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HiSpeed Plasmid Kits

For ultrafast purification of up to 750 g plasmid or cosmid DNA

Features and benefits
  • Less than 45 min (Midi) or 60 min (Maxi) from harvested culture to ultrapure DNA
  • Lysate clearing and isopropanol precipitation without centrifugation
  • No risk of DNA pellet loss during precipitation
  • Up to 200 g (Midi) or 750 g (Maxi) yield of high-copy plasmid DNA


* Actual yields depend on plasmid copy number, size of insert, host strain, culture medium, and culture volume.
Culture volumes depend on plasmid copy number, size of insert, host strain, and culture medium.

Principle

QIAfilter Cartridges are special filter units designed to replace the centrifugation step following alkaline lysis of bacterial cells. QIAfilter Cartridges completely remove SDS precipitates and clear bacterial lysates in a fraction of the time needed for centrifugation.
HiSpeed Tips have an improved design which allows a very high flow rate, permitting DNA binding, washing, and elution steps for plasmid purification to proceed faster. In addition, the new anion-exchange resin contained in HiSpeed Tips has an increased capacity, allowing yields of up to 200 g high-copy plasmid to be obtained from HiSpeed Midi Tips, and up to 750 g high-copy plasmid to be obtained from HiSpeed Maxi Tips.
The unique QIAprecipitator module replaces the centrifugation step traditionally used to collect isopropanol-precipitated DNA following purification. The QIAprecipitator module traps the precipitated DNA while the isopropanolbuffer mixture flows through. The DNA is then simply eluted from the QIAprecipitator into a microcentrifuge tube with TE buffer or water. This unique module also eliminates the risk of pellet loss, which can occur during decanting of the supernatant following centrifugation.


Procedure

Neutralized bacterial lysates are incubated in the QIAfilter Cartridge and cleared in seconds by filtration. The filtrate is applied to a HiSpeed Tip for plasmid DNA purification. Eluted DNA is mixed with isopropanol and applied to the QIAprecipitator module using the syringe provided. The concentrated and desalted DNA is then eluted from the QIAprecipitator directly into a microcentrifuge tube with TE buffer or water.

Applications

Ultrapure DNA purified with HiSpeed Plasmid Kits yields excellent results in all applications, from cloning and sequencing to transfection.





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