Single-tube amplification method for high-throughput screening of HSV
Cindy Walker-Peach Beti Belachew Peter Pingerelli
Melanie Palmer Dwight DuBois
Stratagene introduces the HSVision molecular beacon detection module,*,,ff a single-tube method to quickly detect HSV (herpes simplex virus) DNA. Because it is performed in a closed-tube system and requires no postamplification manipulation, HSV detection is simplified, and the risk of contamination is markedly reduced. Using molecular beacon technology, this detection module includes an HSV molecular beacon that hybridizes to both HSV-1 and HSV-2 amplicons, a molecular beacon specific for an internal amplification control (IAC), a mixed HSV-1 and HSV-2 DNA standard, target-specific DNA primers, and an optimized PCR buffer. This module can be easily adapted for high-throughput screening
HSVs have worldwide distribution and cause a wide variety of illnesses in humans. Since this virus remains latent in the host for life, it can be reactivated to cause lesions at or near the site of initial infection.1 Most common are mucocutaneous infections, which primarily affect oral-facial and genital surfaces. However, more serious HSV infections can also involve the central nervous system and viscera, particularly in newborns and immunocompromised patients, where they are often life threatening. There are two HSV subtypes, HSV-1 and HSV-2,2 which are serologically and genetically distinct. HSV-1 and HSV-2 share a similar overall genomic structure and contain approximately 50% nucleotide sequence identity.
Current diagnostic methods for HSV infection include serological testing,
viral culture, and more recently, PCR detection.3 Stratagene has
developed a single-tube, real-