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High Electrotransformation Efficiencies Obtained With DNA From Ligation Mixtures

low DNA concentrations may result in loss of precious material. Adding glycogen as a carrier helps to reduce this problem. Ethanol precipitation is also considered by some people to be a relatively time-consuming step in the preparation of DNA for electroporation. I conclude that the easiest and quickest method for achieving high electroporation efficiencies is to heat-treat the ligation reaction, followed by a 1/100 dilution. Only a fraction of the ligation reactions are used for the dilutions, leaving the remaining DNA for other purposes.


References
1. Dower, W. J., In Genetic Engineering-Principles and Methods, Setlow, J. K., ed, 1990, vol. 12, pp. 275 - 296, Plenum Press, New York.

2. Willson, T. A. and Gough, N. M., Nucl. Acids Res. 16, 11820 (1988).

3. Zabarovsky, E. R. and Winberg, G., Nucl. Acids Res. 18, 5912 (1990).

4. Jacobs, M., Wnendt, S. and Stahl, U., Nucl. Acids Res. 18, 1653 (1990).

5. Ymer, S., Nucl. Acids Res. 19, 6960 (1991).


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