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High-Throughput Isolation of Total RNA

s

Cell line

5 x 105 cells

2 x 105 cells

1 x 105 cells

Mouse

NIH/3T3

11.0 (4)*

4.1 (4)

2.9 (4)

Hamster

CHO

4.7 (60)

Human

HeLa

9.2 (3)

5.5 (1)

2.1 (3)

Human

HL-60

4.0 (3)

1.9 (3)

1.1 (3)

Human

THP-1

6.4 (1)

1.6 (1)

0.8 (1)

Table 1 and Table 2 show total RNA yields from different cell numbers of a variety of cell lines. A typical mammalian cell contains approximately 10-5 g of total RNA.1 The yields for all cell lines are close to or higher than this number, indicating high yields. The A260/A280 ratios measured for samples isolated from 1 x 105 to 5 x 105 cells is greater than 1.9 for all samples, indicating high-quality RNA. Samples isolated from 1 x 101 to 1 x 104 cells (Tab
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