Navigation Links
High Throughput aRNA Amplification

MessageAmp II-96 aRNA Amplification Kit


Amplify as little as 100 ng of input RNA in a single round for GeneChip analysis

Includes ArrayScript, an engineered M-MLV RT, to maximize yields of full-length cDNA

aRNA synthesis powered by MEGAscript High Yield Transcription Kit, the best technology for in vitro transcription

High labeling efficiency yields consistently high percent present calls and low 3'/5' ratios in GeneChip analysis


MessageAmp II-96 aRNA Amplification Kit is the first kit designed for high throughput amplification of mRNA and is based on the patented Van Gelder-Eberwine linear RNA amplification method. This kit takes advantage of extensive improvements incorporated into the MessageAmp II aRNA Kit combined with a new magnetic bead-based purification of both the cDNA and the aRNA.

The new microplate format available with MessageAmp II-96 provides a simple, streamlined procedure for the entire aRNA amplification process, including first and second strand cDNA synthesis, in vitro transcription, and aRNA labeling. Figure 1 illustrates aRNA yields obtained with either the manual or the fully-automated MessageAmp II-96 protocol at various HeLa total RNA input levels for both 4 and 14 hour IVT reactions. Figure 2 compares the correlation coefficients (% present calls on Affymetrix Human Genome Focus Arrays) obtained using MessageAmp II-96 (Plate 1 and Plate 2) versus Ambion's st andard single-tube MessageAmp II reaction.


[click here to enlarge]

Figure 1. Robotic or Manual RNA Amplification using MessageAmpII-96 aRNA Amplification Kit. Twelve replicates of HeLa cell total RNA inputs (5, 1, 0.3, and 0.1 g) were processed according to the MessageAmp II-96 protocol either on the Biomek 2000 (Robot, Beckman-Coulter) or manually (human). In vitro transcription (IVT) times of (A) 14 hours and (B) 4 hours were used. Following aRNA clean-up, yield was assessed by measuring UV absorbance at 260 nm.


[click here to enlarge]

Figure 2. Correlation Coefficients of aRNA Data Obtained with MessageAmp II-96 Kit. HeLa cell total RNA (Control RNA included in MessageAmp II-96 aRNA Amplification Kit) was used to prepare biotin-labeled aRNA following the standard MessageAmp II-96 protocol (Plate-1 and Plate-2) and MessageAmp II protocol (tube). aRNA (10 g) was analyzed on an Affymetrix Human Genome Focus Array, and the correlation coefficients were calculated for the percent present calls.


Magnetic beads are used for both the cDNA and aRNA purification steps, eliminating the need for vacuum filtration or centrifugation. The use of beads also enables elution into smaller volumes, thus providing a higher concentration of aRNA. The entire process can be performed either manually or fully automated using a robot integrated with a thermal cycler and orbital shaker. Each kit contains sufficient reagents to amplify 100 samples, thus providing four samples for pre-testing prior to using a 96 well format.


Scientific Contributors

Chris Willis, Sharmili Moturi, Xingwang Fang Ambion, Inc.


back to top


Ordering Information
Cat# Product Name Size 1819 MessageAmp II-96 aRNA Amplification Kit 100 rxns 10027 Magnetic Stand-96 1 each 10050 96 well Magnetic-Ring Stand 1 each
'"/>

Source:


Page: All 1 2 3

Related biology technology :

1. Innovative Tissue Array Technology for High-Throughput Screening of Gene Expression
2. Highest Possible Transformation Efficiencies for High-Throughput Applications
3. High-Throughput Cultivation of Bacterial Clones
4. High-Throughput Isolation of Total RNA
5. High-Throughput System Generates Ultra-Pure PCR Products Suitable for All Applications
6. The DIG System Nonradioactive Automated High-Throughput In Situ Hybridization: a Powerful Tool for Functional Genomics Research
7. High Throughput siRNA Electroporation
8. High Throughput siRNA Delivery In Vitro: From Cell Lines to Primary Cells
9. High Throughput RNA Isolation for High Performance Microarray Analysis
10. RNAi for High Throughput Analysis
11. Robust High Throughput RNA Isolation from Blood Samples
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:3/24/2017)... , March 24, 2017 On ... trading session at 5,817.69, down 0.07%; the Dow Jones ... and the S&P 500 closed at 2,345.96, marginally dropping ... sectors closed in green, 4 sectors finished in red, ... Friday, Stock-Callers.com has initiated reports coverage on the following ...
(Date:3/23/2017)... , ... March 23, 2017 , ... ... material that exhibits both viscous and elastic characteristics when deformed, which is identical ... exhibits properties to gently absorb compressive forces and return to its natural state ...
(Date:3/23/2017)... 23, 2017  Northwest Biotherapeutics (OTCQB: NWBO) (NW ... therapies for solid tumor cancers, today announced that ... it announced last Friday, March 17, 2017. ... investors securities totaling 28,843,692 shares, comprised of 18,843,692 ... shares of Class C Warrants pre-funded at the ...
(Date:3/23/2017)...  BioPharmX Corporation (NYSE MKT: BPMX), a specialty ... today reported financial results for the quarter and ... an update on the company,s clinical development efforts ... are pleased to report that last year was ... Anja Krammer. "We achieved key clinical milestones and ...
Breaking Biology Technology:
(Date:3/7/2017)... England , March 7, 2017 Brandwatch , ... chosen by The Prince,s Trust to uncover insights to ... across The Trust. The UK,s leading youth charity will ... social campaign results and get a better understanding of the topics ... ...
(Date:3/2/2017)... 2017 Summary This report provides ... KGaA and its partnering interests and activities since 2010. ... Description The Partnering Deals and Alliance since 2010 report ... one of the world,s leading life sciences companies. ... to ensure inclusion of the most up to date ...
(Date:2/28/2017)... --  Acuant , a leading provider of data capture ... new and core technologies building upon the acquisition of ... desktop Acuant FRM TM facial recognition and match ... manual review of identity documents by accredited professionals. ... most accurate capture software to streamline workflows by securely ...
Breaking Biology News(10 mins):