Navigation Links
High-Throughput System Generates Ultra-Pure PCR Products Suitable for All,,,Applications

For many disciplines, including molecular biology, biochemistry, neuroscience, and clinical diagnostics, the need to screen and process many PCR products simultaneously has significantly increased. Stratagene has responded to this need by transforming the StrataPrep Kit technology into a high-throughput format, making it possible to process as many as 96 samples simultaneously. In the StrataPrep 96 PCR purification procedure (Figure 1), an equal volume of DNA binding solution is added directly to PCR reactions (with or without an oil overlay). The entire mixture is then transferred to a StrataPrep 96 Kit binding plate. After washing to remove contaminants, PCR products are eluted in a small volume of low-ionic-strength buffer and are ready for many applications such as restriction digestion and automated fluorescent dideoxynucleotide sequencing.

The StrataPrep 96 PCR purification kit includes 96-well binding plates, 96-well collection and waste plates, plate sealers, storage mats, and all necessary buffers. As an added convenience, the kits components are compatible with most 96-well plate vacuum manifolds and 96-well-adapted centrifuges and can be adapted to a robotic system, such as the Biomek 2000 workstation (Beckman Coulter)

Ninety-Six Independent Samples

The 96-well plates have innovative drip directors that channel liquid flow out of the wells into corresponding wells of collection plates. To demonstrate that these drip directors eliminate well-to-well contamination between samples, 2.3-kb PCR products were purified in alternate wells of a StrataPrep 96 PCR purification plate. The plate was processed as if all wells contained PCR products, and samples from each well were visualized on an agarose ge
'"/>

Source:


Page: All 1 2 3 4 5

Related biology technology :

