Navigation Links
HSVision Molecular Beacon Detection Module Rapidly Detects Herpes Simplex,,,Virus DNA

time PCR format, which provides rapid and sensitive detection with high-throughput capability, and significantly increase the speed, throughput, and reliability of HSV detection.

Molecular Beacon Technology

Molecular beacons are single-stranded oligonucleotides that possess a stem-and-loop hairpin structure.4-7 The loop is comprised of a DNA sequence complimentary to the target DNA, and the stem is formed by short complimentary sequences at opposite ends of the molecule. The ends of molecular beacons are labeled with a fluorophore and a quencher, respectively. When the molecular beacon is folded, the fluorophore and quencher are in close proximity, and fluorescence is quenched. However, when the molecular beacon is bound to a complimentary target, the fluorophore and quencher are separated, and the molecular beacon fluoresces (Figure 1).

Fig.1

Detecting Both HSV-1 and HSV-2 DNA

The HSVision module primers are derived from a 109 base-pair region of the gB gene that is highly conserved in HSV-1 and HSV-2 genomes.8 Because PCR reactions are often susceptible to a variety of inhibitors in biological specimens, an IAC is included in the HSV detection module to control for PCR failure. The IAC is a plasmid encoding a sequence identical in size and GC content to the HSV target DNA. The HSV-TC molecular beacon is labeled on the 5 end with the fluorophore tetrachloro 6-carboxyfluorescein (TET). The IAC-specific molecular beacon is labeled on the 5 end with 6-carboxyfluorescein (FAM). DABCYL is attached to the 3 end both molecular beacons and is used to quench fluorescence. The HSV-TC and IAC molecular beacons are multiplexed in a single-tube, real-time PCR reaction,
'"/>

Source:


Page: All 1 2 3 4

Related biology technology :

1. Detect Single Nucleotide Sequence Changes with Mx4000 Molecular Beacon Allelic Discrimination Kits
2. Genotyping Single Nucleotide Polymorphisms with Molecular Beacons
3. New Protocols for Isolating High- Molecular-Weight Genomic DNA
4. Molecular Biology:Easy Dephosphorylation and Rapid DNA Ligation
5. Isolation of Low Molecular Weight Digestion Products of the Human Platelet Thromboxane A2 Receptor, Rev A
6. Quantitation of GAD67 Gene Expression in Prefrontal Cortex of Schizophrenia Patients Using the iCycler iQ Detection System and Molecular Beacons, Rev A
7. Measurement of proteasome inhibition in live cells in Molecular Devices microplate fluorometers
8. Measurement of Molecular Beacons in the SpectraMax Gemini Spectrofluorometer (MaxLine Application Note #36)
9. Near Infrared Technology and Optical Agents For Molecular Imaging
10. Simple, Sensitive, and Rapid Detection of FLAG -Tagged Fusion Proteins
11. A New PCR-based Mycoplasma Detection Method
Post Your Comments:
(Date:8/21/2014)... for Molecular Medicine Finland (FIMM), University of Helsinki, ... "man and machine" decision support system for diagnosing ... in PLOS One scientific journal today, ... vision algorithms similar to those used in facial ... diagnostically most relevant areas. Tablet computers can be ...
(Date:8/21/2014)... 21, 2014 His Majesty Willem-Alexander, King ... The Court of His Majesty Willem-Alexander, King of the ... Grand Opening of POET-DSM Advanced Biofuels’ Project LIBERTY cellulosic ... , The King will take part in the Grand ... for 11 a.m.-12:20 p.m. and tour the plant. POET-DSM ...
(Date:8/21/2014)... Mathematic studies at the Program for Evolutionary ... by the Jeffrey Epstein VI Foundation , have shown ... identified for elimination. These aggressive cells are the driver mutations ... topological map of what to look for when examining tumors, ... tumor cells can be extracted from biopsy, it,s very difficult ...
(Date:8/21/2014)... August 21, 2014 Pursuit Solutions ... optimization technology and services for Life Sciences sales ... Pharma’s Use of New Commercial Sales Models” by ... Analytics. , The article examines some innovative models ... are being tested in the pharmaceutical market. Robinson ...
Breaking Biology Technology:A novel 'man and machine' decision support system makes malaria diagnostics more effective 2His Majesty Willem-Alexander, King of the Netherlands, to Attend Project LIBERTY opening 2His Majesty Willem-Alexander, King of the Netherlands, to Attend Project LIBERTY opening 3Science Power Jeffrey Epstein and Harvard University Fund Project to Visually Stop Tumor Growth 2Pursuit's Peter Robinson Publishes "Grading Pharma's Use of New Commercial Sales Models" 2
... Treatment Approved by the Fda For Hypotrichosis of,Eyelashes ... and Drug Administration (FDA) has approved LATISSE(TM),(bimatoprost ophthalmic ... the eyelashes. Eyelash hypotrichosis is another name for,having ... first and only,FDA-approved, science-based treatment to enhance eyelash ...
... WALTHAM, Mass., Dec. 26 Interleukin Genetics, Inc. (NYSE ... 2008 the Company received notice from the NYSE Alternext ... is not in compliance with certain conditions of the ... Company Guide. Specifically, the Exchange noted the Company,s failure ...
... Minimize Viral Transmission, Including Norovirus , A ... public health by minimizing the transmission of multiple ... providers. The researchers from the University of ... Raleigh report their findings in the August 2008 ...
Cached Biology Technology:MultiVu Video Feed: U.S Food and Drug Administration (Fda) Approves Latisse(TM) -- 2MultiVu Video Feed: U.S Food and Drug Administration (Fda) Approves Latisse(TM) -- 3Interleukin Genetics Receives Notification of Failure to Meet Continued Listing Standard Section 1003(a)(iii) 2Interleukin Genetics Receives Notification of Failure to Meet Continued Listing Standard Section 1003(a)(iii) 3Tips from the journals of the American Society for Microbiology 2Tips from the journals of the American Society for Microbiology 3Tips from the journals of the American Society for Microbiology 4
(Date:8/21/2014)... American singles found that during sex with a ... On average, men experience orgasm 85.1 percent of ... difference. For women, however, orgasm occurrence is less ... of the time during sex with a familiar ... sexual orientation, with lesbian women experiencing orgasm more ...
(Date:8/21/2014)... unknown process for harvesting energy and producing oxygen from ... University scientist has discovered. The discovery lays the foundation ... energy from the Sun, and understanding dense blooms like ... worldwide. A paper describing the discovery will be published ... journal Science on 21 August 2014. , ...
(Date:8/21/2014)... linked to dandruff, eczema and other itchy, flaky maladies ... global reachesincluding Hawaiian coral reefs and the extreme environments ... review in the scientific journal PLOS Pathogens ... of the genus Malassezia in light of ... around the world. , University of Hawai,i at Mānoa ...
Breaking Biology News(10 mins):Orgasm rates for single women less predictable than men's, vary by sexual orientation 2Orgasm rates for single women less predictable than men's, vary by sexual orientation 3Orgasm rates for single women less predictable than men's, vary by sexual orientation 4Hot-spring bacteria reveal ability to use far-red light for photosynthesis 2Hot-spring bacteria reveal ability to use far-red light for photosynthesis 3From dandruff to deep sea vents, an ecologically hyper-diverse fungus 2
... of Biological Sciences (AIBS) and the National Evolutionary ... and workshop for educators at the National Association ... The special symposium, which has become a ... classroom teachers with an opportunity to hear directly ...
... Research and Forestry Applications (CREAF), in the celebration of ... of international experts to discuss the environmental future of ... The work of both days is summarized in the ... Sustainability, which you will find bellow. Targeted to governments ...
... in age-related macular degeneration, the most common cause of ... today in the Lancet , adds to the ... (AMD), which the researchers believe should ultimately lead to ... people aged 80 years or older are affected by ...
Cached Biology News:Illuminating biology: An evolutionary perspective 2Illuminating biology: An evolutionary perspective 3Illuminating biology: An evolutionary perspective 4Barcelona Declaration 2008: Challenges and Pathways to Earth Sustainability 2Barcelona Declaration 2008: Challenges and Pathways to Earth Sustainability 3Barcelona Declaration 2008: Challenges and Pathways to Earth Sustainability 4Genetic finding implicates innate immune system in major cause of blindness 2
Request Info...
R26.4C...
... Specifications(L x W x H): 17 ... 5 5/16"18.7 lbsCapacity: 12 slidesTime: Programmable 0 ... Automated denaturation and hybridization steps 40 user ... only, and Fixed temperature) Rapid temperature ramp-up ...
Anti-DAP-1, Rabbit Monoclonal Immunogen: KLH-conjugated synthetic peptide corresponding to the C-terminal region of human DAP-1. Molecular Weight: 11 kDa Quality Assurance: Routinely eval...
Biology Products: