Navigation Links
High-Throughput Cultivation of Bacterial Clones

XL1-Blue bacteria containing the pBluescript plasmid. One ml of LB medium, containing 100 mg/ml ampicillin, was added to each 2-ml well of the 96-well plate. Bacteria were inoculated into alternate wells of the plate at a density of 2 105 cells/ml. The plate was sealed with its flexible plastic lid, which is designed to seal each well to prevent cross contamination. Perforations were made in the lid over the first 48 wells to allow an exchange of gases between the air space above the culture medium and the atmosphere. The 96-deep-well plate was then incubated at 37C in the thermoshaker (600 rpm).

Bacteria at the same cell density were inoculated into a 2-liter flask, which held 1 liter of LB medium containing 100 g/ml ampicillin. The flask was covered with a piece of sterile aluminum foil and incubated in a New Brunswick Scientific Model G25 Series floor incubator-shaker at 300 rpm.

Bacterial growth was monitored at various time points by removing samples from the aerated and nonaerated wells of both the plate and flask, then measuring absorption at 550 nm.1 Bacteria cultured in nonaerated wells of the plate were removed by perforating the plate lid with a hot needle just prior to sampling. This method prevented the need to remove the lid from the plate and introduce air into the nonaerated wells of the plate.

Bacterial Growth Rates

Figure 2 demonstrates equivalent growth rates of bacteria in early- and mid-log phases in aerated and nonaerated wells of the plate. Late-log phase growth rates of bacteria cultured in nonaerated wells were only slightly slower than in aerated wells. Cell densities at the end of the incubation period in aerated and nonaerated wells were identical. Flask-cultured bacteria demonstrated a slower initial rate of growth compared to plate-cultu
'"/>

Source:


Page: All 1 2 3

Related biology technology :

1. Innovative Tissue Array Technology for High-Throughput Screening of Gene Expression
2. Highest Possible Transformation Efficiencies for High-Throughput Applications
3. High-Throughput Isolation of Total RNA
4. High-Throughput System Generates Ultra-Pure PCR Products Suitable for All Applications
5. The DIG System Nonradioactive Automated High-Throughput In Situ Hybridization: a Powerful Tool for Functional Genomics Research
6. Advantages of ReadyAgarose Precast Gels Over 96-Well Handcast Gels for High-Throughput Analysis, Rev A
7. Mammalian and Bacterial Expression in One Vector
8. Bacterial mRNA Isolation, Fast and Easy
9. Bacterial RNA Isolation From Infected Eukaryotic Hosts
10. Bacterial RNA Isolation From Infected Eukaryotic Hosts
11. Bacterial Whole Genome Array Analysis
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: High Throughput Cultivation Bacterial Clones

(Date:7/28/2014)... MADISON, Wis. , July 28, 2014   ... chronic respiratory disease, announced the hire of Brad ... Development. Towle will be responsible for driving the company,s ... "Brad,s broad experience in healthcare, and specifically his past ... valuable insight to the company at this pivotal time ...
(Date:7/28/2014)... 2014  InterMune, Inc. (NASDAQ: ITMN ) ... 2014 financial results at the close of the U.S. ... and webcast will be hosted by InterMune at 4:30 ... investors and others may participate in the conference call ... ID#21728775. A replay of the webcast and teleconference will be ...
(Date:7/26/2014)... Canada Endoscopy Visualization Systems Market ... the Canada Endoscopy Visualization Systems market. The report ... (in units) and average prices (in US dollars) ... Mid-Range Endoscopy Visualization Systems and Low-End Endoscopy Visualization ... . , The report also provides company shares ...
(Date:7/25/2014)... from the University of Leicester has furthered our ... potential to create new materials using nanosized ,building ... in the prestigious academic journal Physical Review ... examine in rich detail the structure and internal ... acetylene molecule and a single helium atom. , ...
Breaking Biology Technology:Propeller Health Hires Brad Towle as Senior Vice President of Business and Client Development 2InterMune to Release Second Quarter Financial Results on August 6 2Canada Endoscopy Visualization Systems Market Outlook to 2020: MarketResearchReports.Biz 2Canada Endoscopy Visualization Systems Market Outlook to 2020: MarketResearchReports.Biz 3Canada Endoscopy Visualization Systems Market Outlook to 2020: MarketResearchReports.Biz 4Canada Endoscopy Visualization Systems Market Outlook to 2020: MarketResearchReports.Biz 5Breakthrough laser experiment reveals liquid-like motion of atoms in an ultra-cold cluster 2Breakthrough laser experiment reveals liquid-like motion of atoms in an ultra-cold cluster 3
... 2008 Lentigen Corporation today,announced an exclusive ... for licensing technologies from the University of,Cambridge ... area of lentiviral vector,technology. The technology was ... at the University of Cambridge., "The ...
... Vice President of Finance -, HOPKINTON, Mass., April ... ) today announced the promotion of Peter F.,McAree to ... as Vice President of Finance at Caliper. Effective April,4, ... CFO., McAree has more than 20 years of ...
... Chairman and CEO Honors the Anniversary by Ringing The Closing Bell(R) ... ... Calif., April 3, 2008 Edwards,Lifesciences Corporation (NYSE: EW ), ... today announced its,50th anniversary of partnering with clinicians to develop life-saving,innovations. ...
Cached Biology Technology:Lentigen Licenses Fundamental Lentiviral Vector Technology Patent From the University of Cambridge 2Lentigen Licenses Fundamental Lentiviral Vector Technology Patent From the University of Cambridge 3Caliper Life Sciences Appoints Peter F. McAree Senior Vice President and Chief Financial Officer 2Edwards Lifesciences Celebrates 50 Years of Life-Saving Innovations 2Edwards Lifesciences Celebrates 50 Years of Life-Saving Innovations 3
(Date:7/25/2014)... factor characterized by the existence of numerous isoforms ... to the protein deeply different characteristics. NRG1 plays ... during development and the different phases occurring after ... regrowth, remyelination and target reinnervation, Researchers at the ... soluble NRG1 upregulation observed in Schwann cells immediately ...
(Date:7/25/2014)... PITTSBURGH -- The routine use of a molecular ... likelihood of performing the correct initial surgery for ... from the University of Pittsburgh Cancer Institute (UPCI), ... the UPMC/UPCI Multidisciplinary Thyroid Center and other diagnostic ... the correct initial surgery by 30 percent, according ...
(Date:7/24/2014)... Keryx Biopharmaceuticals, Inc. (Nasdaq:KERX) (the "Company") announced the publication ... 3 study of Zerenex (ferric citrate), the Company,s investigational ... hyperphosphatemia in patients with end-stage renal disease (ESRD) on ... with FErric CiTrate in EsrD) was published online today ... Nephrology ( JASN ). , This ...
Breaking Biology News(10 mins):Test increases odds of correct surgery for thyroid cancer patients 2Test increases odds of correct surgery for thyroid cancer patients 3Zerenex™ (ferric citrate) long-term Phase 3 study results published in JASN 2Zerenex™ (ferric citrate) long-term Phase 3 study results published in JASN 3Zerenex™ (ferric citrate) long-term Phase 3 study results published in JASN 4
... DALLAS May 20, 2008 Mutations in two ... the brain involved in sleep create a human-like insomnia ... found. The findings may help scientists better understand the ... This is one of the most dramatic ...
... exposure to chemicals is on the increase. From food ... with potentially-toxic substances could be affecting our health ... Nottingham are set to take part in one of ... on female mammals. Part of the Reproductive Effects of ...
... CITY Researchers have been unable to build an ... dream of ultrafast optical computers. But now, University of ... photonic crystals with the ideal, diamond-like structure: They are ... beetle from Brazil. It appears that a simple ...
Cached Biology News:Gene mutations in mice mimic human-like sleep disorder, UT Southwestern researchers find 2Do chemicals in the environment affect fertility? 2The photonic beetle 2The photonic beetle 3The photonic beetle 4The photonic beetle 5
... Rabbit polyclonal antibody to human XAB2 ... was first identified through its interaction with ... a fraction of XAB2 interacts with the ... well as with RNA polymerase II. Microinjection ...
... provide custom microplate coating using advanced automated high throughput microplate ... formats. We can also accommodate virtually any lot size of ... , 8 and 12 ... 384 well microplate, ...
...
Mynox is intended for research use only. Mynox is used for the elimination of Mycoplasma and Acholeplasma in cell and virus cultures, and other biologicals....
Biology Products: