( le 2 ) were quantitated in f...)High-Throughput Isolation of Total RNA

le 2) were quantitated in fluorescent assays using the RiboGreen RNA quantitation kit (Molecular Probes) since absorbance measurements cannot be taken for such small samples. These samples also reflect yields of approximately 10^-5 g of total RNA per cell.

Table 2: Average Yields of RNA from Small Numbers of Cells (ng)*

* RNA was quantitated by the RiboGreen assay using dilutions of an rRNA standard.

Numbers in parentheses indicate the number of isolations performed.

Cell line

10⁴ cells

10³ cells

10² cells

10¹ cells

HeLa

119 (6)

9.6 (6)

1.0 (6)

0.211 (2)

HL-60

53 (1)

4.8 (1)

0.53 (1)

THP-1

84 (1)

6.9 (1)

0.97 (1)

0.110 (1)

NIH/3T3

325 (4)

23.3 (4)

2.9 (4)

Representative RNAs (Table 1) were electrophoresed
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