Navigation Links
HL-60

Multiporator Transfection Protocol Protocol No. 4308 915.028 11/1999 Cell line HL-60, human monocyte like cells (suspension cell line) Transfection with Plasmid pEGFP-N1 (in bidistilled H2O) Electroporation buffer Eppendorf Hypoosmolar Electroporation Buffer (PH) Culture medium RPMI 1640 / 10% FCS Cuvette Eppendorf, 4 mm gap width, 800 l Temperature RT (20-25 C) Reference Dr. Hans Hcker Institut fr Med. Mikrobiologie, Immunologie und Hygiene
Technische Universitt Mnchen Trogerstr. 4a D-81675 Mnchen
Phone +49 89 4140 4184 Fax +49 89 4140 4183 e-mail: hans.haecker@lrz.tu-muenchen.de
  1. Harvest the cells in the exponential growth phase and centrifuge them (for 5-10 minutes, 200 x g, at room temperature).
  2. Resuspend the cells in RPMI 1640 / 0.5% FCS, determine the number of cells and centrifuge them (for 5-10 minutes, 200 x g, at room temperature). Remove supernatant.

    Note: The overall incubation time in the Eppendorf Electroporation Buffer must not exceed 30 minutes to guarantee a successful electroporation!

  3. Resuspend the cells in Hypoosmolar Electro
    '"/>


Source:


Page: All 1 2

Related biology technology :

1. Primary cells, rat heart endothel
2. Primary cells, rat heart muscle
Post Your Comments:
*Name:
*Comment:
*Email:
TAG:

(Date:1/22/2015)... Protocol Networks brings independent technology consulting to the ... consultants, Protocol Networks has recently announced its expansion into ... his company has attracted several clients in The Constitution ... as others. With the success of these engagements, Rogers ...
(Date:1/22/2015)... 22, 2015 Dr. Greg Leyer of UAS ... Scripps Natural Supplements Pre-Conference seminar on probiotics in San Diego, ... annual continuing education conference for health care professionals. This year’s ... topic of probiotics in health. Dr. Leyer spoke about the ...
(Date:1/22/2015)... , Nueva Jersey , 22 de enero de ... Biomedical Research abre hoy su llamada a nominaciones para ... investigación científica ha hecho, o tiene el potencial para ... humana. Las nominaciones se aceptarán hasta el 15 de ...
(Date:1/22/2015)... N.J. , Jan. 22, 2015  Derma Sciences, ... regeneration company focused on advanced wound care, announces ... placental tissues, have been added to the Premier, ... increases the opportunity for the AMNIOEXCEL® and AMNIOMATRIX® ...
Breaking Biology Technology:Protocol Networks Brings Independent Technology Consulting to the Constitution State 2El Dr. Paul Janssen Award for Biomedical Research emite su llamada a los nominados para 2015 2El Dr. Paul Janssen Award for Biomedical Research emite su llamada a los nominados para 2015 3El Dr. Paul Janssen Award for Biomedical Research emite su llamada a los nominados para 2015 4Derma Sciences Expands Access of its AMNIOTIC TISSUE Product Line with New Premier, Inc. Agreement 2Derma Sciences Expands Access of its AMNIOTIC TISSUE Product Line with New Premier, Inc. Agreement 3Derma Sciences Expands Access of its AMNIOTIC TISSUE Product Line with New Premier, Inc. Agreement 4Derma Sciences Expands Access of its AMNIOTIC TISSUE Product Line with New Premier, Inc. Agreement 5
... DIEGO, Jan. 12, 2011 Sequenom (Nasdaq: ... in the British Medical Journal (BMJ 2011;342:c7401) of a ... Dennis Lo of The Chinese University of Hong Kong. ... present paper is the first report of a large-scale ...
... The VWR Charitable Foundation, in partnership with VWR ... distribution company, hosted an auction last night at the ... to benefit the Orlando Science Center. "Everyday, ... scientific explorers, and creates a hands-on experience that fosters ...
... Regenicin, Inc. (OTC Bulletin Board: RGIN ) ... cell therapies to restore the health of damaged tissues ... at the Sir Francis Drake Hotel in San Francisco ... present Regenicin,s core business model and strategic approach for ...
Cached Biology Technology:Professor Dennis Lo Trisomy 21 Clinical Validation Study Published in British Medical Journal 2VWR Charitable Foundation to Support the Orlando Science Center with Auction 2VWR Charitable Foundation to Support the Orlando Science Center with Auction 3Regenicin CEO to Participate in Live Webcast at OneMedForum Business Development and Finance Conference, January 11th at 4:00 PM PST 2
(Date:1/22/2015)... 2015   EyeLock, Inc. , a market leader of iris-based ... Gerber to the new role of Senior Vice President ... development of mobile platforms and wearable solutions for EyeLock,s technologies. ... in the semiconductor industry to his role at EyeLock, with ...
(Date:12/22/2014)... DENVER , Dec. 22, 2014  The 2014 Holiday ... industry as Acuity Market Intelligence reports that the long ... Acuity forecasts that intensifying demand for smart phones, tablets, ... a global market of 2.5 billion users with nearly ...
(Date:12/17/2014)... , Dec. 15, 2014 Research and Markets ... the "Samsung Galaxy S5 - Home Button Synaptics ... http://photos.prnewswire.com/prnh/20130307/600769 ... iPhone 5S, Samsung introduces for the first time a ...
Breaking Biology News(10 mins):EyeLock Names Steve Gerber as Senior Vice President of Mobile and Wearables 2First Season of Holiday Shopping with Mobile Biometric Payments Wraps Up With a Present for Biometrics Industry: A Rosy Forecast for More Than 2 Billion Users of 4.8 Billion Mobile Biometric Devices by 2020 2Samsung Galaxy S5 - Home Button Synaptics Fingerprint Sensor Technology Analysis 2
... (UC) researchers have received a grant in excess of $2 ... to determine the ideal time to fly that minimizes health ... them from the battlefield into a safe zone. ... soldiers to a cleaner, safer environment," explains Col. Warren Dorlac, ...
... . Within the last few years the number ... been increased considerably. A worthwhile effort as, after all, it ... methods of transport checks are offered by terahertz radiation. Before ... has to be measured quantitatively, so that damage to health ...
... producing significant amounts of a chemical form of aspirin, ... National Center for Atmospheric Research (NCAR), opens up new ... their impacts on air quality, and it also has ... about crops that are failing. "Unlike humans, who ...
Cached Biology News:US Air Force grant targets medical evacuation procedures 2US Air Force grant targets medical evacuation procedures 3US Air Force grant targets medical evacuation procedures 4Checking people at airports -- with terahertz radiation 2Plants in forest emit aspirin chemical to deal with stress; discovery may help agriculture 2Plants in forest emit aspirin chemical to deal with stress; discovery may help agriculture 3
Anti-MMP-25 (Membrane-type MMP-6, Mt6MMP), hinge region; rabbit host...
Anti-MMP-25 (Membrane-type MMP-6, Mt6MMP), hinge region; rabbit host...
Desmosomal Protein Immunogen: Full length, native protein purified from human epidermal keratins. Storage: -20 C, Avoid Freeze/Thaw Cycles...
PPIF Antibody...
Biology Products: