Navigation Links
High-Efficiency Site-Directed Mutagenesis of Large Plasmid Vectors

ransformation, which are important when mutagenizing large templates. The QuikChange XL kit retains the simplicity and ease-of-use of the parent kit, while ensuring high-efficiency mutagenesis of a broad range of plasmid DNA templates.

REFERENCES
  1. Kunkel, T.A. (1985) Proc. Nat. Acad. Sci. USA 82: 488-492.
  2. Bohnsack, R.N. (1996) In Vitro Mutagenesis Protocols 57: 1-12.
  3. Zhu, L. (1996) In Vitro Mutagenesis Protocols 57: 13-29.
  4. Papworth, C., et al. (1996) Strategies 9: 3-4.
  5. Hogrefe, H., et al. (1997) Strategies 10: 93-96.
  6. Jerpseth, A., et al. (1997) Strategies 10: 37-38.

* U.S. Patent Nos. 5,789,166 and 5,932,419 and patents pending
** U.S. Patent Nos. 5,512,468 and 5,707,841 and patents pending
*** U.S. Patent No. 5,545,552 and 5,866,395 and 5,948,663 and patents pending


'"/>

Source:


Page: All 1 2 3 4

Related biology technology :

1. High-Efficiency Transfections Achieved with New Low-Toxicity Reagent
2. Big Blue Rodent Cell Cultures for Mutagenesis Studies
3. Randomize Gene Sequences with New PCR Mutagenesis Kit
4. PCR-Grade Nucleotides Larger Pack Sizes Now Available
5. Rapidly Synthesize Large Amounts of Capped RNA
6. Efficient Cloning and Electro-transformation of Large Eukaryotic DNA Fragments
7. Sub-Micron Gold Particles Are Superior to Larger Particles for Efficient Biolistic Transformation of Organelles and Some Cells
8. Efficiently Insert Unique Restriction Sites into Plasmid Vectors
9. Simple Purification of DNA from Plasmid Minipreps, PCR Amplifications and Agarose Gels
10. Generate High-Quality, Directional Plasmid cDNA Libraries
11. New Reporter Plasmids for Studying Interferon-Stimulated Signal Transduction Pathways
Post Your Comments:
(Date:10/7/2008)...ected to aid drug discovery by allowing for the ra...gle reaction, ST. LOUIS, Oct. 7 /PRNewswire-First...o announce that it has signed an exclusive licensi...bana-Champaign allowing,Sigma-Aldrich to offer res...ogates for use in Suzuki-Miyaura coupling to resea...
(Date:10/7/2008)...s to focus on new patient data supporting the Ross...ynerGraft processed human heart .../- CryoLife, Inc. (NYSE: C...ocessing company, announced today,that cardiovascu...e in the,Ross Summit 2008, a two-day surgical cong...
(Date:10/7/2008)...Institutes of Health, The Infectious Disease Resea...Launch and Board of Advisors, SEATTLE, Oct. 7 /PR...,Initiative today announced its first acquisition ...osis (TB) drug candidates. Agreements were reached...nd the Microbial Chemistry,Research Foundation (MC...
(Date:10/7/2008)... Oct. 7 /- BioEnergy International, L...r and manufacturer of,next-generation, renewable b...f Norman R. Augustine to its Board of Advisors eff...f distinguished leadership and innovation,at the h...g in his role as,Chairman and Chief Executive Offi...
Breaking Biology Technology:Sigma-Aldrich and the University of Illinois Offer New Boronic Acid Surrogates to Researchers Worldwide Through Licensing Agreement 2Sigma-Aldrich and the University of Illinois Offer New Boronic Acid Surrogates to Researchers Worldwide Through Licensing Agreement 3CryoLife Hosts Worldwide Surgical Congress for the Ross Procedure 2CryoLife Hosts Worldwide Surgical Congress for the Ross Procedure 3CryoLife Hosts Worldwide Surgical Congress for the Ross Procedure 4Update: The Lilly TB Drug Discovery Initiative Begins Work on New Compounds to Fill Early-Stage TB Drug Pipeline 2Update: The Lilly TB Drug Discovery Initiative Begins Work on New Compounds to Fill Early-Stage TB Drug Pipeline 3Update: The Lilly TB Drug Discovery Initiative Begins Work on New Compounds to Fill Early-Stage TB Drug Pipeline 4Update: The Lilly TB Drug Discovery Initiative Begins Work on New Compounds to Fill Early-Stage TB Drug Pipeline 5BioEnergy International, LLC Announces Norman R. Augustine to Join Advisory Board 2BioEnergy International, LLC Announces Norman R. Augustine to Join Advisory Board 3
...A law firm here has teamed up with an IT consultin...ations affecting the health care industry. , , Rei... Cyberlynk Networks, Inc., of Franklin are offeri...mpanies to comply with security requirements of th...ct (HIPAA). , ,The HIPAA security rule requires co...
... an operating group of Brookfield, Wis.-based Fise...lan marketplace with an administration and financi...Through a multi-year agreement with EDS, Fiserv H...ance Application and hosting services, aimed at he...is new solution to provide greater insights into b...
.... -- Criticare Systems, Inc. has received FDA a..., the company announced Tuesday. It will start mar...r. , ,The system is intended to monitor physiologi...nvironment. Its design allows a variety of optiona...t clinical settings around the world. , ,Criticare...
... - The X-ray development firm Bruker AXS announ...s, France firm that produces X-ray materials resea... close early in the first quarter of 2006. , ,The ...ing to Bruker,s 8-K form just filed with the US Se...er will acquire all shares of SOCABIM,s capital st...
Other Biology Technology:Bruker acquires French X-ray software company 2
(Date:10/6/2008)...ammal Assessment project results are announced thi...lona, Spain, there will be at least seven Texas sp...cies of mammals from Texas that are classified as ...egories are commonly known as Mexican long-tongued...st cottontail, desert pocket gopher, Texas kangaro...
(Date:10/6/2008)...ugene Woltering, the James D. Rives Professor of S...nter New Orleans School of Medicine, and Dr. Conra...have been awarded a US Patent for a compound to in...he growth of cancerous tumors and diseases like di...olipoprotein A-I is the major protein component of...
(Date:10/6/2008)...ber 6, 2008) A gene contained in laboratory yeast...ncover new findings about the process by which pro...in order to initiate gene expression, according to...eviously thought to be inert carriers of the genet...s turn out to reveal a novel mechanism for creatin...
(Date:10/6/2008)...ber and November 2008, some 150 scientists from 40...sts from the U.S. Department of Energy,s (DOE) Bro... international field experiment designed to make o...system of the southeastern Pacific. Because elemen... represented in global climate models, collecting ...
Breaking Biology News(10 mins):7 Texas mammals listed as threatened on Global Mammal Assessment 2LSUHSC awarded patent for compound inhibiting cancer and other diseases 2Early-stage gene transcription creates access to DNA 2Brookhaven scientists take off for southeastern Pacific climate study 2Optometry Backed Vision Care for Kids Act Approved by U S House 3752 1Optometry Backed Vision Care for Kids Act Approved by U S House 3752 2Optometry Backed Vision Care for Kids Act Approved by U S House 3752 3Optometry Backed Vision Care for Kids Act Approved by U S House 3752 4National Alliance for Hispanic Health Ramps Up Lobbying for SCHIP Veto Override 3749 1National Alliance for Hispanic Health Ramps Up Lobbying for SCHIP Veto Override 3749 2Pacific Valley Bank Approves 2416 7 Million Loan to Mee Memorial Hospital for Healthcare Services in South Monterey County 3745 1Pacific Valley Bank Approves 2416 7 Million Loan to Mee Memorial Hospital for Healthcare Services in South Monterey County 3745 2Pacific Valley Bank Approves 2416 7 Million Loan to Mee Memorial Hospital for Healthcare Services in South Monterey County 3745 3Veto of Californias Condoms in Prisons Bill 3A 22Politics Over Public Health 22 Says AHF 522 1Veto of Californias Condoms in Prisons Bill 3A 22Politics Over Public Health 22 Says AHF 522 2Veto of Californias Condoms in Prisons Bill 3A 22Politics Over Public Health 22 Says AHF 522 3
...s better when it comes to body size, since many li...evolved into larger species over time. , But a st...niversity of California, San Diego in an early onl...demy of Sciences suggests that maxim, known as "Co...tists found that populations of tiny crustaceans r...
...for a new home, usually a tree cavity, they have a...th great efficiency they narrow down the options a...e, says Cornell University biologist Thomas Seeley...ops. , The Seeley group,s study, which is publish...ght well be used to help improve human group decis...
...pores ?the all-important channels that control the...s ?double in number to prepare for the split to co...cientists at the Salk Institute for Biological Stu...rmed from scratch, and inserted themselves into th...picture of how a cell divides in such a way that t...
... cancer virus causes infected immune cells to clin...ead. , The virus, the human T lymphotropic virus ...ed cells known as T lymphocytes, or T cells, touch... how this cell-to-cell transmission happens. It su...es infected T cells and causes them to become stic...
Other Biology News:Study finds evolution doesn't always favor bigger animals 2Study finds evolution doesn't always favor bigger animals 3Honeybee decision-making ability rivals any department committee 2Honeybee decision-making ability rivals any department committee 3Researchers solve mystery of how nuclear pores duplicate before cell division 2Viral protein helps infected T cells stick to uninfected cells 2
Compound(s) tested through 63 ligand binding and enzyme assays spanning a broad therapeutic range. This assay panel includes many non human tissue sources and several non-selective assays. Used to d
...evolutionizes DNA sequencing, delivering sequence ...Sequencer 20 System includes: Instrument and acc...struction, amplification, and sequencing Analysis...instrument is the centerpiece of the Genome Sequen...
Compound(s) tested through a broad panel of 38 ligand binding and enzyme assays targeting the Central Nervous System. Used to determine selectivity and potential CV/Renal liabilities of compounds.
...• Human serum is obtained from normal human d...uman Immunodeficiency Virus Antigen (s) (HIV-1Ag),...HIV 1/2), Hepatitis C Virus (HCV), and Nonreactive...eactive by a screening test for syphilis. Serum is...
Biology Products: