Quality Begins at the Source
Use Ambion's Custom Isolation Services and be assured that you are getting the highest quality RNA from ethically sourced donor tissue with all available clinical information . Our total RNA is purified using methods that allow for microRNA recovery, which is often lost using traditional methods.
As part of our standard quality control for custom RNA, all samples are tested for nuclease activity, shown to contain less than 0.05% DNA, and checked for stability via overnight incubation at 37C. Figure 2 illustrates this point: Rat thymus RNA was stored in suboptimal conditions (4C) and analyzed after two extended time points (1 month and 7 months). Both the electropherogram profile of the RNA and ribosomal RNA (rRNA) ratios indicated minimal degradation suggesting that the RNA was free from RNase contamination. Also, the rRNA ratios for both time points were between 1.6 and 2.0, indicating high quality, intact RNA.
Ethical Sourcing and Extensive Donor Information
Access to high quality RNA and DNA from human tissues is important for researchers involved in gene expression studies. Furthermore, these samples must be isolated from ethically sourced tissue. Ambion's Custom Isolation Services Group works with a large network of partners to provide you with both high quality products and ethical tissue procurement. When you utilize Ambion's isolation services, you also receive key information critical to your analysis--sample donor history and diagnoses. Figure 1 demonstrates the information detail available to our customers.
For more information about custom RNA isolation, send an email to firstname.lastname@example.org.
Figure 1. Donor Information for Human Breast tumor RNA.
Figure 2. Rat Thymus RNA Stability Test. 8-10 week old rat thymus total RNA was isolated according to standardized in-house protocols. Aliquots of the RNA were then stored at 4C for extended periods of time. 100 ng of the RNA was assessed on an Agilent 2100 bioanalyzer after (A) 1 month at 4C and (B) 7 months at 4C. Both scans indicate high quality RNA that is stable at suboptimal storage conditions.