Navigation Links
High-Efficiency Transfections Achieved with New Low-Toxicity Reagent


Nonliposomal formulation yields high efficiencies in a variety of transfection conditions

Brenda Rogers Paul Kotturi Natalia Novoradovskaya
Stratagene

Introducing foreign DNA into eukaryotic cells requires high-potency transfections, which demand superior quality transfection reagents that are efficient, have low toxicity, and work in the presence or absence of serum. We compared Stratagenes new GeneJammer transfection reagent to each of five leading transfection reagents. GeneJammer reagent, a new nonliposomal formulation, not only performed well in numerous cell lines, it also yielded high efficiencies in primary cells. When the viability of transfected cells was evaluated, GeneJammer reagent showed the highest number of living transfectants for all cell lines tested.

The demand for high-efficiency transfections is increasing, reflecting the expanding applications for transfection technology. Introducing foreign DNA into eukaryotic cells has become fundamental to analyzing gene function, producing recombinant gene products, and devising strategies for gene therapy. Successful transfection results rely on the transfection method and the capability of the transfection reagent.1,2,3 The ideal transfection reagent is characterized by specific attributes, such as high efficiency in a wide variety of cells in the presence or absence of serum and the inclusion of antibiotics, growth factors, or other supplements. Other important attributes include low toxicity, high stability, and easy handling.

Stratagenes new GeneJammer transfection reagent, which is a proprietary formulation of a polyamine and other components, offers these essential qualitites for achieving successful transfection results. We subjected GeneJammer reagent and five of the most popular transfection reagents to extensive analyses, comparing transfection perform
'"/>

Source:


Page: All 1 2 3 4 5

Related biology technology :

1. High-Efficiency Site-Directed Mutagenesis of Large Plasmid Vectors
2. Streamline Your siRNA Transfections
3. 140-Fold Increase of Protein Achieved in Pichia Fermentation
4. Low-Toxicity, Lipid-Mediated Transfection of Mammalian Cells
5. Brilliant Core Reagent Kits for QPCR and QRT-PCR
6. A Comparison Study of Lipid Tranfection Reagents in A549, NIH 3T3 and COS-7 Cell Lines
7. Versatile Transfection Reagent Offers Low Toxicity and Consistent Performance
8. Protocol for RNA Isolation Using TRIzol Reagent with Phase Lock Gel-Heavy
9. Protocol for RNA Isolation Using TRIzol Reagent with Phase Lock Gel-Heavy
10. TransMessenger Transfection Reagent
11. Effectene Transfection Reagent provides efficient gene delivery to primary neuronal cell cultures
Post Your Comments:
(Date:7/11/2014)... July 11, 2014 /CNW/ - Sunovion Pharmaceuticals Canada Inc. ... (eslicarbazepine acetate) for use as a once-daily adjunctive ... with epilepsy who are not satisfactorily controlled with conventional ... in patients under 18 years of age. ... disorders and according to Epilepsy Canada, it affects 0.6% ...
(Date:7/10/2014)... 2014 Women in the West ... an additional family planning option. Sayana® Press has ... all levels of the health system and in ... widely used contraceptive—Pfizer’s Depo-Provera® (depot medroxyprogesterone acetate)—with the ... the first of four African countries expected to ...
(Date:7/10/2014)... Understanding the need for better quality and higher ... that currently exists in this area, ChanTest has recently ... validated ion channel and GPCR cell lines. ChanTest’s ... channels control many critical physiological functions throughout the body. ... to treat a variety of human diseases. , ...
(Date:7/10/2014)... Robert Harman, DVM, Founder and CEO of Vet-Stem, Inc., ... the relaunch of his highly informative blog, now named Stem ... What are Stem Cells ?” Dr. Harman’s purpose for ... the basics of stem cell therapy so that pet owners ... treatment when considering regenerative medicine. , A veterinarian by trade, ...
Breaking Biology Technology:Sunovion Pharmaceuticals Canada Inc. Announces Health Canada Approval of APTIOM(TM) (eslicarbazepine acetate) as a Once-Daily Adjunctive Treatment for Partial-Onset Seizures in Patients with Epilepsy 2Sunovion Pharmaceuticals Canada Inc. Announces Health Canada Approval of APTIOM(TM) (eslicarbazepine acetate) as a Once-Daily Adjunctive Treatment for Partial-Onset Seizures in Patients with Epilepsy 3Sunovion Pharmaceuticals Canada Inc. Announces Health Canada Approval of APTIOM(TM) (eslicarbazepine acetate) as a Once-Daily Adjunctive Treatment for Partial-Onset Seizures in Patients with Epilepsy 4Injectable Contraceptive Launched in Burkina Faso to Expand Choice and Address Unmet Need 2Injectable Contraceptive Launched in Burkina Faso to Expand Choice and Address Unmet Need 3Injectable Contraceptive Launched in Burkina Faso to Expand Choice and Address Unmet Need 4Injectable Contraceptive Launched in Burkina Faso to Expand Choice and Address Unmet Need 5Injectable Contraceptive Launched in Burkina Faso to Expand Choice and Address Unmet Need 6Leader in Ion Channel Research Announces Newly Validated and Re-Engineered Cells 2Leader in Ion Channel Research Announces Newly Validated and Re-Engineered Cells 3Robert Harman, DVM Talks About What Stem Cells are in His Latest Blog Series for Vet-Stem, Inc. 2Robert Harman, DVM Talks About What Stem Cells are in His Latest Blog Series for Vet-Stem, Inc. 3
... Fuel Cell Technology On The Ground in Los Angeles and planned for New ... ... ... Virgin Atlantic, one of,the world,s leading long-haul airlines, and General Motors, the world,s,largest automaker, ...
... Company,s Leading microRNA Intellectual ... Property Position ... Allowed Patents, and More Than 60 Pending Patent ... Rosetta Genomics, Ltd. (NASDQ: ROSG) announced,today that the U.S. Patent and ...
... Anatomic Pathology LIS Interface to Digital Pathology Information ... ... March 3 IMPAC Medical,Systems, Inc. (IMPAC), an Elekta company ... laboratories,and Aperio Technologies, (Aperio) a global leader in digital pathology ...
Cached Biology Technology:Virgin Atlantic to Offer Zero-Emissions GM Hydrogen Fuel Cell Limousines to Upper Class Passengers 2Virgin Atlantic to Offer Zero-Emissions GM Hydrogen Fuel Cell Limousines to Upper Class Passengers 3Rosetta Genomics Announces Allowance of Two Additional microRNA Patents 2Rosetta Genomics Announces Allowance of Two Additional microRNA Patents 3Rosetta Genomics Announces Allowance of Two Additional microRNA Patents 4IMPAC Medical Systems and Aperio to Develop Integrated Solution for Anatomic Pathology 2IMPAC Medical Systems and Aperio to Develop Integrated Solution for Anatomic Pathology 3
(Date:7/11/2014)... Standards and Technology (NIST) need a special tool ... prove that necessity is truly the mother of ... Lorna De Leoz and Stephen Stein, NIST chemists ... is the study of the abundant, often-branched sugar ... and lipids and influence cellular processes, including immunity, ...
(Date:7/11/2014)... An international team of researchers, including scientists from the ... just a reported a major step in understanding photosynthesis, ... now maintains the oxygen in its atmosphere and which ... on earth. , The researchers report the first ... reaction, namely the step in which a specific protein ...
(Date:7/11/2014)... transplantation of mesenchymal stem cells can stimulate neurogenesis in ... Alzheimer,s disease (AD) and improve tissue and function injury ... reported on the therapeutic effect of adipose-derived stem cells ... effect on oxidative injury and neurogenesis in the brain ... School of Life Sciences, Tsinghua University, China transplanted ADSCs ...
Breaking Biology News(10 mins):A first direct glimpse of photosynthesis in action 2
... To most people in the southwestern U.S., the April 4 ... But on a group of inch-long fish that exist nowhere ... the ground in Nevada,s Mojave Desert, it unleashed a veritable ... the event on cameras installed above and below the water,s ...
... (EPA) has awarded nearly $2.5 million to 11 ... environmental areas: monitoring and control of air emissions, ... monitoring, water infrastructure, homeland security, nanotechnology and innovation ... of concept" awards from EPA, and will use ...
... JOLLA, Calif., May 11, 2010 -- Investigators from around ... on Friday, May 7, to share their knowledge of ... non-coding nucleic acids turn off proteins and have been ... numerous other conditions. "The discovery that small ...
Cached Biology News:'Tsunami' video sheds light on struggling pupfish 2'Tsunami' video sheds light on struggling pupfish 3'Tsunami' video sheds light on struggling pupfish 4'Tsunami' video sheds light on struggling pupfish 5EPA awards small businesses to develop new environmental technologies 2Researchers share insights into RNA 2
MOUSE ANTI RAT KAPPA/LAMBDA:HRP Immunogen: MARK-1: Rat kappa light chains (IR202 and IR968)MARL-15: Rat lambda light chains (RH58, IR31)...
Bovine Serum Albumin Peroxidase Stabilizer Solution, 1 L...
Component of GELase™ Agarose Gel-Digesting Preparation. 1 ml of 50X Buffer is sufficient for replacing the buffer in at least 5 g of gel....
...
Biology Products: