Navigation Links
High-Throughput Cultivation of Bacterial Clones


Culture 192 clones simultaneously with the tempest thermoshaker

Jeff Braman
Stratagene

The tempest thermoshaker and a 96-deep-well round-bottom plate were used to cultivate bacterial clones in a high-throughput format. Early- and mid-log phase rates of bacterial growth were equivalent in aerated and nonaerated wells of the plate. Late-log phase growth rates of bacteria cultured in nonaerated wells were only slightly slower than growth rates measured in aerated wells. Final cell densities in aerated and nonaerated wells were identical. Flask-cultured bacteria demonstrated a slower initial rate of growth compared to plate-cultured bacteria, however, mid-log phase growth and final cell density were equivalent to plate-cultured bacteria. Well-to-well plate contamination was undetectable. Equal quantities of plasmid DNA were recovered from equal volumes of plate- and flask-cultured bacteria sampled at the end of the incubation period.

The Tempest thermoshaker is a compact benchtop incubator and shaker that is used for regulated temperature incubation (0 to 40C) and rotary mixing (200 to 1500 rpm) of multiple samples. The unit is supplied with a holder to accommodate fifty six 1.5-ml tubes. With Stratagenes minitube adapters, the thermoshaker can also hold 0.5- or 0.6-ml tubes. A microplate platform, also available, holds four, 96-well microtiter plates or two, 96-deep-well plates. The latter plates are particularly useful for cultivating bacteria in a high-throughput.

96-Well Plate vs. Flask Cultivation of Bacteria

Growth rates and final cell densities were measured for plate- and flask-cultured Epicurian Coli
'"/>

Source:


Page: All 1 2 3

Related biology technology :

1. Innovative Tissue Array Technology for High-Throughput Screening of Gene Expression
2. Highest Possible Transformation Efficiencies for High-Throughput Applications
3. High-Throughput Isolation of Total RNA
4. High-Throughput System Generates Ultra-Pure PCR Products Suitable for All Applications
5. The DIG System Nonradioactive Automated High-Throughput In Situ Hybridization: a Powerful Tool for Functional Genomics Research
6. Advantages of ReadyAgarose Precast Gels Over 96-Well Handcast Gels for High-Throughput Analysis, Rev A
7. Mammalian and Bacterial Expression in One Vector
8. Bacterial mRNA Isolation, Fast and Easy
9. Bacterial RNA Isolation From Infected Eukaryotic Hosts
10. Bacterial RNA Isolation From Infected Eukaryotic Hosts
11. Bacterial Whole Genome Array Analysis
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: High Throughput Cultivation Bacterial Clones

(Date:8/27/2014)... BOSTON , Aug. 27, 2014 Rhythm, ... diseases and genetic deficiencies that result in metabolic disorders, ... on Form S-1 with the U.S. Securities and Exchange ... of shares of its common stock. The number of ... the offering have not yet been determined. ...
(Date:8/27/2014)... plants survive impact by the huge amounts of ... this energy for photosynthesis. The hypothesis is that ... dissipate the energy throughout the entire protein molecule ... have now managed to successfully ,film, this process. ... proteins which play a role in photosynthesis and ...
(Date:8/27/2014)...  Lisa Kulik saw fireworks for the first time ... to a groundbreaking retinal implant co-invented by Mark ... the University of Southern California (USC) Eye Institute. ... resident who has retinitis pigmentosa, a degenerative ... June 2, she became the first person west of ...
(Date:8/27/2014)... , Aug. 27, 2014 Reportbuyer.com ... The Graphene Opportunity - A Rational View ... Graphene The New Nanotech? A similar amount ... of applications ranging from microelectronics to water treatment, ... announced the availability of the Graphene Opportunity Report, ...
Breaking Biology Technology:Rhythm Files Registration Statement for Proposed Initial Public Offering 2DTU researchers film protein quake for the first time 2USC Eye Institute ophthalmologists implant first FDA-approved Argus II retinal prosthesis in western United States 2USC Eye Institute ophthalmologists implant first FDA-approved Argus II retinal prosthesis in western United States 3USC Eye Institute ophthalmologists implant first FDA-approved Argus II retinal prosthesis in western United States 4USC Eye Institute ophthalmologists implant first FDA-approved Argus II retinal prosthesis in western United States 5The Graphene Opportunity - A Rational View 2The Graphene Opportunity - A Rational View 3The Graphene Opportunity - A Rational View 4
... MADISON At the UW-Madison College of Engineering, Dean ... ensuring that todays engineering education includes what it takes ... ,At the University of Wisconsin System and the Wisconsin ... how to graduate more people with four-year baccalaureate degrees ...
... MILWAUKEE Metavante Corporation and First ... a definitive agreement that provides for the purchase of ... by Metavante for approximately $610 million in cash. ... will receive approximately $389 million and NYCE will become ...
... In a column I wrote last year, I alluded to ... global. In fact, there are now 4,284 biotech companies in 25 ... , ,Moreover: , , The biotech industry has 622 publicly ... $16 billion in R&D in 2001. , , The industry ...
Cached Biology Technology:Wisconsin must produce more science-savvy students to remain competitive 2Wisconsin must produce more science-savvy students to remain competitive 3Metavante and First Data to buy PIN-debit company NYCE 2In Globalizing Biotech, Midwest Must Differentiate From Pack 2In Globalizing Biotech, Midwest Must Differentiate From Pack 3In Globalizing Biotech, Midwest Must Differentiate From Pack 4
(Date:8/27/2014)... Aug. 27, 2014  WaferGen Bio-systems, Inc. (NASDAQ: WGBS) ... underwritten public offering of units of common stock and ... gross proceeds of $20 million, prior to deducting underwriting ... Company.  The shares and warrants are immediately separable and ... sold in the offering consists of 2,000 shares of ...
(Date:8/27/2014)... the mysteries of the human genome was unveiled today ... most detailed comparison yet of how the genomes of ... The research, appearing August 28 in in the journal ... the three species, genomes is "read out," and how ... The results add billions of entries to a publicly ...
(Date:8/27/2014)... , Aug. 27, 2014 Reportlinker.com announces ... in its catalogue: Law Enforcement Biometrics ... http://www.reportlinker.com/p02316674/Law-Enforcement-Biometrics-Market-in-North-America-2014-2018.html About Biometrics Biometrics ... on the basis of their physical or behavioral ... both physiological traits such as fingerprints, face recognition, ...
Breaking Biology News(10 mins):WaferGen Bio-Systems Announces Successful Completion of Public Offering Raising $20 Million 2WaferGen Bio-Systems Announces Successful Completion of Public Offering Raising $20 Million 3Encyclopedia of how genomes function gets much bigger 2Encyclopedia of how genomes function gets much bigger 3Encyclopedia of how genomes function gets much bigger 4Law Enforcement Biometrics Market in North America 2014-2018 2Law Enforcement Biometrics Market in North America 2014-2018 3Law Enforcement Biometrics Market in North America 2014-2018 4Law Enforcement Biometrics Market in North America 2014-2018 5
... has identified a new genetic mutation responsible for osteogenesis imperfecta ... break, according to a study published today in the journal ... fractures in a lifetime or several hundred beginning before birth. ... from 20,000 to 50,000. While the study is an important ...
... combination of satellite data, computer models and weather balloon ... fires as much as doubled ground-level ozone thousands of ... July 2004. The study, which will appear online Sept. ... a rare glimpse at the precise air-quality effects from ...
... 1930s to estimate the abundance of fish has shown ... at reducing poaching of elephants, African buffaloes and black ... , "Wildlife within protected areas is under increasing threat ... argue that enforcement within protected areas is not sufficient ...
Cached Biology News:Researchers discover new gene responsible for brittle bone disease 2Researchers discover new gene responsible for brittle bone disease 3NASA study: Alaskan fires affected Houston air quality in 2004 2NASA study: Alaskan fires affected Houston air quality in 2004 3Serengeti patrols cut poaching of buffalo, elephants, rhinos 2
Each Yeast-GFP clone represents an individual S. cerevisiae strain containing an open reading frame with a C-terminal Aequorea Victoria GFP (S65T) fusion tag....
... is a new product number, created ... If showing no availability yet, please ... (R4276) or contact customer service for ... surface, easy grip) Mfr ...
... are stored within neurons in vesicles ... in the synaptic bouton. Synaptosomes ... from other cellular debris. Abnormalites ... in many pathological condtions. Conditions ...
... 4200A can be upgraded to the GenePix ... scans, analyzes, and saves results for up ... email when the batch is complete. Line-by-line ... warped slides. GenePix Pro gives you complete ...
Biology Products: