Navigation Links
High Throughput RNA Isolation for High Performance Microarray Analysis

MagMAX-96 for Microarrays

Protocol combines benefits of TRI Reagent sample lysis with MagMAX magnetic bead-based RNA isolation technology

Procedure is compatible with fresh, flash-frozen, or RNAlater-treated tissues

Streamlined procedure allows high throughput processing using multichannel pipettes or automated platforms

Simple, Fast, and Efficient Protocol

The new MagMAX-96 for Microarrays Kit streamlines RNA isolation without sacrificing RNA quality or quantity. The Spin protocol uses TRI Reagent (see RNA, DNA, and Protein from a Single Sample) for sample lysis (<20 mg tissue or 1 x 106 cells) and MagMAX magnetic beads for RNA purification. Magnetic beads can be placed directly into TRI Reagent lysates for RNA purification, eliminating organic extractions, which enables high throughput processing of lysates by manual, multichannel pipetting or automated liquid handling systems.

The alternative No-Spin protocol for smaller samples (<5 mg tissue or 5 x 105 cells) eliminates a 10 minute centrifugation step, uses a DNase treatment, and permits fully automated sample processing.

Excellent RNA Yield and Integrity

Hi gh quality and yield of total RNA is critical for successful gene expression profiling experiments. RNA quality is assessed by the A260/A280 ratio, the ratio of 28S:18S rRNA peaks, and RNA Integrity Number (RIN). As shown in Figure 1, the MagMAX-96 for Microarrays Spin and No-Spin Procedures both purify RNA with good yield and quality. RIN is based on the entire electrophoretic trace of the RNA sample (1=highly degraded and 10=highly intact). Total RNA purified using MagMAX-96 for Microarrays has high integrity (RIN typically >7.8).

Figure 1. Obtain RNA of High Quality and Yield with MagMAX-96 for Microarrays Total RNA Isolation Kit. RNA was isolated from frozen mouse liver with the MagMAX-96 for Microarrays Kit. Tissue homogenates were processed in quadruplets or octuplets using the Spin procedure (homogenate derived from 10 mg tissue per sample) or the No-Spin procedure (homogenate derived from 5 mg tissue per sample), respectively. Purified RNA (2 l) was quantified using a NanoDrop Spectrophotometer (A, B, and C). The ratio of 28S to 18S rRNA was determined with an RNA LabChip Kit and the Agilent 2100 bioanalyzer.

Successful Microarray Experiments

Total RNA isolated from mouse kidney using MagMAX-96 for Microarrays or the Affymetrix recommended protocol (Protocol A: phenol-based sample lysis followed by glass fiber filter RNA purification) were compared by microarray analysis. Normalized microarray signal intensities obtained from two total RNA samples isolated using MagMAX-96 for Microarrays (Figure 2A) and from total RNA samples purified using MagMAX-96 for Microarrays and Protocol A (Figur e 2B) showed excellent correlation. High Percent Present calls, >60%, were also obtained for total RNA purified using the MagMAX procedures and Protocol A (Figure 2C).

Figure 2. Generate High Quality Microarray Data Using Purified Total RNA from MagMAX-96 for Microarrays Kits. RNA, isolated from frozen mouse kidney (1 g, each sample), was amplified using MessageAmp II-96 aRNA Amplification Kit. The biotin labeled amplified RNA was fragmented and hybridized to an Affymetrix GeneChip Mouse Genome 430A 2.0 Array (total probe set=22,000). (A) Scatter plot of normalized signal intensities of two mouse kidney total RNA samples purified using MagMAX-96 for Microarrays; correlation between samples is 0.999. (B) Scatter plot of normalized signal intensities of two mouse kidney total RNA samples purified using MagMAX-96 for Microarrays and the Affymetrix recommended protocol; correlation between methods is 0.998. (C) Percent Present calls for each sample purified using all three methods; >60% Present calls were obtained for all methods.

