Navigation Links
HiSpeed Plasmid Kits

For ultrafast purification of up to 750 g plasmid or cosmid DNA

Features and benefits
  • Less than 45 min (Midi) or 60 min (Maxi) from harvested culture to ultrapure DNA
  • Lysate clearing and isopropanol precipitation without centrifugation
  • No risk of DNA pellet loss during precipitation
  • Up to 200 g (Midi) or 750 g (Maxi) yield of high-copy plasmid DNA


* Actual yields depend on plasmid copy number, size of insert, host strain, culture medium, and culture volume.
Culture volumes depend on plasmid copy number, size of insert, host strain, and culture medium.

Principle

QIAfilter Cartridges are special filter units designed to replace the centrifugation step following alkaline lysis of bacterial cells. QIAfilter Cartridges completely remove SDS precipitates and clear bacterial lysates in a fraction of the time needed for centrifugation.
HiSpeed Tips have an improved design which allows a very high flow rate, permitting DNA binding, washing, and elution steps for plasmid purification to proceed faster. In addition, the new anion-exchange resin contained in HiSpeed Tips has an increased capacity, allowing yields of up to 200 g high-copy plasmid to be obtained from HiSpeed Midi Tips, and up to 750 g high-copy plasmid to be obtained from HiSpeed Maxi Tips.
The unique QIAprecipitator module replaces the centrifugation step traditionally used to collect isopropanol-precipitated DNA following purification. The QIAprecipitator module traps the precipitated DNA while the isopropanolbuffer mixture flows through. The DNA is then simply eluted from the QIAprecipitator into a microcentrifuge tube with TE buffer or water. This unique module also eliminates the risk of pellet loss, which can occur during decanting of the supernatant following centrifugation.


Procedure

Neutralized bacterial lysates are incubated in the QIAfilter Cartridge and cleared in seconds by filtration. The filtrate is applied to a HiSpeed Tip for plasmid DNA purification. Eluted DNA is mixed with isopropanol and applied to the QIAprecipitator module using the syringe provided. The concentrated and desalted DNA is then eluted from the QIAprecipitator directly into a microcentrifuge tube with TE buffer or water.

Applications

Ultrapure DNA purified with HiSpeed Plasmid Kits yields excellent results in all applications, from cloning and sequencing to transfection.





'"/>

Source:


Page: All 1 2

Related biology technology :

1. Efficiently Insert Unique Restriction Sites into Plasmid Vectors
2. High-Efficiency Site-Directed Mutagenesis of Large Plasmid Vectors
3. Simple Purification of DNA from Plasmid Minipreps, PCR Amplifications and Agarose Gels
4. Generate High-Quality, Directional Plasmid cDNA Libraries
5. New Reporter Plasmids for Studying Interferon-Stimulated Signal Transduction Pathways
6. New Fusion Trans-Activator Plasmids for Studying Signal Transduction Pathways
7. Comparison of Growth Techniques and Media for the Purpose of Plasmid Isolation from E. coli Using the Eppendorf Perfectprep Plasmid Mini Kit
8. Basic Plasmid DNA Isolation Protocol
9. RNA-free Plasmid DNA Isolation Protocol
10. Basic Plasmid DNA Isolation Protocol
11. RNA-free Plasmid DNA Isolation Protocol
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:4/22/2020)... WEST CHESTER, Ohio (PRWEB) , ... April 21, ... ... availability of Biophen™ FXIII chromogenic method for determination of FXIII. , Aniara Diagnostica, ... has strengthened its commitment to meeting today’s highest standards for product quality and ...
(Date:4/7/2020)... ... April 06, 2020 , ... Gateway Genomics , leading developer of direct-to-consumer ... Early Gender DNA Tests sold in March, just months after attaining the 150,000-test ... product, where a mom could order her kit online, collect a DNA sample from ...
(Date:4/1/2020)... Texas (PRWEB) , ... March 30, 2020 , ... ... (APMD) and telehealth developer Reliant Immune Diagnostics (RID), formed earlier this year to ... COVID-19 pandemic by creating in-home tests for COVID-19. Company officials are currently?awaiting an ...
(Date:4/1/2020)... ... March 31, 2020 , ... Bedoukian Research, Inc. ... Joint Development Agreement that builds on each company’s expertise in developing efficient routes ... the supply of high-quality insect pheromones as well as flavors and fragrances. P2 ...
Breaking Biology Technology:
(Date:5/28/2020)... ... May 28, 2020 , ... DuPont ... liquefaction: AMYLEX® 6T. The innovative enzyme enables numerous business gains for breweries ... locally-sourced raw materials, improve productivity, maximize yield, ensure consistent quality and ultimately ...
(Date:5/25/2020)... , ... May 22, 2020 , ... Oxford Nanopore Technologies ... detection of SARS-CoV-2, the virus that causes COVID-19. , The LamPORE assay ... of smaller sample numbers, to very large numbers of samples. Hardware and reagents ...
(Date:5/21/2020)... ... May 20, 2020 , ... Catalent, the leading global ... and gene therapies, and consumer health products, today announced that Jean-François Chaubard, Senior ... the ISCT 2020 Paris Virtual conference, which takes place on 28 – 29 ...
Breaking Biology News(10 mins):