Navigation Links
High Fidelity PCR Master

for amplification of up to 5 kb targets from genomic DNA Cat. No. 2 140 314 200 reactions Description Premixed, 2x concentrated High Fidelity PCR Master contains all necessary reagents (buffers, nucleotides and enzymes) needed for PCR. The mix contains a unique enzyme blend of Taq DNA Polymerase and Pwo DNA Polymerase[a thermostable enzyme with a proofreading (3'5' exonuclease) activity]. Due to the inherent 3'5' exonuclease proofreading activity of Tgo DNA Polymerase, the High Fidelity PCR Master increases the fidelity of DNA Synthesis approx. threefold compared to Taq DNA Polymerase. The High Fidelity PCR Master generates blunt-ended products and 3' single A-overhang products analogous to Taq DNA Polymerase. Note: Separate vials of PCR grade sterile water are included with the High Fidelity PCR Master Application
  • High Fidelity PCR Master is ideal for amplifying up to 5 kb targets from genomic DNA
  • High Fidelity PCR Master gives better results (higher yield and greater fidelity) than Taq DNA Polymerase alone in standard PCR reactions
  • High Fidelity PCR Master can be used instead of the Expand High Fidelity PCR System, or instead of single reagents
Operating parameters
  • Starting sample: up to 500 ng genomic or 10 ng plasmid DNA.
  • Temperature optimum (for elongation):
    72C (for <3 kb targets)
    68C (for >3 kb targets)
  • High Fidelity PCR Master accepts modified nucleotides like DIG-dUTP
Key advantages
  • Improves fidelity of PCR amplification, because High Fidelity PCR Master amplifies with threefold greater accuracy than Taq DNA Polymerase alone
  • Gives better results with impure templates, because High Fidelity PCR Master is less affected by contaminants in a DNA preparation than other DNA polymerases
  • Saves time, because High Fidelity PCR Master will amplify even long templates without requiring adjustment of reaction buffer components
  • Works with low amounts of template, because High Fidelity Master is more sensitive than other DNA polymerases
  • Convenient storage at 4C means that the High Fidelity PCR Master does not need to be thawed; it can be used immediately
  • Convenient, because the PCR reaction mix is 2x concentrated and ready-to-use


'"/>

Source:


Page: All 1 2

Related biology technology :

1. Comparing Fidelity and Performance of Proofreading PCR Enzymes
2. prostar RT-PCR Systems for Robust High-Fidelity RNA Amplification
3. pfuturbo DNA Polymerase: A High-Performance, High-Fidelity Enzyme Ideal for PCR Cloning
4. High-Fidelity PCR with a Novel Polymerase Mixture
5. Unique Enhanced DNA Polymerase Delivers High Fidelity and Great PCR Performance
6. Expand High FidelityPLUS PCR System
7. Expand High Fidelity PCR System
8. Challenge the Performance of Your Hot-Start PCRs with FastStart Taq DNA Polymerase and the Novel FastStart High Fidelity PCR System
9. Combine High Yield, Great Accuracy and the Prevention of Carry-over Contamination by Using the Novel Expand High FidelityPLUS PCR System
10. TripleMaster PCR System
11. TripleMaster PCR System
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:6/27/2016)... ... 2016 , ... Parallel 6 , the leading software as a service ... Virtual Patient Encounter CONSULT module which enables both audio and video telemedicine communication ... , Using the CONSULT module, patients and physicians can schedule a face to face ...
(Date:6/24/2016)... -- Regular discussions on a range of subjects including policies, debt ... said Poloz. Speaking at a lecture to the ... pointed to the country,s inflation target, which is set by ... "In certain areas there needs to ... goals, why not sit down and address strategy together?" ...
(Date:6/23/2016)... WI (PRWEB) , ... June 23, 2016 , ... ... supplements, is pleased to announce the launch of their brand, UP4™ Probiotics, into ... for over 35 years, is proud to add Target to its list of ...
(Date:6/23/2016)... ... June 23, 2016 , ... ... (Yeast and Mold) microbial test has received AOAC Research Institute approval 061601. , ... tests introduced last year,” stated Bob Salter, Vice President of Regulatory and Industrial ...
Breaking Biology Technology:
(Date:5/9/2016)... , UAE, May 9, 2016 ... it comes to expanding freedom for high net worth ... Even in today,s globally connected world, there is still ... system could ever duplicate sealing your deal with a ... second passports by taking advantage of citizenship via investment ...
(Date:4/26/2016)... and LONDON , April ... part of EdgeVerve Systems, a product subsidiary of ... today announced a partnership to integrate the Onegini ...      (Logo: http://photos.prnewswire.com/prnh/20151104/283829LOGO ) ... their customers enhanced security to access and transact ...
(Date:4/15/2016)... April 15, 2016 Research ... Gait Biometrics Market 2016-2020,"  report to their offering.  ... ) , ,The global gait biometrics market is ... during the period 2016-2020. Gait analysis ... can be used to compute factors that are ...
Breaking Biology News(10 mins):