1. Innovative Tissue Array Technology for High-Throughput Screening of Gene Expression
2. Highest Possible Transformation Efficiencies for High-Throughput Applications
3. High-Throughput Cultivation of Bacterial Clones
4. High-Throughput Isolation of Total RNA
5. The DIG System Nonradioactive Automated High-Throughput In Situ Hybridization: a Powerful Tool for Functional Genomics Research
6. Advantages of ReadyAgarose Precast Gels Over 96-Well Handcast Gels for High-Throughput Analysis, Rev A
7. New Mammalian Two-Hybrid System Detects Protein-Protein Interactions
8. Assess the In Vivo Activation of Signal Transduction Pathways with PathDetect Reporting Systems
9. Generate Adenovirus Vectors in E. coli by Homologous Recombination with the AdEasy Adenoviral Vector System
10. New Yeast Cloning System for Producing Proteins with Native Amino Acid Sequences
11. Enhanced PCR Cloning System
Post Your Comments:
(Date:1/22/2015)... 21, 2015 Cambridge Semantics, the leading provider ... announced that 2014 was a record-breaking year across the board ... the Anzo Smart Data Platform and our Smart Data solutions, ... from diverse data which led to record growth for the ...
(Date:1/22/2015)... California (PRWEB) January 22, 2015 Pipette.com ... Offer to their comprehensive portfolio of Eppendorf products. ... customers to purchase a Centrifuge 5424/5424 R and receive ... of either Eppendorf Research plus or Eppendorf Reference 2 ...
(Date:1/22/2015)... Geneva, Switzerland (PRWEB) January 22, 2015 ... for characterized mammalian Research Cell Banks (RCBs) used for ... generated Research Cell Banks will include Next-Generation Sequencing ... cell’s genetic architecture de-risks biologic manufacturing by ensuring the ...
(Date:1/22/2015)... Jan. 22. 2015  Varian Medical Systems (NYSE: VAR ... honored for its commitment to sustainability with inclusion on a ... the highest ranked healthcare equipment company among the Corporate Knights ... World Economic Forum at Davos, Switzerland . ...
Breaking Biology Technology:Cambridge Semantics Announces Record Results for 2014 2Eppendorf Announces Promotional Bundle Which Includes: Eppendorf Centrifuge 5424, 3-Pack of Pipettes, and Tips - Available Now at Pipette.com 2Selexis Generated Research Cell Banks Now Fully Sequenced Using Next-Generation Sequencing 2Varian Honored Among World's 100 Most Sustainable Corporations 2
... one of the largest provider of integrated and customized ... and investors that, on April 10, 2008, the National ... 264,000 m3 of,biodiesel, for delivery between July 1 and ... ensure supply of the necessary volumes for the,mandatory blend ...
... LAUSANNE, Switzerland, April 11 Galderma Pharma ... dermatology, today,announced that its U.S. holding company, ... CollaGenex Pharmaceuticals, Inc. (Nasdaq:,CGPI). As a result ... wholly owned,subsidiary of Galderma Laboratories., "We ...
... international start-up, CEOs, business development executives, and service providers ... ... the globe, MASSAPEQUA, N.Y. and CLEVELAND, April ... BioEnterprise Corporation, the Cleveland-based bioscience business,formation, recruitment, and acceleration initiative, ...
Cached Biology Technology:Galderma Completes Acquisition of CollaGenex 2Galderma Completes Acquisition of CollaGenex 3Galderma Completes Acquisition of CollaGenex 4IBF Conferences Inc*International Business Forum and BioEnterprise Corporation present the 2nd Annual Global Healthcare Investing Conference 2IBF Conferences Inc*International Business Forum and BioEnterprise Corporation present the 2nd Annual Global Healthcare Investing Conference 3
(Date:1/22/2015)...   EyeLock, Inc. , a market leader of iris-based identity ... to the new role of Senior Vice President of ... of mobile platforms and wearable solutions for EyeLock,s technologies. Gerber ... the semiconductor industry to his role at EyeLock, with expertise ...
(Date:12/24/2014)... , Dec. 23, 2014  Since its launch in December 2014, ... to eliminate the pain of trying to remember their usernames ... biometrics fused to their smartphones. To assist people who have ... the company that created 1U and focuses on redefining identity, ...
(Date:12/22/2014)... Research and Markets ( http://www.researchandmarkets.com/research/fx4bg3/the_global ) ... Global Watermarking and Fingerprinting Markets" report to ... This insight provides an overview ... markets. Watermarking aims to control and monitor piracy ...
Breaking Biology News(10 mins):EyeLock Names Steve Gerber as Senior Vice President of Mobile and Wearables 21U Offers Best Solution to the Username / Password Dilemma: For FREE! 21U Offers Best Solution to the Username / Password Dilemma: For FREE! 3The Global Watermarking and Fingerprinting Markets 2
... HONOLULU April 25, 2012 -- Many shark populations have ... excessive harvesting for their fins, as an incidental catch ... is particularly true for oceanic species. However, until now, a ... of Pacific reef sharks at a large geographic scale. ...
... disease normally found in subarctic climates has been identified for ... south as Herefordshire., Sclerotinia subarctica , a fungus known ... found in carrots and meadow buttercups in Perthshire by plant ... Life Sciences at the University of Warwick. , It is ...
... research sailboat gliding over glassy seas in the Pacific ... was littered with confetti-size pieces of plastic debris, until ... the particles disappeared. After taking samples of ... Proskurowski, a researcher at the University of Washington, discovered ...
Cached Biology News:Scientists provide first large-scale estimate of reef shark losses in the Pacific Ocean 2Wind pushes plastics deeper into oceans, driving trash estimates up 2Wind pushes plastics deeper into oceans, driving trash estimates up 3
... Reagent Grade C 19 H 9 Br ... PRODUCT SPECIFICATIONS Form: Powder Clarity of Solution: ... 3.0 - Yellow pH 4.6 - ... in agarose and acrylamide gel electrophoresis. Used ...
tracking dye in electrophoresis; free acid, C 19 H 10 BrO 5 S; MW: 669.96...
Denhardt's Solution is a mixture of blocking agents used in membrane-based hybridization protocols. The solution contains 1% Ficoll (type 400) 1% polyvinylpyrrolidone and 1% bovine serum albumin. Ste...
... ELF 97 Cytological Labeling Kit provides a ... detection of cellular targets, including cell-surface receptors ... Labeling Kit contains the patented ELF 97 ... yellow-green precipitate at the site of enzymatic ...
Biology Products: