Navigation Links
High-Throughput Cultivation of Bacterial Clones

XL1-Blue bacteria containing the pBluescript plasmid. One ml of LB medium, containing 100 mg/ml ampicillin, was added to each 2-ml well of the 96-well plate. Bacteria were inoculated into alternate wells of the plate at a density of 2 105 cells/ml. The plate was sealed with its flexible plastic lid, which is designed to seal each well to prevent cross contamination. Perforations were made in the lid over the first 48 wells to allow an exchange of gases between the air space above the culture medium and the atmosphere. The 96-deep-well plate was then incubated at 37C in the thermoshaker (600 rpm).

Bacteria at the same cell density were inoculated into a 2-liter flask, which held 1 liter of LB medium containing 100 g/ml ampicillin. The flask was covered with a piece of sterile aluminum foil and incubated in a New Brunswick Scientific Model G25 Series floor incubator-shaker at 300 rpm.

Bacterial growth was monitored at various time points by removing samples from the aerated and nonaerated wells of both the plate and flask, then measuring absorption at 550 nm.1 Bacteria cultured in nonaerated wells of the plate were removed by perforating the plate lid with a hot needle just prior to sampling. This method prevented the need to remove the lid from the plate and introduce air into the nonaerated wells of the plate.

Bacterial Growth Rates

Figure 2 demonstrates equivalent growth rates of bacteria in early- and mid-log phases in aerated and nonaerated wells of the plate. Late-log phase growth rates of bacteria cultured in nonaerated wells were only slightly slower than in aerated wells. Cell densities at the end of the incubation period in aerated and nonaerated wells were identical. Flask-cultured bacteria demonstrated a slower initial rate of growth compared to plate-cultu
'"/>

Source:


Page: All 1 2 3

Related biology technology :

1. Innovative Tissue Array Technology for High-Throughput Screening of Gene Expression
2. Highest Possible Transformation Efficiencies for High-Throughput Applications
3. High-Throughput Isolation of Total RNA
4. High-Throughput System Generates Ultra-Pure PCR Products Suitable for All Applications
5. The DIG System Nonradioactive Automated High-Throughput In Situ Hybridization: a Powerful Tool for Functional Genomics Research
6. Advantages of ReadyAgarose Precast Gels Over 96-Well Handcast Gels for High-Throughput Analysis, Rev A
7. Mammalian and Bacterial Expression in One Vector
8. Bacterial mRNA Isolation, Fast and Easy
9. Bacterial RNA Isolation From Infected Eukaryotic Hosts
10. Bacterial RNA Isolation From Infected Eukaryotic Hosts
11. Bacterial Whole Genome Array Analysis
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: High Throughput Cultivation Bacterial Clones

(Date:8/26/2014)... -- An analysis by Replikins, Ltd. of Pubmed data ... virus strains has revealed extensive conservation and sharing ... and sharing of specific Replikin gene structures has permitted ... it did for the H5N1 influenza vaccine and the ... found to be effective (1,2). This ...
(Date:8/26/2014)... of engineers from Singapore has successfully piloted the ... satellite communications. , With the weight of payloads ... a lightweight, low power-consuming, wireless communication system to ... always been a challenge for system designers. , ... of Nanyang Technological University in Singapore launched VELOX-I, ...
(Date:8/26/2014)... , August 26, 2014 , ... NHS hospitals with surgery validation using its Stratasys Objet24 and ... Performing surgery on 3D printed models prior to operations reduces ... an effective aid for both patient and surgeon   ... ), a global leader of 3D printing and additive manufacturing ...
(Date:8/26/2014)... , Aug. 26, 2014  ViveBio, LLC ... sample collection, stabilization and transportation, announced today that ... Procurement Services, LLC (LPS) of Knoxville, ... products in the United States ... class of ambient temperature dried biological specimen transport ...
Breaking Biology Technology:ZigBee in the Sky 2Twelve UK Hospitals Enjoy Reduced Surgical Procedure Times and Decline in Costs by Implementing Stratasys 3D Printing Solutions 2Twelve UK Hospitals Enjoy Reduced Surgical Procedure Times and Decline in Costs by Implementing Stratasys 3D Printing Solutions 3Twelve UK Hospitals Enjoy Reduced Surgical Procedure Times and Decline in Costs by Implementing Stratasys 3D Printing Solutions 4Twelve UK Hospitals Enjoy Reduced Surgical Procedure Times and Decline in Costs by Implementing Stratasys 3D Printing Solutions 5ViveBio and Laboratory Procurement Services Sign Agreement for Distribution of the ViveST Line of Products 2
... , NEW YORK , July 27 ... announced the completion of a $5 million round ... Alleghany Corporation (N Y SE: Y), and other investors. ... leading position in the global online patent validation sector by ...
... /PRNewswire-FirstCall/ - Oncothyreon Inc. (Nasdaq: ONTY ) will conduct a conference call ... 2010 at 4:30 p.m. Eastern time ( 1:30 p.m. Pacific ... To participate in the ... 287-9361 (International). In addition, the call will be webcast live and can be ...
... physicists and materials scientists at the University of ... to rapidly grow centimeter-scale membranes of binary nanocrystal ... nanocrystals on a liquid surface. The study ... long-range-ordered BNSL membranes with rigorous control of nanocrystal ...
Cached Biology Technology:Article One Announces Completion of Funding from Alleghany Corporation 2Article One Announces Completion of Funding from Alleghany Corporation 3Penn collaboration leads to simpler method for building varieties of nanocrystal superlattices 2Penn collaboration leads to simpler method for building varieties of nanocrystal superlattices 3
(Date:8/25/2014)... PULLMAN, Wash.Five years ago this month, one of the ... the Washington State University campus, striking some 2,000 people. ... trove of data gathered at the time to gain ... launch the virus,s rapid spread across the university community., ... first cases came in to the university,s Health and ...
(Date:8/25/2014)... Researchers at the University of California, San Diego School of ... certain glioblastomas the primary form of a deadly brain ... not in the DNA sequence of the tumor, but in ... a priority report in the journal Oncotarget . ... by sequencing the DNA of the cancer cell," said Clark ...
(Date:8/25/2014)... family of proteins that promotes virus entry into cells ... HIV and other viruses, University of Missouri researchers have ... new insights into our understanding of not only HIV ... said Shan-Lu Liu, M.D., Ph.D., associate professor in the ... Immunology. , The study was recently published in the ...
Breaking Biology News(10 mins):WSU flu outbreak provides rare study material 2WSU flu outbreak provides rare study material 3Finding keys to glioblastoma therapeutic resistance 2MU researchers discover protein's ability to inhibit HIV release 2MU researchers discover protein's ability to inhibit HIV release 3
... shines a blue light on yeast and mammalian cells in her ... The effect is the result of a light-activated switch from a ... use this novel "on-off switch" to control cell growth or death, ... cells, said Tucker, an assistant research professor in the biology department ...
... The ocean surface is 30 percent more acidic today than it ... 50 years - a rising trend that could both harm coral ... the ocean food web, scientists warn. Despite the seriousness of ... deploy a complete suite of available tools to monitor rising acidification ...
... have found new evidence linking "Snowball Earth" glacial events to ... the National Science Foundation (NSF). Study results appear in ... The controversial Snowball Earth hypothesis posits that, on several occasions, ... thick sheet of ice lasting for millions of years. ...
Cached Biology News:Researchers could use plant's light switch to control cells 2Speed installation of system to monitor vital signs of global ocean, scientists urge 2Speed installation of system to monitor vital signs of global ocean, scientists urge 3Speed installation of system to monitor vital signs of global ocean, scientists urge 4Speed installation of system to monitor vital signs of global ocean, scientists urge 5Speed installation of system to monitor vital signs of global ocean, scientists urge 6Speed installation of system to monitor vital signs of global ocean, scientists urge 7Speed installation of system to monitor vital signs of global ocean, scientists urge 8Animal evolution springs from 'Snowball Earth' 2
...
Phospho-Btk (Tyr223) Antibody...
...
... to Ki67 - Proliferation Marker, prediluted ... related nuclear protein, expressed by proliferating cells ... cycle (G1, S, G2 and M phase). ... Ki67 antibodies are useful in establishing the ...
Biology Products: