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High-Efficiency Transfections Achieved with New Low-Toxicity Reagent

lines, GeneJammer reagent demonstrated a high transfection efficiency, low toxicity, and the highest number of viable transfectants.

Transfection of Primary Cells

One important measure of transfection reagents is how efficient they operate in cells that are resistant to transfection, such as primary cells. Typically, primary cells are less robust than transformed cells, yet the need for transfection of these types of cells is high. Successful transfection of primary cells allows studies in the more physiologically relevant biological models. Immortalized or transformed cell lines often have undefined phenotypes, and these phenotypes must be extensively characterized.

We transfected the pCMVbgal reporter plasmid into HUVEC and HUAEC cells, which are primary cells, with GeneJammer reagent or each of five competitors transfection reagents and compared the results. The best outcomes were obtained in serum-free media. The transfections were analyzed according to these parameters: transfection efficiency, total viable cells, and viable transfectants. Results (Figure 5) illustrate the high performance of GeneJammer reagent, confirming its capacity for efficient transfection in less-than-robust and difficult-to-transfect cells.

Conclusions

Use GeneJammer reagent, a polyamine proprietary formulation, to realize high transfection efficiency and low cytotoxicity when inserting DNA into cells. After comparing GeneJammer and five other leading transfection reagents, we found that GeneJammer reagent works well in the presence or absence of serum and, hence, is ideal to use when varying transfection conditions are desired. Moreover, GeneJammer reagent transfected the HUVEC and HUAEC primary cell lines with high efficiency, indicating its c
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