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HTRF IP-One Assay Performed on the PHERAstar and RUBYstar Plate Readers

/sup> cAMP assays. The new HTRF platform for GPCR screening will thus incorporate a series of HTS assays, all using similar protocols and a standard robotics and detectors. Such a platform obviously holds all that is needed to provide test solutions which are particularly well-adapted to meeting the challenges of the different stages of drug screening.


Conclusion
Cisbios new IP-One assay, which is based on its proprietary HTRF technology, is the first high throughput system that can easily detect inositol(1)phosphate (IP1), one of the major products of the phosphatidyl inositol cascade, which tightly correlates with Gq-coupled activity. In association with Cisbios cAMP assay, it allows the establishment of a complete HTRF based GPCRs platform fully covering the different signalling pathways triggerred by GPCRs. All HTRF IP-One assay results were produced on BMG LABTECHs dedicated HTRF plate reader RUBYstar and the multimode plate reader PHERAstar. The readers showed in a comparison strongly correlating results in terms of EC50 and Z' values.


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TAG: HTRF One Assay Performed the PHERAstar and RUBYstar Plate Readers

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