Navigation Links
HSVision Molecular Beacon Detection Module Rapidly Detects Herpes Simplex,,,Virus DNA

time PCR format, which provides rapid and sensitive detection with high-throughput capability, and significantly increase the speed, throughput, and reliability of HSV detection.

Molecular Beacon Technology

Molecular beacons are single-stranded oligonucleotides that possess a stem-and-loop hairpin structure.4-7 The loop is comprised of a DNA sequence complimentary to the target DNA, and the stem is formed by short complimentary sequences at opposite ends of the molecule. The ends of molecular beacons are labeled with a fluorophore and a quencher, respectively. When the molecular beacon is folded, the fluorophore and quencher are in close proximity, and fluorescence is quenched. However, when the molecular beacon is bound to a complimentary target, the fluorophore and quencher are separated, and the molecular beacon fluoresces (Figure 1).

Fig.1

Detecting Both HSV-1 and HSV-2 DNA

The HSVision module primers are derived from a 109 base-pair region of the gB gene that is highly conserved in HSV-1 and HSV-2 genomes.8 Because PCR reactions are often susceptible to a variety of inhibitors in biological specimens, an IAC is included in the HSV detection module to control for PCR failure. The IAC is a plasmid encoding a sequence identical in size and GC content to the HSV target DNA. The HSV-TC molecular beacon is labeled on the 5 end with the fluorophore tetrachloro 6-carboxyfluorescein (TET). The IAC-specific molecular beacon is labeled on the 5 end with 6-carboxyfluorescein (FAM). DABCYL is attached to the 3 end both molecular beacons and is used to quench fluorescence. The HSV-TC and IAC molecular beacons are multiplexed in a single-tube, real-time PCR reaction,
'"/>

Source:


Page: All 1 2 3 4

Related biology technology :

1. Detect Single Nucleotide Sequence Changes with Mx4000 Molecular Beacon Allelic Discrimination Kits
2. Genotyping Single Nucleotide Polymorphisms with Molecular Beacons
3. New Protocols for Isolating High- Molecular-Weight Genomic DNA
4. Molecular Biology:Easy Dephosphorylation and Rapid DNA Ligation
5. Isolation of Low Molecular Weight Digestion Products of the Human Platelet Thromboxane A2 Receptor, Rev A
6. Quantitation of GAD67 Gene Expression in Prefrontal Cortex of Schizophrenia Patients Using the iCycler iQ Detection System and Molecular Beacons, Rev A
7. Measurement of proteasome inhibition in live cells in Molecular Devices microplate fluorometers
8. Measurement of Molecular Beacons in the SpectraMax Gemini Spectrofluorometer (MaxLine Application Note #36)
9. Near Infrared Technology and Optical Agents For Molecular Imaging
10. Simple, Sensitive, and Rapid Detection of FLAG -Tagged Fusion Proteins
11. A New PCR-based Mycoplasma Detection Method
Post Your Comments:
(Date:5/26/2015)... Germany , May 26, 2015 ... QGEN ; Frankfurt Prime Standard: QIA) today announced ... ® (QCI™) bioinformatics content and software platform ... on genomic variants identified in next-generation sequencing (NGS). ... platform are in oncology, for somatic and hereditary ...
(Date:5/26/2015)... FL (PRWEB) May 26, 2015 ... Inc. have announced plans for the company’s first cellular ... 2015, followed by a full day, hands-on stem cell ... by J. Victor Garcia Gimenez, M.D., President of Therapeutic ... the medical community’s increasing interest in regenerative medicine and ...
(Date:5/26/2015)... San Diego, CA (PRWEB) May 26, 2015 ... Biopharma, announced the beginning of a long-term relationship ... works on behalf of its members to provide ... “I am extremely pleased to count VGP as ... to more arthritic dog patients nationwide. Concentrating and ...
(Date:5/26/2015)... TORONTO , May 26, 2015 /PRNewswire/ - Fluorinov ... appointment of Luke M. Beshar to its ... adding financial and pharmaceutical industry expertise as Fluorinov accelerates ... of the Fluorinov team, it is a pleasure to ... had a significant impact on the strategy, financial strength ...
Breaking Biology Technology:QIAGEN Launches Clinical Bioinformatics Platform for Next-Generation Sequencing 2QIAGEN Launches Clinical Bioinformatics Platform for Next-Generation Sequencing 3QIAGEN Launches Clinical Bioinformatics Platform for Next-Generation Sequencing 4QIAGEN Launches Clinical Bioinformatics Platform for Next-Generation Sequencing 5Stem Cell Training, Inc. Announces First European Symposium on Cellular and Regenerative Medicine 2Stem Cell Training, Inc. Announces First European Symposium on Cellular and Regenerative Medicine 3VetStem Biopharma & Veterinary Growth Partners Join Forces to Increase Member Veterinary Hospital Revenue Through Cutting Edge Medical Offering 2VetStem Biopharma & Veterinary Growth Partners Join Forces to Increase Member Veterinary Hospital Revenue Through Cutting Edge Medical Offering 3Luke M. Beshar Appointed to Fluorinov Pharma Board of Directors 2Luke M. Beshar Appointed to Fluorinov Pharma Board of Directors 3
... Aug. 10, 2011 Genesis Biopharma, Inc. (OTC/BB: ... today announced it has signed a Cooperative Research ... Institute (NCI).  Under the terms of the five-year ... work with Steven A. Rosenberg, M.D., Ph.D., the ...
... Accuray Incorporated (Nasdaq: ARAY ), the premier ... to present at the Wedbush Life Sciences: Management Access Conference ... a.m. ET/6:10 a.m. PT. A live webcast of ... page of the Company,s Web site at http://www.accuray.com . ...
... YORK, Aug. 9, 2011 Reportlinker.com ... report is available in its catalogue: ... Market: Facilities, Test Volumes, and Sales ... http://www.reportlinker.com/p0591318/Global-Microbiology-Testing-Market-Facilities-Test-Volumes-and-Sales-Forecasts-by-Country.html#utm_source=prnewswire&utm_medium=pr&utm_campaign=In_Vitro_Diagnostic This ...
Cached Biology Technology:Genesis Biopharma Signs Cooperative Research and Development Agreement With the National Cancer Institute to Develop Cancer Immunotherapies 2Genesis Biopharma Signs Cooperative Research and Development Agreement With the National Cancer Institute to Develop Cancer Immunotherapies 3Global Microbiology Testing Market: Facilities, Test Volumes, and Sales Forecasts by Country 2
(Date:5/19/2015)... YORK , May 19, 2015  Technology is ... both internally and in the cloud. Passwords and their ... use of OTP and standards-based specifications such as those ... the outmoded use of passwords presents for BYOD, COPE, ... a unified biometric identity protocol. In ...
(Date:5/14/2015)... --Research and Markets ( http://www.researchandmarkets.com/research/5whpxg/fingerprint ) has ... in Smart Mobile Devices " report to their ... a watershed year for fingerprint sensors in smartphones and ... gave fingerprint sensors a raison d,etre in the mobile ... a must-have feature in flagship smartphones. The ...
(Date:5/11/2015)... DAYTON, Ohio , May 11, 2015  Through a ... Ohio had a strong showing at AUVSI,s ... . These representatives of Ohio,s UAS ... domestic and abroad from all points along the UAS ecosystem. ... Dayton Development Coalition,s (DDC) Vice President for Aerospace Rich ...
Breaking Biology News(10 mins):HYPR Corp. Biometric Security Integration Kit Shields Applications and Devices Across the Internet of Things 2HYPR Corp. Biometric Security Integration Kit Shields Applications and Devices Across the Internet of Things 3Global Smart Mobile Devices Fingerprint Sensors Market 2015-2019: Key Vendors are Authentec, Validity Sensors, Fingerprint Cards, Next Biometrics Group. IDEX, and Qualcomm 2Ohio Flies High at Unmanned '15, Sets Stage for Ohio UAS Conference 2
... University of Jerusalem researchers of an additional role for a ... world-wide efforts to develop genetic regulation aimed at controlling many ... The molecule, known as Lysyl-tRNA synthetase (or LysRS in brief) ... where it has long been recognized for its contribution in ...
... to be able to ward off disease pathogens, immune cells ... each other. The working group around Professor Dr. Oliver Fackler ... Heidelberg University Hospital has discovered a mechanism in an animal ... Cell mobility is inhibited by the HIV Nef protein. The ...
... its workshop on freezing of human sperm, eggs and embryos ... embryos". The workshop provides a forum for clinicians and scientists ... Here a few of the new research findings ... announce: Vitrification of reproductive cells: the ...
Cached Biology News:How HIV cripples immune cells 2
...
...
... silica based spin filter membrane to isolate DNA ... band is cut from an agarose gel and ... gel slice is melted irreversibly in a high ... to the silica spin filter membrane and washed ...
...
Biology Products: