( isolation kit from Qiagen. RNA isolatio...)High-Throughput Isolation of Total RNA

isolation kit from Qiagen. RNA isolations were performed according to each kits instructions. The RNeasy 96 kit does not include DNase but does make available a supplemental protocol for DNase treatment; hence, we were able to carry out an RNA isolation with as well as without DNase treatment.

The StrataPrep 96 kit, with DNase treatment, generated RNA yields similar to the RNeasy 96 kit without DNase treatment (Table 3). When DNase treatment was included with the RNeasy 96 protocol, RNA yields decreased. The StrataPrep 96 kit samples have the highest RNA concentrations, which is especially important for analyzing small samples.

Table 3: Average Yields from Total RNA Purification Kits*

* RNA was isolated from NIH/3T3 cells grown in 96-well plates (approximately 3.8 x 10⁴ cells/well). The numbers are averages of three samples containing four pooled samples each. Yields were determined by spectrophotometric analysis.

The alternate protocol for the StrataPrep 96 kit was also tested (additional materials required).

Yield (g/well)

Concentration (g/ml)

A₂₆₀/A₂₈₀

StrataPrep kit

1.0 0.02

40 1.0

2.0 0.02

StrataPrep, spin only

0.9 0.07

48 2.0

2.1
'"/>

Source:

Page: All 1 2 3 4 5 6 7
Related biology technology :

1. Innovative Tissue Array Technology for High-Throughput Screening of Gene Expression
2. Highest Possible Transformation Efficiencies for High-Throughput Applications
3. High-Throughput Cultivation of Bacterial Clones
4. High-Throughput System Generates Ultra-Pure PCR Products Suitable for All Applications
5. The DIG System Nonradioactive Automated High-Throughput In Situ Hybridization: a Powerful Tool for Functional Genomics Research
6. Advantages of ReadyAgarose Precast Gels Over 96-Well Handcast Gels for High-Throughput Analysis, Rev A
7. Fast and Easy Isolation of PCR-Ready Genomic DNA from Whole Blood
8. Simple Isolation of RNA from Tissue and Cultured Cells
9. Fast Isolation of Total RNA from Small Numbers of Cells
10. Comparison of Growth Techniques and Media for the Purpose of Plasmid Isolation from E. coli Using the Eppendorf Perfectprep Plasmid Mini Kit
11. Isolation of Microorganisms