Navigation Links
High-Efficiency Site-Directed Mutagenesis of Large Plasmid Vectors


QuikChange XL: a highly versatile kit for site-directed mutagenesis of large plasmids

Michael Borns Peter Vaillancourt Holly Hogrefe Jeff Braman
Stratagene

The QuikChange site-directed mutagenesis kit* characteristically generates mutagenesis efficiencies exceeding 80%. We have created a new kit for mutagenesis of large plasmid templates. The QuikChange XL site-directed mutagenesis kit has been specifically formulated for mutagenesis of longer targets, and is packaged with XL10-Gold ultra competent cells**, which are optimized for the transformation of larger DNA molecules. We demonstrate the improved performance of the QuikChangeXL kit by mutagenizing a 10.1-kb retroviral vector that was otherwise difficult to mutagenize.

Site-directed mutagenesis is routinely employed in numerous research endeavors. The procedure can be laborious and time-consuming, requiring either single-stranded DNA templates1, subcloning into specialized vectors2, plasmid vectors containing unique restriction sites, and/or multiple transformations into different host strains3. In contrast, the QuikChange site-directed mutagenesis kit4 features a highly efficient procedure that is significantly streamlined when compared to other mutagenesis methods. With this kit, site-specific mutation can take place in virtually any double-stranded plasmid, eliminating the need for subcloning into M13-based bacteriophage vectors and ssDNA rescue. In addition, the kits procedure does not require specialized vectors, unique restriction sites, or multiple transformations. The protocol can be completed in just one day and generates mutagenesis efficiencies exceeding 80%.

Another important feature of the kit is the use of high-fidelity PfuTurbo DNA polymerase.*** This key component permits template DNA replicatio
'"/>

Source:


Page: All 1 2 3 4

Related biology technology :

1. High-Efficiency Transfections Achieved with New Low-Toxicity Reagent
2. Big Blue Rodent Cell Cultures for Mutagenesis Studies
3. Randomize Gene Sequences with New PCR Mutagenesis Kit
4. PCR-Grade Nucleotides Larger Pack Sizes Now Available
5. Rapidly Synthesize Large Amounts of Capped RNA
6. Efficient Cloning and Electro-transformation of Large Eukaryotic DNA Fragments
7. Sub-Micron Gold Particles Are Superior to Larger Particles for Efficient Biolistic Transformation of Organelles and Some Cells
8. Efficiently Insert Unique Restriction Sites into Plasmid Vectors
9. Simple Purification of DNA from Plasmid Minipreps, PCR Amplifications and Agarose Gels
10. Generate High-Quality, Directional Plasmid cDNA Libraries
11. New Reporter Plasmids for Studying Interferon-Stimulated Signal Transduction Pathways

Post Your Comments:
(Date:11/9/2009)...O, Nov. 9 NexBio, Inc. announced t...rican College of Allergy, Asthma, and Immunology i...DAS181 (Fludase®), a Sialidase, Decreases Air...fects in Animal Models of Asthma". ,, DAS181 (F...um host-targeting drug candidate being studied in ...
(Date:11/8/2009)...2009 As global temperatures and energy costs cont...key to a sustainable future. An attractive repla... published online in Genome Research ( www.genom...tures of bioethanol-producing microorganisms, unco...loping new technologies needed to implement produc...
(Date:11/6/2009)...Ontario, Nov. 6 Helix Bi...F) today announced that John Docherty, president a...an Curhan Ford,s 6th annual Investor Summit on Nov...New York City. Mr. Docherty will provide an overv...rograms L-DOS47 and Topical Interferon Alpha-2b. ...
(Date:11/6/2009)... Nov. 6 /PRNewswire-FirstCall/ - Oncolytics Biotec... "Company") today announced updated results from a...ents with sarcomas metastatic to the lung in a pos...sue Oncology Society Meeting held in Miami Beach, ...entation, entitled "A Phase II Study of Intravenou...
Breaking Biology Technology:NexBio(R) Presents Data Showing Anti-Asthma Activity of DAS181 (Fludase(R)*) at ACAAI 2009 2NexBio(R) Presents Data Showing Anti-Asthma Activity of DAS181 (Fludase(R)*) at ACAAI 2009 3Genomes of biofuel yeasts reveal clues that could boost fuel ethanol production worldwide 2Helix BioPharma to Present at Merriman Curhan Ford's Investor Summit 2009 on November 10th 2Helix BioPharma to Present at Merriman Curhan Ford's Investor Summit 2009 on November 10th 3Oncolytics Biotech(R) Inc. Collaborators Present Positive Phase II Sarcoma Trial Results at CTOS Annual Meeting 2Oncolytics Biotech(R) Inc. Collaborators Present Positive Phase II Sarcoma Trial Results at CTOS Annual Meeting 3
... transplantation studies , hNT BlueNeurons Expr...hael McGrogan * Guadalupe Gonzalez * Waldemar Lern...a , , Stratagene,s hNT BlueNeurons express bet...rom a clone of the NT2 teratocarcinoma stably tran...use they are easily distinguished from,surroundi...
...lt heterologous proteins , Carsten-Peter Car...as created variants of the Epicurian Coli ,BL2...series** of strains, which contain extra copies of...is modification allows for a high-level,expressi...nventional E. coli,hosts due to the codon bias o...
...as little as 50 ng of RNA , Barry R. Neiditc...,John C. Bauer Mike Kobrin Quinn Lu Dianne Ec...ng ,Human Genome Sciences, Rockville, Maryland ,...uction Kit, which,introduces a new method to gen...,vector. Use this kit with either enriched poly(A...
Other Biology Technology:hNT BlueNeurons Express b-Galactosidase at High ,Levels 2hNT BlueNeurons Express b-Galactosidase at High ,Levels 3hNT BlueNeurons Express b-Galactosidase at High ,Levels 4hNT BlueNeurons Express b-Galactosidase at High ,Levels 5Codon Bias-Adjusted BL21 Derivatives for Protein Expression 2Codon Bias-Adjusted BL21 Derivatives for Protein Expression 3Codon Bias-Adjusted BL21 Derivatives for Protein Expression 4Codon Bias-Adjusted BL21 Derivatives for Protein Expression 5Codon Bias-Adjusted BL21 Derivatives for Protein Expression 6Codon Bias-Adjusted BL21 Derivatives for Protein Expression 7Constructing Directional cDNA Libraries from Limited Amounts of RNA 2Constructing Directional cDNA Libraries from Limited Amounts of RNA 3Constructing Directional cDNA Libraries from Limited Amounts of RNA 4Constructing Directional cDNA Libraries from Limited Amounts of RNA 5Constructing Directional cDNA Libraries from Limited Amounts of RNA 6Constructing Directional cDNA Libraries from Limited Amounts of RNA 7
(Date:11/9/2009)...government and industry researchers has completed ...enome. The project is an important first step in a...ce crop and addressing some of the many limitation...Cassava is a root crop that serves as the primary ...day. Although it has many properties that make it ...
(Date:11/9/2009)...., Nov. 9 Genencor, a division of ...Award" from the American Institute of Chemical Eng... enzymes for cellulosic ethanol. Yesterday, Robert...nted the award to Landon Steele, who is Marketing ...oduct Manager, and Aaron Kelley, who is Senior Eng...
(Date:11/9/2009)... responsible for the rapid, mysterious death of ph...ound by collaborating scientists at Rutgers Univer...(WHOI). This same chemical may hold unexpected pr...a previously unknown lipid, or fatty compound, in ...ania huxleyi , a phytoplankton that plays a major ...
Breaking Biology News(10 mins):Researchers complete draft genome sequence for cassava 2Researchers complete draft genome sequence for cassava 3Researchers complete draft genome sequence for cassava 4Genencor Wins The American Institute of Chemical Engineers (AIChE) Sustainable Energy Award For Accellerase(R) Cellulosic Ethanol Enzymes 2Newly discovered fat molecule: An undersea killer with an upside 2Newly discovered fat molecule: An undersea killer with an upside 3Newly discovered fat molecule: An undersea killer with an upside 4Tobacco Control Bill Heads to White House as Health Groups Cheer 48777 1Tobacco Control Bill Heads to White House as Health Groups Cheer 48777 2Tobacco Control Bill Heads to White House as Health Groups Cheer 48777 3No Time for Complacency in Pandemic Planning 3A Aon 48774 1No Time for Complacency in Pandemic Planning 3A Aon 48774 2No Time for Complacency in Pandemic Planning 3A Aon 48774 3New Studies Show Nursing Workforce Critical to Success of Health Reform 48770 1New Studies Show Nursing Workforce Critical to Success of Health Reform 48770 2New Studies Show Nursing Workforce Critical to Success of Health Reform 48770 3New Studies Show Nursing Workforce Critical to Success of Health Reform 48770 4New Studies Show Nursing Workforce Critical to Success of Health Reform 48770 5New Studies Show Nursing Workforce Critical to Success of Health Reform 48770 6
...IDENCE, R.I. [Brown University] Brown University ...hat could someday help fight the aging process. , ... and others adds another piece to the puzzle that ..., cell biology and biochemistry, first discovered ...e Indy ("I,m Not Dead Yet") gene that can extend t...
...ST LAFAYETTE, Ind. - Researchers have created a pr...tentially many other biological molecules by using...ubes anchored to gold-coated "nanocubes." , The d...h tetherball is a sensor and is anchored to electr... tether and ultrathin wire to conduct electrical s...
... LANSING, Mich. A patented Michigan State Univers...version into ethanol means extra nutrients don,t h...ls from cellulose. , The AFEX (ammonia fiber expa...le, University Distinguished Professor of chemical...o make the breakdown of cellulose and hemicellulos...
Other Biology News:Scientists unlock possible aging secret in genetically altered fruit fly 2Nano-tetherball biosensor precisely detects glucose 2Nano-tetherball biosensor precisely detects glucose 3Nano-tetherball biosensor precisely detects glucose 4Process can cut the cost of making cellulosic biofuels 2
...O 9001:2000 Certified USDA Research Registered OLA...ed, SOP's Training AALAS Certified Technicians ...Documentation Upon Request Focus CRP is your one...y service needs. We offer free project consultati...
...XL provides balance data collection and automation...integration with Microsoft Excel , Pre-configured...isture analyzers , New balances can be added and c...le balances into one spreadsheet , Allows for mu...
ReBlot Plus Kit from CHEMICON
...ing age, the autofluorescent pigment lipofuscin ac...luding neurons. The presence of lipofuscin granule...y in the central nervous system because of its bro... with those of most commonly used fluorophores. Th...
Biology Products: