Navigation Links
Heteroduplex Analysis Using the DCode System

rate the same as the wild-type. Under these conditions, it was not possible to resolve the two homoduplex bands of the ΔF508 mutant. This mutation may be detected if heteroduplexes are formed by mixing the mutant and wild-type samples together, then denaturing and renaturing the DNA.

The ability to resolve the heteroduplex bands in the mutant samples makes it possible to distinguish between the mutant and wild-type samples. Heteroduplex Analysis can be used as a method for screening heterozygous mutations in the cystic fibrosis gene using the DCode system.


References
1. Nagamine, C.M., Chan, K. and Lau, Y-F.C., Hum. Genet., 45, 337-339 (1989).

2. White, M., Carvalho, M., Derse, D., OBrien, S. and Dean, M., Genomics, 12, 301-306 (1992).


*The Polymerase Chain Reaction (PCR) process is covered by patents owned by Hoffmann-LaRoche. Use of the PCR process requires a license.


back to top
'"/>

Source:


Page: All 1 2 3

Related biology technology :

1. A Positive Selection Assay for Mutation Analysis in Big Blue Animals
2. Epitope-Tagging Vectors for Functional Analysis in Yeast
3. Map and Link Human Genetic Disorders with SSLP Analysis
4. CastAway Precast Gels for Rapid Automated DNA Sequence Analysis
5. Single-cell RT-PCR Analysis of Paramecium Primaurelia with the Eppendorf cMaster RTplusPCR System
6. Structural Analysis of Glycosylated Peptides in Complex Mixtures with Ion Trap MSn
7. Use of Transcriptor Reverse Transcriptase in Microarray Analysis
8. Increased Sensitivity in Microarray Analysis
9. Gene-Expression Analysis of TP, DPD, and TS Using the LightCycler mRNA Quantification KitsPLUS
10. Agilent 2100 Bioanalyzer Automated Analysis System
11. Analysis of Microarray Data
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Heteroduplex Analysis Using the DCode System

(Date:1/15/2014)... Jan. 15, 2014 TaiGen Biotechnology Company, Limited ("TaiGen") ... with R-Pharm, a leading Russian pharmaceutical company, to develop ... Russian Federation , Turkey ... (CIS). Nemonoxacin is a novel antibiotic for the treatment of ...
(Date:1/14/2014)... Histogen, Inc., a regenerative medicine company developing ... simulated embryonic conditions, today announced that they have entered ... for physician-dispensed aesthetic products containing Histogen’s proprietary multipotent cell ... an amendment to the existing license between Histogen and ...
(Date:1/14/2014)... York, NY (PRWEB) January 14, 2014 ... of Alliqua, Inc. (OTCQB: ALQA). Alliqua is an emerging ... to serve the wound care market. , Free report ... was restructured with a seasoned management team and Board, ...
(Date:1/14/2014)... iLab Solutions, the leader in core facility management software, today ... In this role, Michelle will work with current and potential ... development of iLab products. Her main goal will be to ... scientific community by offering the most advanced, user-friendly, and high-impact ...
Breaking Biology Technology:TaiGen Biotechnology Signed Exclusive License Agreement with R-Pharm for Nemonoxacin (Taigexyn(R)) 2TaiGen Biotechnology Signed Exclusive License Agreement with R-Pharm for Nemonoxacin (Taigexyn(R)) 3TaiGen Biotechnology Signed Exclusive License Agreement with R-Pharm for Nemonoxacin (Taigexyn(R)) 4Histogen and Suneva Medical Expand License for Cell Conditioned Media-based Aesthetic Products Internationally 2Histogen and Suneva Medical Expand License for Cell Conditioned Media-based Aesthetic Products Internationally 3EquitiesIQ Initiates Coverage of Alliqua, Inc. 2EquitiesIQ Initiates Coverage of Alliqua, Inc. 3iLab Solutions Announces Michelle Detwiler as the New Director of Product Strategy 2
... the stress of the Second Lebanon War suffered fewer attacks ... focusing on feelings. This has been shown in a new ... the Technion-Israel Institute of Technology and Carmel Medical Center. "Because ... is important to uncover all the factors that impact the ...
... biggest Roman candle has got nothing on this. Using ... Earth or on a giant planet, Washington State University ... storing vast amounts of energy. "If you think about ... storage outside of nuclear energy," says Choong-Shik Yoo, a ...
... A major breakthrough in remote wave sensing by a ... for detecting hidden explosives, chemical, biological agents and illegal ... new, all-optical system, using terahertz (THz) wave technology, has ... it can "see through" clothing and packaging materials and ...
Cached Biology Technology:Can multiple sclerosis attacks be minimized in a war zone? 2A revolutionary breakthrough in terahertz remote sensing 2
(Date:4/18/2014)... Peru found that 28 percent of the batches ... Many pills released the active ingredient too slowly. ... had no active ingredient at all. , ... Georgia Institute of Technology developed a sophisticated approach ... drugs and then characterize their chemical composition. The ...
(Date:4/18/2014)... population predicted to reach 9 billion by 2050 ... considers human-altered landscapes such as farmland, according to ... that supports it might be an increasingly scarce ... of the land surface is directly affected by ... impacts such as climate change. But what if ...
(Date:4/18/2014)... Ancient DNA adds a twist to the story of ... be published April 21 in the journal Proceedings of ... the bones of chickens that lived 200-2300 years ago in ... domestic chickens may have looked far different from the chickens ... the traits we associate with modern domestic chickens -- such ...
Breaking Biology News(10 mins):Counterfeit contraceptives found in South America 2Counterfeit contraceptives found in South America 3Stanford researchers rethink 'natural' habitat for wildlife 2Stanford researchers rethink 'natural' habitat for wildlife 3Stanford researchers rethink 'natural' habitat for wildlife 4Ancient DNA offers clues to how barnyard chickens came to be 2
... , This release is available in ... thinks of herbal teas or valerian drops. However, that ... the Fraunhofer Institute for Molecular Biology and Applied Ecology ... to produce biopharmaceuticals. Those are proteins that, unlike many ...
... competitive realm of bacteria, cheaters must be kept in ... that in one bacterium, at least, bacterial cooperators can ... for dominance. "Even simple organisms such as ... Velicer, who with Ph.D. student Pauline Manhes has reported ...
... with rheumatoid arthritis are two times more likely to ... controls -- an association which was sustained even when ... controlled for, according to a study presented today at ... 15,766 patients with RA and 15,340 controls found that ...
Cached Biology News:Medicines from plants 2'Policing' stops cheaters from dominating groups of cooperative bacteria 2'Policing' stops cheaters from dominating groups of cooperative bacteria 3'Policing' stops cheaters from dominating groups of cooperative bacteria 4
Identify and characterize Phosphoinositide (PI(4,5)P2) binding...
... Moto Alpha unit with motorized heated lid and ... is useful for microarrays and chips. The four ... The sealing force of the motorized lid is ... give repeatable results. This interchangeable Moto Alpha unit ...
... Anti-Krueppel-like factor 15 (KLF15) KLF15 is ... found abundantly expressed in the liver, kidneys, ... NP_054798 Specificity: Polyclonal antibody produced in ... to a region of human KLF15 with ...
... For affinity chromatography, Affi-Prep protein ... (IgG), selectively remove IgG prior to ... adsorb immune complexes for antigen purification. ... region of immunoglobulins, especially IgG from ...
Biology Products: