For many disciplines, including molecular biology, biochemistry, neuroscience, and clinical diagnostics, the need to screen and process many PCR products simultaneously has significantly increased. Stratagene has responded to this need by transforming the StrataPrep Kit technology into a high-throughput format, making it possible to process as many as 96 samples simultaneously. In the StrataPrep 96 PCR purification procedure (Figure 1), an equal volume of DNA binding solution is added directly to PCR reactions (with or without an oil overlay). The entire mixture is then transferred to a StrataPrep 96 Kit binding plate. After washing to remove contaminants, PCR products are eluted in a small volume of low-ionic-strength buffer and are ready for many applications such as restriction digestion and automated fluorescent dideoxynucleotide sequencing.
The StrataPrep 96 PCR purification kit includes 96-well binding plates, 96-well collection and waste plates, plate sealers, storage mats, and all necessary buffers. As an added convenience, the kits components are compatible with most 96-well plate vacuum manifolds and 96-well-adapted centrifuges and can be adapted to a robotic system, such as the Biomek 2000 workstation (Beckman Coulter)
The 96-well plates have innovative drip directors that channel
liquid flow out of the wells into corresponding wells of collection plates.
To demonstrate that these drip directors eliminate well-to-well contamination
between samples, 2.3-kb PCR products were purified in alternate wells
of a StrataPrep 96 PCR purification plate. The plate was processed as
if all wells contained PCR products, and samples from each well were visualized
on an agarose ge