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High-Throughput System Generates Ultra-Pure PCR Products Suitable for All,,,Applications

n was added directly to both PCR mixtures, and were loaded into adjacent wells of a StrataPrep 96 PCR binding plate. The wells were washed, and the PCR products were eluted. Figure 4 shows quantitative and equivalent recovery of a 229-bp PCR product. The unpurified PCR product is shown in Lane 2 to compare recovery of the purified samples. The purified products in Lanes 3 and 4 were from identical amplifications with and without an oil overlay, respectively. This data demonstrates that the samples of varying condition in Lanes 2, 3, and 4 are equally well purified. The resulting purified DNA was successfully digested to completion with a variety of restriction enzymes (Figure 4, Lanes 5 through 14).

Fig.4

Highly Pure DNA Ideal for Automated Sequencing

PCR products were amplified from a plasmid DNA template and purified with either the StrataPrep 96 PCR purification kit or Qiagens QIAquick 96 PCR purification kit. The purified samples were sequenced with the automated fluorescent dideoxynucleotide method, and the data were compared. Figure 5 shows that the StrataPrep 96 PCR kit resulted in highly purified DNA with sequence data showing low-background, high-signal intensity data with no ambiguities in over 500 bases. In contrast, the QIAquick 96 PCR kit demonstrated sequence data that had high background, variable signal intensity, and numerous ambiguities.

Fig.5

Conclusions

The StrataPrep 96 PCR purification kit is a fast and efficient system for generating ultrapure DNA in a high-throughput format, processing of as many as 96 samples. This kit uses the same successful principles of a silica fiber-matrix binding membrane as
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