Navigation Links
High Throughput Plasmid DNA Preparation Using A Magnetic Bead Kit On An Automated Workstation

erforming closely to Agencourt's internal procedures. Figure 1 is a sequence trace from a sample purified on the Sciclone. Read lengths are comparable at 789 versus 779 bp for the DNATrack and the Sciclone, respectively. Each system gave a 94% pass rate out of 384 samplings (4 x 96 well plates). Nucleic acid yield for the two systems are roughly 16 ng/μL by PicoGreen binding analysis. Results from electrophoretic separation of the purified nucleic acid are presented in Figure 2.

Discussion

The Agencourt CosMCPrep Kit, Agencourt ring magnet plate, and Sciclone ALH 3000 can easily purify plasmids. The system does not require external hardware such as a centrifuge or vacuum pump/filter. The ring magnet locator easily allows complete removal of supernatant. Selective removal of lysate was accomplished by blowing air into the wells of the culture plate at the 12 o' clock position. Bubbles push residual cell material to the top of the well while leaving clean lysate at the bottom for easy transfer.

PicoGreen and sequencing data demonstrates that the CaliperLS Sciclone ALH performs comparably to Agencourt's procedures and hardware. Sequencing lengths and total yield were comparable on the two systems.


'"/>

Source:


Page: All 1 2 3

Related biology technology :

1. Innovative Tissue Array Technology for High-Throughput Screening of Gene Expression
2. Highest Possible Transformation Efficiencies for High-Throughput Applications
3. High-Throughput Cultivation of Bacterial Clones
4. High-Throughput Isolation of Total RNA
5. High-Throughput System Generates Ultra-Pure PCR Products Suitable for All Applications
6. The DIG System Nonradioactive Automated High-Throughput In Situ Hybridization: a Powerful Tool for Functional Genomics Research
7. High Throughput aRNA Amplification
8. High Throughput siRNA Electroporation
9. High Throughput siRNA Delivery In Vitro: From Cell Lines to Primary Cells
10. High Throughput RNA Isolation for High Performance Microarray Analysis
11. RNAi for High Throughput Analysis
Post Your Comments:
(Date:12/22/2014)... Dec. 22, 2014  ( www.competitivehealth.com ) — Competitive ... medical bill review and advocacy service, has signed an ... WellCard Savings discount health services marketplace. 63% ... more than they expected to pay. As part of ... costs, WellCard Savings is pleased to offer medical bill ...
(Date:12/22/2014)... 22, 2014 Fertility Associates of Memphis ... Bank USA , a network of more than 20 ... partnership, patients have fast access to a national pool ... IVF . Fertility Associates of Memphis is the only ... treatment option. , Dr. William Kutteh , director ...
(Date:12/19/2014)... BioPlus Specialty Pharmacy (BioPlus), one ... of Nick Maroulis, Pharm.D. to the newly created position ... In this position, Dr. Maroulis will continue to manage ... of our multi-site pharmacies as the company expands. Dr. ... that time he has served in many different capacities ...
(Date:12/19/2014)... Dec. 19, 2014 Bina Technologies, Inc. (Bina, ... that they have been acquired by Roche (SIX: RO, ... privately held company that provides a big data platform ... (NGS) data for the academic and translational research markets. ... and will continue to focus on development of their ...
Breaking Biology Technology:WellCard Savings Announces CoPatient Medical Bill Review Service for Members 2Fertility Associates of Memphis Announces Partnership with Donor Egg Bank USA 2Fertility Associates of Memphis Announces Partnership with Donor Egg Bank USA 3BioPlus Specialty Pharmacy Promotes Dr. Nick Maroulis to Vice President of Specialty Pharmacy Services 2Bina Technologies is acquired by Roche. 2Bina Technologies is acquired by Roche. 3
... Meditor Pharmaceuticals Ltd.,announced today the appointment of Prof. Arie ... company., Dr. Schachner has been a member of ... a more active role in the Clinical Development,program., ... Cardiac Surgery,Department at Wolfson Medical Center and Tel Aviv ...
... Round of Financing for $3 Million, PITTSBURGH, ... Pathology, Inc.,s PathFinderTG(R) have fueled the,company,s expansion and ... growth of the company,s work force which now ... this backdrop, the company announced,today that it has ...
... N.Y., May 12 "What,s the difference between ... my DNA?" Hundreds of,women are trying to crack ... give me beautiful, healthy skin?, Bio-scientist turned ... expertise -- more than 20 years of researching, ...
Cached Biology Technology:Two New Appointments at Meditor Pharmaceuticals Ltd. 2Expansion and Relocation to New, State-Of-The-Art Facilities Marks Milestone in Effort to Roll Out New Tool for Cancer Diagnosis 2Expansion and Relocation to New, State-Of-The-Art Facilities Marks Milestone in Effort to Roll Out New Tool for Cancer Diagnosis 3A Scientist Dechipers the Code to Beautiful, Healthy Skin in 'The New Science of Perfect Skin' 2
(Date:12/11/2014)... ST. PAUL, Minn. , Dec. 10, 2014 ... in wireless physiologic monitoring, has released a new ... changing needs of preclinical toxicology researchers. M series, ... to help toxicologists collect the best possible physiologic ... studies. Adding functional endpoints to toxicology ...
(Date:12/10/2014)... Dec. 08, 2014 Research and Markets ... of the "Biometrics Market in Japan 2014-2018" ... http://photos.prnewswire.com/prnh/20130307/600769 The integration ... as rural banking and upgradation of the driver,s ... in the market. Besides the aforementioned projects, biometrics ...
(Date:12/3/2014)... IRVINE, Calif. , Dec. 2, 2014 /PRNewswire/ ... of globally deployed, innovative test solutions for military, ... the newest version of its successful TS-900 ... the performance and features of high-end systems to ... with outstanding value compared to traditional ATE. ...
Breaking Biology News(10 mins):New telemetry implants expected to change how large animal toxicology studies are conducted 2Biometrics Market in Japan 2014-2018: Key Vendors are DDS, Fujitsu, Hitachi and NEC 2Marvin Test Solutions Brings New Capabilities to PXI-Based Semiconductor Test with TS-960 2Marvin Test Solutions Brings New Capabilities to PXI-Based Semiconductor Test with TS-960 3
... of your time among stores, restaurants and skyscrapers, it may ... boots. A new study in Psychological Science , a ... time in nature may be more beneficial for mental processes ... John Jonides, and Stephen Kaplan from the University of Michigan ...
... HARBOR, N.Y. (Dec. 18, 2008) "Life happens ... build, process, activate, and inactivate; they polymerize, repair, support, ... cleave, inhibit, digest, fluoresce, induce, excise, carry, and repress; ... countless more activities." This excerpt is from the ...
... can we maximise the value and use of ... is the theme of a conference being held ... Co-organised by PHOEBE (Promoting Harmonisation of Epidemiological ... BBMRI (Biobanking and Biomolecular Resources Research Infrastructure), the ...
Cached Biology News:New manual presents robust, state-of-the-art proteomics methods for teaching and research 2Harmonizing Biobank Research Conference in 2009 2Harmonizing Biobank Research Conference in 2009 3
... enzyme that catalyzes the hydrolysis of,starch ... and dextrins. The,level of a-amylase in ... is of,clinical significance in the diagnosis ... plant and microbial a-amylases are,important enzymes ...
Rat monoclonal [20d5] to NKG2A + C+ E ( Abpromise for all tested applications). Antigen: Tissue / cell preparation (Mouse): CHO transfected cells expressing the B6 allele of NKG2A. Entrez Gen...
... The Thermo Labsystems Wellmix is a ... and time settings. The shaking speed is ... shaking frequency, high speed and a small ... assays. The shaking time is quick and ...
... Fluoroskan Ascent FL ... fluorometric and luminometric measurement ... long list of advanced ... and Luminoskan Ascent. Fluoroskan ...
Biology Products: