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Figure 1. MessageSensor RNase H⁺ RT vs. an RNase H RT Containing Kit. Primers to the Na⁺/Ca²⁺ exchanger gene NCX2 were used to perform RT-PCR on 100 ng of Rat Brain Total RNA (Ambion). The amplicon is 452 bases. After 24 cycles, samples were loaded on a 1.5% agarose gel and stained with EtBr. Duplicate reactions were performed for each RT-PCR kit. RT minus controls were negative for both kits.

Figure 2. One Step RT-PCR With MessageSensor System and a Popular MMLV RT RNase H System. C_t values associated with cdc-2 and -actin targets were detected from 10 pg HeLa-S3 total RNA, whereas c-jun was detected from 100 ng HeLa-S3 total RNA. Note: A lower C_t value indicates a more sensitive detection system. 40 cycles represents no product detected (ND).

MessageSensor: RNA to PCR Results in 2 hrs
MessageSensor provides unsurpassed results in the least amount of time. The kit contains a unique formulation of MMLV RT developed for a one tube real-time RT-PCR approach to create a highly optimized protocol and reagent system. The result is a kit that delivers rapid results without compromising lower-end sensitivity. Simply prepare a master mix that combines your RNA sample, primers, and dye-based reporter. Add Taq DNA polymerase, with the MessageSensor RNase H⁺ reverse transcriptase, buffer
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