MagMAX-96 for Microarrays

The MagMAX-96 for Microarray Kit comes with sufficient reagents for 96 purifications from fresh, flash-frozen, or RNAlater-treated tissue samples in a high throughput format. RNAlater-treated samples must be processed with the Spin protocol. The purified total RNA is suitable for microarray analysis, quantitative RT-PCR, and other downstream RNA applications.

Scientific Contributors

Quoc Hoang, WeiWei Xu, Roy Chris Willis, Angela Burrell, Mangkey Bounpheng, Xiaowei Wang, Xingwang Fang Ambion, Inc.

back to top

Ordering Information
Cat# Product Name Size 1819 MessageAmp II-96 aRNA Amplification Kit 100 rxns 1839 MagMAX-96 for Microarrays Total RNA Isolation Kit 96 purifications 10050 96 well Magnetic-Ring Stand 1 each


Page: All 1 2 3 4

Related biology technology :

1. Innovative Tissue Array Technology for High-Throughput Screening of Gene Expression
2. Highest Possible Transformation Efficiencies for High-Throughput Applications
3. High-Throughput Cultivation of Bacterial Clones
4. High-Throughput Isolation of Total RNA
5. High-Throughput System Generates Ultra-Pure PCR Products Suitable for All Applications
6. The DIG System Nonradioactive Automated High-Throughput In Situ Hybridization: a Powerful Tool for Functional Genomics Research
7. High Throughput aRNA Amplification
8. High Throughput siRNA Electroporation
9. High Throughput siRNA Delivery In Vitro: From Cell Lines to Primary Cells
10. RNAi for High Throughput Analysis
11. Robust High Throughput RNA Isolation from Blood Samples
Post Your Comments:

(Date:11/25/2015)... DIEGO , Nov. 25, 2015 Orexigen® ... management will participate in a fireside chat discussion at ... New York . The discussion is scheduled ... .  A replay will be ... Media Contact:McDavid Stilwell  , Julie NormartVP, Corporate Communications ...
(Date:11/24/2015)... SUNNYVALE, Calif. , Nov. 24, 2015 ... executives will be speaking at the following conference, and ... New York, NY      Tuesday, December 1, ... New York, NY      Tuesday, December 1, ...      Piper Jaffray Healthcare Conference, New York, NY ...
(Date:11/24/2015)... 2015 /PRNewswire/ - Aeterna Zentaris Inc. (NASDAQ:  AEZS) (TSX: AEZ) ... of the Toronto Stock Exchange, confirms that as of ... corporate developments that would cause the recent movements in ... --> About Aeterna Zentaris Inc. ... Aeterna Zentaris is a specialty biopharmaceutical company engaged ...
(Date:11/24/2015)... Massachusetts , November 24, 2015 SHPG ... will participate in the Piper Jaffray 27 th Annual Healthcare ... Tuesday, December 1, 2015, at 8:30 a.m. EST (1:30 p.m. GMT). ... , Chief Financial Officer, will participate in the Piper Jaffray 27 ... , NY on Tuesday, December 1, 2015, at 8:30 a.m. ...
Breaking Biology Technology:
(Date:11/16/2015)... 16, 2015  Synaptics Inc. (NASDAQ: SYNA ... today announced expansion of its TDDI product portfolio ... controller and display driver integration (TDDI) solutions designed ... new TDDI products add to the previously-announced ... (WQHD resolution), and TD4322 (FHD resolution) solutions. All ...
(Date:11/10/2015)... LONDON , Nov. 10, 2015 /PRNewswire/ ... segmented on the basis of product, type, ... segments included in this report are consumables, ... this report are safety biomarkers, efficacy biomarkers, ... in this report are diagnostics development, drug ...
(Date:11/2/2015)... Nov. 2, 2015  SRI International has been awarded ... preclinical development services to the National Cancer Institute (NCI) ... provide scientific expertise, modern testing and support facilities, and ... pharmacology and toxicology studies to evaluate potential cancer prevention ... The PREVENT Cancer Drug Development Program is an NCI-supported ...
Breaking Biology News(10 mins):