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High Throughput siRNA Delivery In Vitro: From Cell Lines to Primary Cells

addition the siPORTer-96 Electroporation Chamber facilitated high throughput delivery of siRNAs to primary and immortalized suspension cells. While transfection efficiency and cell viability are cell type specific for both high throughput delivery methods, both methods were highly reproducible. Coefficients of variance, measured from the percent of mRNA remaining after siRNA delivery, were similar between 96 well reverse transfection and 96 well electroporation.

The combination of high throughput chemical reverse transfection using high performance reagents such as siPORT NeoFX and electroporation using the siPORTer-96 Electroporation Chamber, siPORT siRNA Electroporation Buffer, and optimized electroporation parameters, enables researchers to screen siRNA libraries to identify genes in virtually any cellular process using virtually any cell type. The widespread application of this approach will make it possible to assign cellular functions to many or even most of the genes in the human, mouse, and rat genomes.

Figure 6. Schematic Diagrams of Standard and Reverse Transfection Procedures.


Scientific Contributors
Dmitriy Ovcharenko, Richard Jarvis, Scott Hunicke-Smith, Kevin Kelnar, and David Brown Ambion, Inc.


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