Navigation Links
High Throughput siRNA Delivery In Vitro: From Cell Lines to Primary Cells

ency maximizes reduction in target gene expression and improves the likelihood that a significant phenotype will be observed.

Reproducibility of siRNA delivery between wells and between plates reduces the variability of quantitative phenotypic results and improves the resolution of screening data.

Cell viability increases specificity of the siRNA effect, further improving the likelihood that significant phenotypic differences will be observed.


High Throughput Chemical Reverse Transfection--For Immortalized Cells

We adapted reverse transfection (a variation of standard transfection that involves transfection in suspension; see "Reverse Transfection" at right) to facilitate high throughput siRNA delivery. Because the procedure features minimal pipetting steps and can be performed using multi-channel pipettors, reverse transfection can be used to deliver siRNAs to approximately 1000 cell samples per hour without robotics. It is also amenable to automation

Efficiency of High Throughput Reverse Transfection. To assess transfection efficiency, we mixed different concentrations of a well-characterized GAPDH siRNA with the versatile siPORT NeoFX Transfection Agent in wells of a 96 well plate. Five different cell types were added to the siRNA/lipid complexes to effect reverse transfection.
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9 10

Related biology technology :

1. Innovative Tissue Array Technology for High-Throughput Screening of Gene Expression
2. Highest Possible Transformation Efficiencies for High-Throughput Applications
3. High-Throughput Cultivation of Bacterial Clones
4. High-Throughput Isolation of Total RNA
5. High-Throughput System Generates Ultra-Pure PCR Products Suitable for All Applications
6. The DIG System Nonradioactive Automated High-Throughput In Situ Hybridization: a Powerful Tool for Functional Genomics Research
7. High Throughput aRNA Amplification
8. High Throughput siRNA Electroporation
9. High Throughput RNA Isolation for High Performance Microarray Analysis
10. RNAi for High Throughput Analysis
11. Robust High Throughput RNA Isolation from Blood Samples
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: High Throughput siRNA Delivery Vitro From Cell Lines Primary Cells

(Date:9/30/2014)... Using a bio-mimicking analog of one of ... an international research team led by Alejandro Briseno ... a major step in developing long-sought polymer architecture ... for use in electronic devices. , Briseno, ... and others at Stanford University and Dresden University ...
(Date:9/30/2014)... 30, 2014 Nematicide is a type ... are microscopic parasitic roundworms, found in massive quantities ... plants and animals. Nematicides have tended to be ... promoting migration through the soil. A single gram ... nematodes. With more than 10,000 species classified, they ...
(Date:9/30/2014)... Sept. 30, 2014  RegeneRx Biopharmaceuticals, Inc. (OTC: ... it has received a Canadian Patent for Inhibition ... This patent includes claims using thymosin beta 4, ... peptides for these purposes.  The patent will expire ... Inc. ( www.regenerx.com ) RegeneRx ...
(Date:9/30/2014)... Angeles, CA (PRWEB) September 30, 2014 ... (EWS), the high-speed, chemical-free process to clean up large ... to shareholders, CEO Riggs Eckelberry reported on the launch ... Texas, at a site provided by partner STW ... up to 1000 barrels per day (bpd) and achieved ...
Breaking Biology Technology:Blades of grass inspire advance in organic solar cells 2Blades of grass inspire advance in organic solar cells 3Nematicides Market by Types (Fumigants, Organophosphates, Carbamate and Bio-Nematicides), Applications (Agrochemicals, Industrial, and Others), and Geography (North America, Europe, Asia-Pacific & ROW) - Global Trends & Forecasts to 2019 2RegeneRx Receives Canadian Patent for Inhibition or Reversal of Skin Aging 2OriginOil, Inc. Report to Shareholders: Blue Gold in West Texas 2
... Biotechnologies AB, an,atherosclerosis R&D company developing novel products ... today that it has,signed two distributor agreements - ... Ltd. , Carina Schmidt, ... about the,opportunities these agreements present to Athera. They ...
... that Understanding the Fundamental Mechanism of Angiogenesis is ... - AAmgen Launches Angiogenesis Website THOUSAND OAKS, Calif., ... ) today announced that results from several preclinical ... presented at the 2009 American Association for Cancer ...
... SAN FRANCISCO, Calif., April 16 Proteolix, Inc. today ... post of Senior Vice President, Finance and Corporate Development ... the company,s clinical development and regulatory leadership team with ... the post of Chief Medical Officer (CMO) and Albert ...
Cached Biology Technology:Athera Signs Agreements With German and UK Distributors for New Cardiovascular Biomarker 2Athera Signs Agreements With German and UK Distributors for New Cardiovascular Biomarker 3Preclinical Data From Amgen's Oncology Pipeline to be Presented at AACR 2Preclinical Data From Amgen's Oncology Pipeline to be Presented at AACR 3Preclinical Data From Amgen's Oncology Pipeline to be Presented at AACR 4Preclinical Data From Amgen's Oncology Pipeline to be Presented at AACR 5Preclinical Data From Amgen's Oncology Pipeline to be Presented at AACR 6Preclinical Data From Amgen's Oncology Pipeline to be Presented at AACR 7Proteolix Announces New Appointment in Finance and Expands Clinical and Regulatory Leadership Team 2Proteolix Announces New Appointment in Finance and Expands Clinical and Regulatory Leadership Team 3Proteolix Announces New Appointment in Finance and Expands Clinical and Regulatory Leadership Team 4
(Date:9/30/2014)... Calif. The National Institutes of Health (NIH) ... today to develop an electrode array system that ... brain works through unprecedented resolution and scale. , ... to support President Obama,s BRAIN (Brain Research ... research effort to revolutionize our understanding of the ...
(Date:9/30/2014)... live in almost any aquatic environment on Earth, but ... species are pushed to the limit. The amount of ... critical for how different species cope in the future, ... of Gothenburg, published in the scientific journal Proceedings ... continues apace thanks to increasing levels of greenhouse gases ...
(Date:9/30/2014)... want your doctor to know what goes wrong with your ... idea to know what "normal" actually is. That,s where a ... the FASEB Journal comes in. In ... key set of molecules that can help scientists "see" which ... What,s more, they found never-before-detected gene activity and that men ...
Breaking Biology News(10 mins):NIH taps lab to develop sophisticated electrode array system to monitor brain act 2NIH taps lab to develop sophisticated electrode array system to monitor brain act 3Shape up quickly -- applies to fish, too! 2Scientists identify which genes are active in muscles of men and women 2
... paleobiologist J. William Schopf and colleagues have produced 3-D images ... old -- preserved in rocks, an achievement that has never ... Mars brings rocks back to Earth, Schopf said the techniques ... spectroscopy, could enable scientists to look at microscopic fossils inside ...
... National Jewish Medical and Research Center report in the ... promising proteins, known as fibulins 3 and 5, slow ... blood vessels from sprouting. The proteins are promising candidates ... produce fibulin proteins, which regulate cell proliferation, migration and ...
... be potentially life threatening in the home, but the presence ... Mike Steven and colleagues from the University of Nottingham have ... act as an early warning of leaks in natural gas ... satellite remote systems to monitor gas leaks via the spectrum ...
Cached Biology News:Inside rocks, implications for finding life on Mars 2Proteins stop blood-vessel and tumor growth in mice 2Plants used to detect gas leaks, from outer space! 2
... This CLS number ... number, created to easily ... If showing no availability ... the old Sigma-Aldrich number ...
This CLS number is a new product number, created to easily match Cornings product number. If showing no availability yet, please order under the old Sigma-Aldrich number (E6027) or contact customer s...
... pressure gradient system uses three Adept CE ... a flow rate in one microlitre increaments. ... added flexibility. System control and data processing ... interface unit, CE 4900., Column heater/chillers can be ...
... of large numbers of samples may be carried ... minimum operator attention. System control and data ... CE 4900. The elegant, powerful and very ... and Windows 98 and 2000., The supplied detector ...
Biology Products: