Navigation Links
High-Throughput System Generates Ultra-Pure PCR Products Suitable for All,,,Applications


Simultaneous purification of 96 PCR products

Scott Basehore Jeff Braman
Stratagene

Andrew Firmin
Phenogenex

The StrataPrep 96 PCR purification kit is a new member of the StrataPrep product line. This kit offers a high-throughput format for purifying ultra-pure PCR product DNA from large numbers of samples. The StrataPrep 96 PCR purification kit uses the same silica matrix-binding technology as the other StrataPrep Kit products, but in a 96-well plate format. A vacuum manifold and centrifuge are used to recover 5 g per well of DNA ranging in size from approximately 100 bp to 23,000 bp. Purified PCR products are free of PCR artifacts as well as contaminants from adjacent wells and oil used in some PCR protocols. The purified PCR products are at an ideal concentration for automated fluorescent dideoxynucleotide sequencing. Better sequence data was obtained with a template purified with the StrataPrep 96 PCR kit, compared to the same PCR template with another commercially available kit.

In 1997, Stratagene introduced the StrataPrep PCR purification kit1, an effective miniprep-scale tool for purifying PCR products in applications such as cloning and automated fluorescent dideoxynucleotide sequencing2. The basis of this successful product is its unique glass fiber matrix, which captures DNA in the presence of a chaotropic salt. With the kits wash solution, unincorporated amplification primers and nucleotides, buffer components, enzymes, and nonspecific amplification products smaller than 100 bp can be removed from the matrix-bound PCR products. To recover the desired PCR products, low-ionic-strength buffer is added to the fiber matrix. This step is followed by centrifugation to collect the DNA at a concentration that is suitable for many applications.

Fig.1
'"/>

Source:


Page: All 1 2 3 4 5

Related biology technology :

1. Innovative Tissue Array Technology for High-Throughput Screening of Gene Expression
2. Highest Possible Transformation Efficiencies for High-Throughput Applications
3. High-Throughput Cultivation of Bacterial Clones
4. High-Throughput Isolation of Total RNA
5. The DIG System Nonradioactive Automated High-Throughput In Situ Hybridization: a Powerful Tool for Functional Genomics Research
6. Advantages of ReadyAgarose Precast Gels Over 96-Well Handcast Gels for High-Throughput Analysis, Rev A
7. New Mammalian Two-Hybrid System Detects Protein-Protein Interactions
8. Assess the In Vivo Activation of Signal Transduction Pathways with PathDetect Reporting Systems
9. Generate Adenovirus Vectors in E. coli by Homologous Recombination with the AdEasy Adenoviral Vector System
10. New Yeast Cloning System for Producing Proteins with Native Amino Acid Sequences
11. Enhanced PCR Cloning System
Post Your Comments:
(Date:10/25/2014)... 24, 2014 Research and Markets ... by Product , Research Area & by End User ... offering. In this report, the global ... research areas, and end users. The product segments of ... instruments & consumables, kits, and reagents. The subsegments of ...
(Date:10/22/2014)... 2014 New test innovations will be the ... (IVD) test industry, especially as companies struggle with reimbursement ... more than 25 testing innovations in important disease areas ... IVD products. Kalorama details the new approaches its biennial survey ... In Vitro Diagnostic Tests , 9th Edition . ...
(Date:10/22/2014)... DUBLIN and BOSTON , ... ACT ), a leading global specialty pharmaceutical company, and ... been granted an exclusive option to acquire Rhythm,s wholly ... (RM-131), a peptide ghrelin agonist, for the treatment of ... Rhythm has completed a successful Phase 2 trial ...
(Date:10/22/2014)... (PRWEB) October 21, 2014 The North ... concerned market North America with analysis and forecast of ... 2013, and is expected to reach $623.6 million by ... 2018. , Browse through the TOC of the North ... of the in-depth analysis provided. This also provides a ...
Breaking Biology Technology:Global Epigenetics (VLA, WBA, NBA, RTA, Rotary-Wing Aircraft) Market - Forecast to 2019 2Global Epigenetics (VLA, WBA, NBA, RTA, Rotary-Wing Aircraft) Market - Forecast to 2019 3Report: Radical New IVD Test Approaches Key To Growth 2Report: Radical New IVD Test Approaches Key To Growth 3Actavis Signs Option to Acquire Rhythm Subsidiary and its GI Development Product, Relamorelin 2Actavis Signs Option to Acquire Rhythm Subsidiary and its GI Development Product, Relamorelin 3Actavis Signs Option to Acquire Rhythm Subsidiary and its GI Development Product, Relamorelin 4Actavis Signs Option to Acquire Rhythm Subsidiary and its GI Development Product, Relamorelin 5The North American crystal oscillator market is expected to reach $623.6 million by 2018 - New Report by MicroMarket Monitor 2The North American crystal oscillator market is expected to reach $623.6 million by 2018 - New Report by MicroMarket Monitor 3The North American crystal oscillator market is expected to reach $623.6 million by 2018 - New Report by MicroMarket Monitor 4
... ), a biopharmaceutical company focused on the treatment of obesity, today announced that the Company will be speaking ... , , Date:  May ... Time:  8:30am Pacific Time , , , ... , , ...
... HONG KONG , May 10 /PRNewswire-FirstCall/ ... Cypress Biosciences ("Cypress"), its development partner for Indaflex, that ... the termination of all Indaflex activity in the ... Chinese market. AlphaRx is working diligently with its regulatory ...
... NEW YORK , May 10 /PRNewswire-Asia-FirstCall/ -- ... pharmaceutical,company dedicated to improving health through the development, manufacture and,commercialization of a broad ... the first quarter ended March,31, 2010 . , ... First Quarter 2010 Financial Performance , ...
Cached Biology Technology:Orexigen(R) Therapeutics to Speak at Ninth Annual JMP Securities Research Conference 2American Oriental Bioengineering Reports First Quarter 2010 Financial Results 2American Oriental Bioengineering Reports First Quarter 2010 Financial Results 3American Oriental Bioengineering Reports First Quarter 2010 Financial Results 4American Oriental Bioengineering Reports First Quarter 2010 Financial Results 5American Oriental Bioengineering Reports First Quarter 2010 Financial Results 6American Oriental Bioengineering Reports First Quarter 2010 Financial Results 7American Oriental Bioengineering Reports First Quarter 2010 Financial Results 8
(Date:10/17/2014)... release is available in German . ... a very few drugs. When treating overdoses, doctors are often ... especially difficult if there is a combination of drugs involved. ... and accidentally swallows his grandmother,s pills? ETH professor Jean-Christophe Leroux ... to find an answer to this question. "The task was ...
(Date:10/16/2014)... thinking on how the bowel lining develops and, in ... cancer starts. , The researchers produced evidence that stem ... that are a feature of the bowel lining, and ... development, a controversial finding as scientists are still divided ... technologies, Dr Chin Wee Tan and Professor Tony Burgess ...
(Date:10/16/2014)... SHELTON, Conn. , October 16, 2014 ... ("NXT-ID" or the "Company"), a biometric authentication company focused ... present its leading edge technologies including Wocket™, the Smart ... Review® (FiRe) Conferenece. FiRe is a leading global conference ... Hosted by Mark Anderson , founder ...
Breaking Biology News(10 mins):Emergency aid for overdoses 2Emergency aid for overdoses 3Cryptic clues drive new theory of bowel cancer development 2NXT-ID Inc. to Present at 13th Annual FiRe Conference 2NXT-ID Inc. to Present at 13th Annual FiRe Conference 3
... next five years for a pilot project to assess ... conditions can and should become a routine part of ... launched today by the National Institutes of Health to ... as part of, or instead of, the current newborn screening ...
... September 5, 2013, Shenzhen, China BGI in collaboration ... other Chinese institutes, have completed the genome sequencing of ... infectious disease tuberculosis (TB). The study published online in ... researchers to better understand the genetic basis underlying drug ...
... known for some time that the blood vessels that transport ... are more than just bodily pipelines. Arterioles and capillaries, the ... flow of the blood they,re carrying. Biomedical engineers at Drexel ... that explains just how they do it. The team, ...
Cached Biology News:UCSF receives $4.5M to study value of gene sequencing in newborns 2UCSF receives $4.5M to study value of gene sequencing in newborns 3UCSF receives $4.5M to study value of gene sequencing in newborns 4Drug resistance-associated genes: A cornerstone for the control and protection against tuberculosis 2Drexel researchers looking inside vessels to understand blood's ebb and flow 2
Secure-Seal™ hybridization chamber gasket, eight chambers, 9 mm diameter, 0.8 mm deep *set of 20*...
RAT ANTI MOUSE F4/80 ANTIGEN:BIOTIN Immunogen: Thioglycollate stimulated peritoneal macrophages from C57/BL mice...
ANTI PIG LDH H4...
... ideal for use in refrigerators, freezers, or liquid nitrogen. ... colors allow for color coding to project, user, lab, ... or promote cool air circulation. Supplied with 10 x ... Box dimensions: 13 x 13 x 5.1 ...
Biology Products: