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Heteroduplex Analysis Using the DCode System

rate the same as the wild-type. Under these conditions, it was not possible to resolve the two homoduplex bands of the ΔF508 mutant. This mutation may be detected if heteroduplexes are formed by mixing the mutant and wild-type samples together, then denaturing and renaturing the DNA.

The ability to resolve the heteroduplex bands in the mutant samples makes it possible to distinguish between the mutant and wild-type samples. Heteroduplex Analysis can be used as a method for screening heterozygous mutations in the cystic fibrosis gene using the DCode system.


References
1. Nagamine, C.M., Chan, K. and Lau, Y-F.C., Hum. Genet., 45, 337-339 (1989).

2. White, M., Carvalho, M., Derse, D., OBrien, S. and Dean, M., Genomics, 12, 301-306 (1992).


*The Polymerase Chain Reaction (PCR) process is covered by patents owned by Hoffmann-LaRoche. Use of the PCR process requires a license.


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