cancer) 7Veolia’s TECTA™ B16 Instrument for E. Coli Detection is Offered Exclusively Through VWR 2Breakthrough 4th Generation Low Maintenance Lawn Seed Offers Solution to California Drought 2Health eCareers Launches SHIFT Mobile and Online Tool to Match Healthcare Employers with Temporary Job Seekers 2Health eCareers Launches SHIFT Mobile and Online Tool to Match Healthcare Employers with Temporary Job Seekers 3
... -- Wake Forest University has received the first patent for ... production of today,s flat cells at a fraction of the ... be green," said David Carroll, Ph.D., the director of Wake ... cell was developed. "This device can make a huge difference." ...
... ... industry and academia to the pioneering life sciences tool company. , ... San Diego, CA (PRWEB) April 7, ... world,s first 3D biosensor surface for label-free biomolecular interaction analysis, announced two additions to ...
... ... 1978 Murders , ... 6, 2010 -- In a stunning turn of events, the murder of five teens in 1978 ... Evans was the main suspect at the time of the initial investigation and took at least ...
Cached Biology Technology:Wake Forest earns patent for efficient, inexpensive fiber-based solar cells 2Silicon Kinetics Announces Addition of Dr. Rick Brown and Dr. Eric Topol to Its Board of Directors 2Silicon Kinetics Announces Addition of Dr. Rick Brown and Dr. Eric Topol to Its Board of Directors 3NACVSA: Killer of Five Teenagers Passes Polygraph 2NACVSA: Killer of Five Teenagers Passes Polygraph 3
(Date:5/8/2015)... SAN JOSE, Calif. , May 8, 2015 ... human interface solutions, today announced that members of the executive ... J.P. Morgan 43rd Annual Technology, Media and Telecom Conference ... Westin Waterfront Hotel in Boston, MA ... Date: May 27, 2015 Time: 2:45pm ET Location: The New ...
(Date:4/27/2015)... Apr. 27, 2015 NXT-ID, Inc. (NASDAQ: ... company focused on the growing mobile commerce market, announces ... pre-order customers the first week of May, 2015 and ... of May. Gino Pereira , ... for the company as Wocket® enters the consumer market. ...
(Date:4/17/2015)... , April 17, 2015 ... concerns, and technological advancement to drive biometric systems ... until 2020   According to a ... Biometric Systems Market Forecast &  Opportunities, 2020 ", biometric systems market in ... to register growth at CAGR of over 22% ...
Breaking Biology News(10 mins):Synaptics to Present at Upcoming Investor Conferences 2Wocket, the Smartest Wallet You Will Ever Own, Announces Shipment to Pre-order Customers 2Wocket, the Smartest Wallet You Will Ever Own, Announces Shipment to Pre-order Customers 3Saudi Arabia Biometric Systems Market to Grow at 22% Through 2020, Says TechSci Research 2Saudi Arabia Biometric Systems Market to Grow at 22% Through 2020, Says TechSci Research 3
... leisurely deep-sea diving trips and not from resurfacing too quickly, ... on the fossilized remains of Ichthyosarians , which were ... ago. The lesions were similar to those human ... and suggest the reptiles suffered from a version of ,the ...
... group of more than 30 researchers today gave a score ... of the world,s oceans, which showed the United States as ... recreation as leading concerns. The analysis, published in the ... scale in 10 separate categories such as clean water, biodiversity, ...
... fetal growth have consistently demonstrated deficits that persist through ... studies have been inconsistent. A new study of ... growth and body composition throughout childhood has found growth ... as well as a delay in weight gain during ...
Cached Biology News:'Dinosaur bends' caused by prolonged diving 2Rating of ocean health shows 'room for improvement' 2Tracking the effects of prenatal alcohol exposure through to 9 years of age 2Tracking the effects of prenatal alcohol exposure through to 9 years of age 3
Self Closing Forceps...
... steadylite plus assay system offers ... sensitivity, Designed for batch-processing systems, ... higher density microplates such as ... free, Convenient storage conditions ( ...
... most comprehensive, cost-effective solution to your ... unprecedented quality into both our products ... detection chemistries and antibodies that have ... It also includes our competitively priced ...
... Kit is uniquely formulated to give ... protein modification for optimal mass spectrometry ... with traditional silver stains are eliminated ... reagents used do not modify protein ...
Biology Products: