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HiSpeed Plasmid Kits

For ultrafast purification of up to 750 g plasmid or cosmid DNA

Features and benefits
  • Less than 45 min (Midi) or 60 min (Maxi) from harvested culture to ultrapure DNA
  • Lysate clearing and isopropanol precipitation without centrifugation
  • No risk of DNA pellet loss during precipitation
  • Up to 200 g (Midi) or 750 g (Maxi) yield of high-copy plasmid DNA


* Actual yields depend on plasmid copy number, size of insert, host strain, culture medium, and culture volume.
Culture volumes depend on plasmid copy number, size of insert, host strain, and culture medium.

Principle

QIAfilter Cartridges are special filter units designed to replace the centrifugation step following alkaline lysis of bacterial cells. QIAfilter Cartridges completely remove SDS precipitates and clear bacterial lysates in a fraction of the time needed for centrifugation.
HiSpeed Tips have an improved design which allows a very high flow rate, permitting DNA binding, washing, and elution steps for plasmid purification to proceed faster. In addition, the new anion-exchange resin contained in HiSpeed Tips has an increased capacity, allowing yields of up to 200 g high-copy plasmid to be obtained from HiSpeed Midi Tips, and up to 750 g high-copy plasmid to be obtained from HiSpeed Maxi Tips.
The unique QIAprecipitator module replaces the
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Related biology technology :

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11. RNA-free Plasmid DNA Isolation Protocol
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