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High Throughput RNA Isolation for High Performance Microarray Analysis

e 2B) showed excellent correlation. High Percent Present calls, >60%, were also obtained for total RNA purified using the MagMAX procedures and Protocol A (Figure 2C).

Figure 2. Generate High Quality Microarray Data Using Purified Total RNA from MagMAX-96 for Microarrays Kits. RNA, isolated from frozen mouse kidney (1 g, each sample), was amplified using MessageAmp II-96 aRNA Amplification Kit. The biotin labeled amplified RNA was fragmented and hybridized to an Affymetrix GeneChip Mouse Genome 430A 2.0 Array (total probe set=22,000). (A) Scatter plot of normalized signal intensities of two mouse kidney total RNA samples purified using MagMAX-96 for Microarrays; correlation between samples is 0.999. (B) Scatter plot of normalized signal intensities of two mouse kidney total RNA samples purified using MagMAX-96 for Microarrays and the Affymetrix recommended protocol; correlation between methods is 0.998. (C) Percent Present calls for each sample purified using all three methods; >60% Present calls were obtained for all methods.


MagMAX-96 for Microarrays

The MagMAX-96 for Microarray Kit comes with sufficient reagents for 96 purifications from fresh, flash-frozen, or RNAlater-treated tissue samples in a high throughput format. RNAlater-treated samples must be processed with the Spin protocol. The purified total RNA is suitable for microarray analysis, quantitative RT-PCR, and other downstream RNA applications.


Scientific Contributors

Quoc Hoang, WeiWei Xu, Roy Chris Willis,
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