Navigation Links
High Quality RNA Isolation Services


High quality RNA from tissue or cells
Only ethically sourced tissue is used
Custom RNA isolations from tissue
Optimized isolation protocols


Quality Begins at the Source
Use Ambion's Custom Isolation Services and be assured that you are getting the highest quality RNA from ethically sourced donor tissue with all available clinical information . Our total RNA is purified using methods that allow for microRNA recovery, which is often lost using traditional methods.

As part of our standard quality control for custom RNA, all samples are tested for nuclease activity, shown to contain less than 0.05% DNA, and checked for stability via overnight incubation at 37C. Figure 2 illustrates this point: Rat thymus RNA was stored in suboptimal conditions (4C) and analyzed after two extended time points (1 month and 7 months). Both the electropherogram profile of the RNA and ribosomal RNA (rRNA) ratios indicated minimal degradation suggesting that the RNA was free from RNase contamination. Also, the rRNA ratios for both time points were between 1.6 and 2.0, indicating high quality, intact RNA.


Ethical Sourcing and Extensive Donor Information
Access to high quality RNA and DNA from human tissues is important for researchers involved in gene expression studies. Furthermore, these samples must be isolated from ethically sourced tissue. Ambion's Custom Isolation Services Group works with a large network of partners to provide you with both high quality products and ethical tissue procurement. When you utilize Ambion's isolation services, you also receive key information critical to your analysis--sample donor history and diagnoses. Figure 1 demonstrates the information detail available to our customers.

For more information about custom RNA isolation, send an email to custom@ambion.com.

Figure 1. Donor Information for Human Breast tumor RNA.

Figure 2. Rat Thymus RNA Stability Test. 8-10 week old rat thymus total RNA was isolated according to standardized in-house protocols. Aliquots of the RNA were then stored at 4C for extended periods of time. 100 ng of the RNA was assessed on an Agilent 2100 bioanalyzer after (A) 1 month at 4C and (B) 7 months at 4C. Both scans indicate high quality RNA that is stable at suboptimal storage conditions.


back to top


'"/>

Source:


Page: All 1 2

Related biology technology :

1. A Simple and Rapid Technique to Purify High-Quality Midiprep DNA
2. Generate High-Quality, Directional Plasmid cDNA Libraries
3. Quality control of antibodies using the 2100 bioanalyzer and the Protein 200 Plus assay
4. Quality assurance of RNA derived from laser microdissected tissue samples obtained by the PALM MicroBeam System using the RNA 6000 Pico LabChip kit.
5. Isolate High Quality Total RNA from LCM Samples Suitable for Microarray and qRT-PCR Analysis
6. Preparation of PCR Quality Template DNA from a Wide Range of Tissues Using InstaGene Matrix
7. Fast and Easy Isolation of PCR-Ready Genomic DNA from Whole Blood
8. High-Throughput Isolation of Total RNA
9. Simple Isolation of RNA from Tissue and Cultured Cells
10. Fast Isolation of Total RNA from Small Numbers of Cells
11. Comparison of Growth Techniques and Media for the Purpose of Plasmid Isolation from E. coli Using the Eppendorf Perfectprep Plasmid Mini Kit
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:3/27/2017)... Md. , March 27, 2017  The ... billion for 2016, according to a new report ... medical lab testing is performed to evaluate disease ... individual therapy, among other reasons.  The healthcare market ... Market , provides an overview of the ...
(Date:3/27/2017)... (PRWEB) , ... March 27, 2017 , ... ... method to engineer scalable and customizable vascular grafts in JoVE’s Video Journal, the ... lead to new and improved ways of treating coronary artery disease (CAD). Lam ...
(Date:3/24/2017)... March 24, 2017 MiMedx Group, Inc. (NASDAQ: ... human placental tissue allografts and patent-protected processes to develop ... of healthcare, announced today  that it will present at ... , NY.  Parker H. "Pete" Petit, Chairman and ... Christopher M. Cashman , EVP and Chief Commercialization ...
(Date:3/24/2017)... 2017 On Thursday, March 23, ... 5,817.69, down 0.07%; the Dow Jones Industrial Average edged ... 500 closed at 2,345.96, marginally dropping 0.11%. US markets ... green, 4 sectors finished in red, and 1 sector ... initiated reports coverage on the following Biotechnology equities: BioDelivery ...
Breaking Biology Technology:
(Date:3/23/2017)... 23, 2017 Research and Markets has announced ... & Trends - Industry Forecast to 2025" report to their ... The ... CAGR of around 8.8% over the next decade to reach approximately ... the market estimates and forecasts for all the given segments on ...
(Date:3/22/2017)... 2017 Optimove , provider of ... such as 1-800-Flowers and AdoreMe, today announced two ... Replenishment. Using Optimove,s machine learning algorithms, these features ... replenishment recommendations to their customers based not just ... customer intent drawn from a complex web of ...
(Date:3/20/2017)... At this year,s CeBIT Chancellor Dr. Angela Merkel ... came to the DERMALOG stand together with the Japanese Prime Minster Shinzo ... At the largest German biometrics company the two government leaders could see ... as well as DERMALOG´s multi-biometrics system.   Continue ... ...
Breaking Biology News(10 mins):