Navigation Links
High Quality RNA Isolation Services


High quality RNA from tissue or cells
Only ethically sourced tissue is used
Custom RNA isolations from tissue
Optimized isolation protocols


Quality Begins at the Source
Use Ambion's Custom Isolation Services and be assured that you are getting the highest quality RNA from ethically sourced donor tissue with all available clinical information . Our total RNA is purified using methods that allow for microRNA recovery, which is often lost using traditional methods.

As part of our standard quality control for custom RNA, all samples are tested for nuclease activity, shown to contain less than 0.05% DNA, and checked for stability via overnight incubation at 37C. Figure 2 illustrates this point: Rat thymus RNA was stored in suboptimal conditions (4C) and analyzed after two extended time points (1 month and 7 months). Both the electropherogram profile of the RNA and ribosomal RNA (rRNA) ratios indicated minimal degradation suggesting that the RNA was free from RNase contamination. Also, the rRNA ratios for both time points were between 1.6 and 2.0, indicating high quality, intact RNA.


Ethical Sourcing and Extensive Donor Information
Access to high quality RNA and DNA from human tissues is important for researchers involved in gene expression studies. Furthermore, these samples must be isolated from ethically sourced tissue. Ambion's Custom Isolation Services Group works with a large network of partners to provide you with both high quality products and ethical tissue procurement. When you utilize Ambion's isolation services, you also receive key information critical to your analysis--sample donor history and diagnoses. Figure 1 demonstrates the information detail available to our customers.

For more information about custom RNA isolation, send an email to custom@ambion.com.

Figure 1. Donor Information for Human Breast tumor RNA.

Figure 2. Rat Thymus RNA Stability Test. 8-10 week old rat thymus total RNA was isolated according to standardized in-house protocols. Aliquots of the RNA were then stored at 4C for extended periods of time. 100 ng of the RNA was assessed on an Agilent 2100 bioanalyzer after (A) 1 month at 4C and (B) 7 months at 4C. Both scans indicate high quality RNA that is stable at suboptimal storage conditions.


back to top


'"/>

Source:


Page: All 1 2

Related biology technology :

1. A Simple and Rapid Technique to Purify High-Quality Midiprep DNA
2. Generate High-Quality, Directional Plasmid cDNA Libraries
3. Quality control of antibodies using the 2100 bioanalyzer and the Protein 200 Plus assay
4. Quality assurance of RNA derived from laser microdissected tissue samples obtained by the PALM MicroBeam System using the RNA 6000 Pico LabChip kit.
5. Isolate High Quality Total RNA from LCM Samples Suitable for Microarray and qRT-PCR Analysis
6. Preparation of PCR Quality Template DNA from a Wide Range of Tissues Using InstaGene Matrix
7. Fast and Easy Isolation of PCR-Ready Genomic DNA from Whole Blood
8. High-Throughput Isolation of Total RNA
9. Simple Isolation of RNA from Tissue and Cultured Cells
10. Fast Isolation of Total RNA from Small Numbers of Cells
11. Comparison of Growth Techniques and Media for the Purpose of Plasmid Isolation from E. coli Using the Eppendorf Perfectprep Plasmid Mini Kit
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:10/12/2017)... ... October 12, 2017 , ... The Blavatnik Family ... and six Finalists of the 2017 Blavatnik Regional Awards for Young Scientists. ... and administered by the New York Academy of Sciences to honor the excellence ...
(Date:10/12/2017)... ... October 12, 2017 , ... BioMedGPS announces expanded ... of its newest module, US Hemostats & Sealants. , SmartTRAK’s US Market for ... fibrin sealants, synthetic sealants and biologic sealants used in surgical applications. BioMedGPS estimates ...
(Date:10/11/2017)... , ... October 11, 2017 , ... ... President Andi Purple announced Dr. Suneel I. Sheikh, the co-founder, CEO and ... Labs ), Inc. has been selected for membership in ARCS Alumni Hall ...
(Date:10/11/2017)... Alto, CA, USA (PRWEB) , ... October 11, 2017 , ... ... set to take place on 7th and 8th June 2018 in San Francisco, CA. ... policy influencers as well as several distinguished CEOs, board directors and government officials from ...
Breaking Biology Technology:
(Date:4/13/2017)... , April 13, 2017 UBM,s Advanced Design ... will feature emerging and evolving technology through its ... Summits will run alongside the expo portion of the ... panels and demonstrations focused on trending topics within 3D ... design and manufacturing event will take place June 13-15, 2017 ...
(Date:4/11/2017)... , April 11, 2017 Crossmatch®, a ... authentication solutions, today announced that it has been ... Research Projects Activity (IARPA) to develop next-generation Presentation ... "Innovation has been a driving force ... program will allow us to innovate and develop ...
(Date:4/11/2017)... N.Y. , April 11, 2017 ... fingerprints, but researchers at the New York University ... College of Engineering have found that partial similarities ... security systems used in mobile phones and other ... thought. The vulnerability lies in the ...
Breaking Biology News(10 mins):