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High Throughput aRNA Amplification

MessageAmp II-96 aRNA Amplification Kit


Amplify as little as 100 ng of input RNA in a single round for GeneChip analysis

Includes ArrayScript, an engineered M-MLV RT, to maximize yields of full-length cDNA

aRNA synthesis powered by MEGAscript High Yield Transcription Kit, the best technology for in vitro transcription

High labeling efficiency yields consistently high percent present calls and low 3'/5' ratios in GeneChip analysis


MessageAmp II-96 aRNA Amplification Kit is the first kit designed for high throughput amplification of mRNA and is based on the patented Van Gelder-Eberwine linear RNA amplification method. This kit takes advantage of extensive improvements incorporated into the MessageAmp II aRNA Kit combined with a new magnetic bead-based purification of both the cDNA and the aRNA.

The new microplate format available with MessageAmp II-96 provides a simple, streamlined procedure for the entire aRNA amplification process, including first and second strand cDNA synthesis, in vitro transcription, and aRNA labeling. Figure 1 illustrates aRNA yields obtained with either the manual or the fully-automated MessageAmp II-96 protocol at various HeLa total RNA input levels for both 4 and 14 hour IVT reactions. Figure 2 compares the correlation coefficients (% present calls on Affymetrix Human Genome Focus Arrays) obtained using MessageAmp II-96 (Plate 1 and Plate 2) versus Ambion's st
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