Navigation Links
High-Level Expression of Peanut Allergens Affected by Rare Codon Usage


Produce large quantities of difficult-to-express proteins

Tamara Kleber-Janke Wolf-Meinhard Becker
Biochemical & Molecular Allergology, Research Center Borstel, Germany

By using Stratagenes BL21-CodonPlus(DE3)-RIL cells * with extra copies of E. coli, argU, ileY, and leuW tRNA genes, it is possible to attain high-level expression of difficult proteins affected by poor codon usage: IPTG-induced expression of several recombinant peanut allergen genes, such as Ara h 2, were greatly increased in these special cells compared to the expression yield achieved by conventional E. coli cells like M15 or BL21-Gold(DE3) cells.

E. coli remains a popular host for the expression of heterologous proteins. Although the E. coli cell has a tremendous capacity to produce large quantities of protein, there are limits when the composition of the mRNA or protein is not typical. Within E. coli, a clear bias exists among the 61 amino acid codons found within the population of mRNA molecules, and the level of cognate tRNA appears directly proportional to the frequency of codon usage.1

A subset of codons, namely AGG/AGA, AUA, CUA, CGA, and CCC, are the least used codons in E. coli that are encoded by rare tRNAs. Recent studies suggest that an excess of any of these codons creates problems during translation, leading to a reduction in quantity of the protein synthesized.2 Errors are detectable as frameshifts and decreased levels of expression. More subtle changes, such as mistranslation, may also be occurring.

Until recently, three alternatives were usually recommended to solve these problems: Use a host containing a plasmid with the appropriate tRNA,3 synthesize the gene to replace rare codons with frequently used codons, and use aeukaryotic expression system.

Comparing Different Expression Vectors and E. coli Strains


'"/>

Source:


Page: All 1 2 3 4

Related biology technology :

1. New Mammalian Expression Vectors Employ Stable, High-Level Fluorescence Humanized Renilla GFP Reporter
2. High-Level Protein Expression, One-Column Purification, and FLAG Epitope Tagging in E. coli
3. An Epitope Tagging Vector for Gene Expression in Mammalian Cells
4. Antibodies for Studying NMDA Receptor Protein Expression and Synapse-Specific Immunolabeling
5. Versatile Vectors for Ponasterone A- Inducible Control of Gene Expression in Mammalian Cells
6. Innovative Tissue Array Technology for High-Throughput Screening of Gene Expression
7. Functional Cloning Using ViraPort Retroviral cDNA Expression Libraries
8. A New Lambda Vector for Mammalian Expression
9. Expression and Purification of Recombinant Proteins That Have Native Amino Acid Sequence
10. Codon Bias-Adjusted BL21 Derivatives for Protein Expression
11. Tools for Detecting MSH2 Expression in Chinese Hamster Ovary Cells
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: High Level Expression Peanut Allergens Affected Rare Codon Usage

(Date:1/22/2015)... CO / Rootstown, OH (PRWEB) January 22, 2015 ... detection platform, announced today that it has received AOAC-PTM Certifications ... O45, O1O3, O111, O121, and O145; collectively referred to as ... coli) O157, at 1 colony forming unit (cfu) per 325 ...
(Date:1/22/2015)... 22, 2015 Selexis SA, a serial ... Cell Banks (RCBs) used for drug discovery to commercial ... will include Next-Generation Sequencing (NGS) data packages. ... biologic manufacturing by ensuring the integrity of the gene, ...
(Date:1/22/2015)...   GenoSpace , a precision medicine software company that has ... use of genomic, imaging and other biomedical data in research ... Munson , CEO of Aspera, an IBM Company, to its ... http://photos.prnewswire.com/prnh/20150122/170713 "We are pleased to welcome ...
(Date:12/24/2014)... 24, 2014  Vermillion, Inc. (Nasdaq:   VRML), a ... today that the Company closed its previously announced ... Jack W. Schuler , Birchview Fund LLC ... million, before offering expenses.  The proceeds will be ...
Breaking Biology Technology:Crystal Diagnostics Awarded AOAC-PTM Accreditation for the Rapid Detection of “Big 6” E.coli Food Pathogens 2Selexis Generated Research Cell Banks Now Fully Sequenced Using Next-Generation Sequencing 2GenoSpace Expands Board with Appointment of Michelle Munson 2Vermillion Announces Closing of Equity Financing 2Vermillion Announces Closing of Equity Financing 3
... no yellow tape or chalk outlines of a body, but ... want remedies, said a lawyer who spoke at the ... a partner with Michael Best & Friedrich , said ... or disgruntled employees can benefit from learning to work with ...
... a warning for chief information officers: By 2007, 80 percent ... executives. The CEO of Paragon Development Systems , an ... to stay involved with the business side of their companies ... change in a really short period of time," he said. ...
... If, five years ago, you asked a group of ... have said storage capacity, security or bandwidth. , ,Today, ... spate of securities legislation, highlighted by Congress's passage of ... has come to be called. SOX, which was passed ...
Cached Biology Technology:After a successful attack on your computer systems, then what? 2After a successful attack on your computer systems, then what? 3IT outsourcing remains on the horizon as companies focus on core business 2IT outsourcing remains on the horizon as companies focus on core business 3With Sarbanes-Oxley, compliance takes center stage for CIOs 2With Sarbanes-Oxley, compliance takes center stage for CIOs 3
(Date:12/19/2014)... , Dec. 18, 2014 Research and Markets ( ... Market Monitor: North America Perimeter Security Systems Market" report ... The North American perimeter security market is estimated ... 2019. Although the U.S. market holds a larger share in ...
(Date:12/17/2014)... NEW YORK , Dec. 15, 2014  HITLAB SM ... Practices (GCP) audit to confirm its adherence to current ... enables HITLAB to conduct regulated smart device and smart ... safety and research quality. "HITLAB is determined ...
(Date:12/11/2014)... Research and Markets , ... announced the addition of the "Biometrics Market in ... One major trend emerging in this market ... it is important to upgrade biometric solutions to the ...
Breaking Biology News(10 mins):Micro Market Monitor: North America Perimeter Security Systems Market (Intrusion Detection Sensor, Video Surveillance, Communication/Alarm and Notification, Access Control System) 2Micro Market Monitor: North America Perimeter Security Systems Market (Intrusion Detection Sensor, Video Surveillance, Communication/Alarm and Notification, Access Control System) 3HITLAB Completes GCP Audit for FDA Smart Device & App Compliance 2Biometrics Market in the APAC Region 2015-2019: Key Vendors are 3M Cogent, NEC, Safran and Suprema 2
... entomologists are set to meet next week at the ... as the North Central Branch of the Entomological Society ... topics will include public health/bed bug entomology, biocontrol of ... and Sontrol tactics, soybean aphid management, and insect biodiversity ...
... -- It,s a cloak that surpasses all others: a ... the way bacteria and other cells are imaged. ... State University, and his research team are wrapping bacteria ... under electron microscopes. Berry,s method creates a carbon cloak ...
... , This release is available in Spanish ... grass breeder has rediscovered a forage grass that seems just ... of an unusual forage grass led Michael Casler, an Agricultural ... Research Center in Madison, Wis., to identify the grass as ...
Cached Biology News:All wrapped up: K-State researcher's graphene cloak protects bacteria, leading to better images 2All wrapped up: K-State researcher's graphene cloak protects bacteria, leading to better images 3Dairy farmer finds unusual forage grass 2
... of BAC (100+kb), fosmid (40kb), and large ... than any othe BAC vector. On command ... and easy recombinant DNA purification. Available with ... VC) vectors for the highest insert stability. ...
... cloning of BAC (100+kb), fosmid (40kb), and ... stability than any othe BAC vector. On ... yields and easy recombinant DNA purification. Available ... (pSMART VC) vectors for the highest insert ...
RABBIT ANTI GLYCINE...
Request Info...
Biology Products: