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High-Throughput System Generates Ultra-Pure PCR Products Suitable for All,,,Applications

l. As seen in Figure 2, the wells that were processed without samples show no contamination products from adjacent wells. Furthermore, the ethidium bromide staining contaminant present in the unpurified sample, migrating in advance of the 250-bp marker (Lane 2), is eliminated from wells of the StrataPrep 96 plate (Lanes 3, 5, 7, 9, 11, and 13).

Fig.2

Consistent Results in Variable Conditions

The performance of the StrataPrep 96 PCR kit was evaluated by first determining the DNA binding capacity of the kits binding plate. Increasing quantities of linear double-stranded plasmid DNA were loaded into plate wells. The wells were washed, DNA was eluted, and the recovered DNA was quantitated by UV spectrophotometry (data not shown). The maximum amount of DNA recovered was 5 g per well. The size range of DNA recovered was also determined by loading a mixture of linear double-stranded DNA ranging in size from 115 bp to 23,000 bp into each well of the binding plate. The plate was washed, DNA was eluted, and DNA recovery from 15 randomly chosen wells was assessed by gel electrophoresis. Figure 3 shows uniform recovery of all DNA sizes from the wells of the plate.

Fig.3

One time-consuming step in most purification schemes is removing the oil overlay from amplification reactions. Although this step is a nuisance, it is often essential for recovering PCR products of sufficient purity for subsequent applications. Without modifying the standard purification procedure, this variable was examined by purifying two 50-l amplification reactions, one with and one without the oil overlay. An equal volume of DNA binding solutio
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