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High-Efficiency Transfections Achieved with New Low-Toxicity Reagent


Nonliposomal formulation yields high efficiencies in a variety of transfection conditions

Brenda Rogers Paul Kotturi Natalia Novoradovskaya
Stratagene

Introducing foreign DNA into eukaryotic cells requires high-potency transfections, which demand superior quality transfection reagents that are efficient, have low toxicity, and work in the presence or absence of serum. We compared Stratagenes new GeneJammer transfection reagent to each of five leading transfection reagents. GeneJammer reagent, a new nonliposomal formulation, not only performed well in numerous cell lines, it also yielded high efficiencies in primary cells. When the viability of transfected cells was evaluated, GeneJammer reagent showed the highest number of living transfectants for all cell lines tested.

The demand for high-efficiency transfections is increasing, reflecting the expanding applications for transfection technology. Introducing foreign DNA into eukaryotic cells has become fundamental to analyzing gene function, producing recombinant gene products, and devising strategies for gene therapy. Successful transfection results rely on the transfection method and the capability of the transfection reagent.1,2,3 The ideal transfection reagent is characterized by specific attributes, such as high efficiency in a wide variety of cells in the presence or absence of serum and the inclusion of antibiotics, growth factors, or other supplements. Other important attributes include low toxicity, high stability, and easy handling.

Stratagenes new GeneJammer transfection reagent, which is a proprietary formulation of a polyamine and other components, offers these essential qualitites for achieving successful transfection results. We subjected GeneJammer reagent and five of the most popular transfection reagents to extensive analyses, comparing transfection perform
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