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High-Fidelity PCR with a Novel Polymerase Mixture

New polymerase mixture, TaqPlus Precision PCR system, developed for highfidelity PCR


Kirk B. Nielson * Janice Cline * Frances Bai * Daniel McMullan * Barbara McGowan * Holly Hogrefe
Stratagene Cloning Systems, Inc.


Stratagene announces the addition of the TaqPlus Precision PCR system ,* to an expanding line of PCR products. The TaqPlus Precision PCR system consists of a novel mixture of Taq2000 and Pfu DNA polymerases and a reaction buffer that has been developed for high-fidelity PCR applications. As with many two-enzyme PCR mixtures, the TaqPlus Precision PCR system tends to generate higher yields of PCR product than can be obtained using single-enzyme formulations.

With the introduction of Taq DNA polymerase in 1987,1 the polymerase chain reaction (PCR) amplification of DNA target regions has become an important and widely used tool of molecular biology. Since then, many additional thermophilic DNA polymerases and polymerase mixtures have been developed for use in PCR amplifications. Stratagene now offers four different thermophilic polymerases or polymerase mixtures for PCR: Taq DNA polymerase, Pfu DNA polymerase,** the TaqPlus Long PCR system,## (formerly TaqPlus DNA polymerase) and most recently, the TaqPlus Precision PCR system. All of these polymerases and polymerase systems are licensed for PCR, and each offers distinct characteristics and advantages.

Taq and Taq2000 DNA Polymerase for Reliability and Speed

Taq DNA polymerase has been the traditional workhorse enzyme of PCR amplification research. This enzyme was isolated originally from the thermophilic bacterium Thermus aquaticus strain YT1. Taq DNA polymerase exhibits both thermostable 5' - to 3'- polymerase and 5 - to 3 - exonuclease activities but lacks an associated 3 - to 5 - exonuclease-dependent proofreading activity. The
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