Navigation Links
H73C11

fer (= 68% Eppendorf Hypoosmolar Electroporation Buffer + 32% Eppendorf Isoosmolar Electroporation buffer). When doing so, set the cell concentration to 1 x 106 cells/ml.
  • Add and mix plasmid DNA (10-20 g/ml final concentration, in bidistilled H2O).
  • Transfer 400 l cell suspension into electroporation cuvettes (2 mm gap width). The cell suspension must be free of air bubbles.
  • Electroporation:

    Mode Eukaryotes Voltage (V) 160 V Time constant (T) 40 s No. of pulses (n) 1
  • After the pulse, allow the cell suspension to stand in the cuvette for 5-10 minutes at room temperature.
  • Carefully transfer the cell suspension from the cuvette to 5 ml RPMI 1640 / 10% FCS, and cultivate it in a 60 mm culture dish.
    Note: After pulsing, the cells should be incubated for 2-3 h at 37 C before any centrifugation is performed, to ensure resealing of the membrane.
  • Detection methods for transfection:
    The expression of the plasmid pEGFP-N1 can be detected clearly after 24-48 hours with the aid of FACS analysis or under a fluorescence microscope. Result: Survival rate: 250% Transfection rate: 20% based on the initial number of cells used for the experiment
    '"/>

    Source:


    Page: All 1 2 3

    Related biology technology :

    1. Primary cells, rat heart endothel
    2. Primary cells, rat heart muscle
    Post Your Comments:
    *Name:
    *Comment:
    *Email:
    TAG:

    (Date:9/19/2014)... , Sept. 19, 2014 Pfenex Inc. ... the development of high-value and difficult to manufacture proteins ... presenting at the 21 st Annual NewsMakers in ... . Bertrand Liang , chief executive officer ... development programs and business strategy on Friday, September 26, ...
    (Date:9/19/2014)... September 19, 2014 Barnhardt Manufacturing ... Label for its Organic Cotton . The USDA ... of renewable biobased ingredients meets or exceeds levels set ... composed in whole or in significant part of agricultural, ... allow our Organic cotton to be immediately identified as ...
    (Date:9/19/2014)... , September 19, 2014 ... Markets ( http://www.researchandmarkets.com/research/b2jxhs/micro_market ) has announced the addition of ... Columns"  report to their offering.       ... is one of the fastest-growing segments in the ... billion in 2013, and expected to reach $2.0 ...
    (Date:9/19/2014)... September 19, 2014 The new , ... , available today on PharmaBoardroom.com for ... crisis that rocked the Spanish economy in 2009, and the ... community has remained pragmatic in times of trouble. ... the fifth biggest European market, and the general conditions to ...
    Breaking Biology Technology:Pfenex to Present at Upcoming Industry Conference on September 26 2Barnhardt Manufacturing Company Earns USDA Certified Biobased Product Certification and Label for Organic Cotton 2Micro Market Monitor : Global Pre-packed Chromatography Columns 2PharmaBoardroom Releases New Spain Pharmaceuticals Report 2PharmaBoardroom Releases New Spain Pharmaceuticals Report 3
    ... (Nasdaq: SGMO ) today reported second quarter 2011 ... quarter ended June 30, 2011, Sangamo reported a consolidated net ... a net loss of $3.9  million, or $0.09 per share, ... 2011, the company had cash, cash equivalents and marketable securities ...
    ... 2011 Once routinely discarded during the birth process, ... life-saving stem cells that can be collected and stored ... and vast medical potential, published data shows that three ... about cord blood banking. In connection with ...
    ... Calif., July 26, 2011 Synterra Energy, Inc. ... and assets for waste biomass conversion from Pacific ... Lion Bio-Energy (Toledo, Ohio).  With the merger, Synterra ... bio-refineries for the economic production of renewable fuels, ...
    Cached Biology Technology:Sangamo BioSciences Reports Second Quarter 2011 Financial Results 2Sangamo BioSciences Reports Second Quarter 2011 Financial Results 3Sangamo BioSciences Reports Second Quarter 2011 Financial Results 4Sangamo BioSciences Reports Second Quarter 2011 Financial Results 5Sangamo BioSciences Reports Second Quarter 2011 Financial Results 6Sangamo BioSciences Reports Second Quarter 2011 Financial Results 7Parent's Guide to Cord Blood Foundation Launches Nation's First Searchable Map of U.S. Sites for Donating Umbilical Cord Blood 2Parent's Guide to Cord Blood Foundation Launches Nation's First Searchable Map of U.S. Sites for Donating Umbilical Cord Blood 3Synterra Energy Merges Key Technologies to Commercialize Next Generation Integrated Bio-refinery 2
    (Date:9/18/2014)... Sept. 18, 2014 -- Arizona turned its newborn screening program ... few short months, earning it the first-ever Newborn Screening Quality ... of Dimes presented Will Humble, M.P.H., Arizona,s Department of Health ... today. Mr. Humble established a policy of full transparency for ... newborn blood samples to the lab for analysis, and set ...
    (Date:9/18/2014)... of carbon and nitrogen comprise all spheres of ... systems have caused imbalances and perturbations that resulted ... Increased GHGs result in climate changes which influence ... and the atmosphere. Understanding these feedback mechanisms has ... environmental science during the past three decades. , ...
    (Date:9/18/2014)... can swim up to 400 kilometres in search of a ... change. , Clownfish spend their entire adult lives under the ... wander the open ocean, says study co-author, Dr Hugo Harrison ... (Coral CoE) at James Cook University. , "In the past ... given a rare glimpse into how far they can swim, ...
    Breaking Biology News(10 mins):March of Dimes honors Arizona with first-ever newborn screening award 2March of Dimes honors Arizona with first-ever newborn screening award 3Greenhouse gas research enters a new era 2Nemo's epic journey to find a new home 2
    ... A team led by researchers at the University ... the "superbug" methicillin-resistant Staphylococcus aureus (MRSA) is ... is well known for causing difficult-to-treat and potentially fatal ... 1990s it has also been infecting otherwise healthy people ...
    ... study shows for the first time that episodes of ... the 2001-02 drought, greatly accelerated development of the mountain ... to chart the evolution of the current pine beetle ... beetle outbreak in the two primary host species, the ...
    ... a daily multivitamin, but little is known about a multivitamin,s ... Brigham and Women,s Hospital (BWH) finds that daily multivitamin use ... A similar BWH study, announced last month, found daily ... 8 percent. The cardiovascular disease findings will be presented ...
    Cached Biology News:Superbug MRSA identified in US wastewater treatment plants 22001-2002 drought helped propel mountain pine beetle epidemic, says CU study 22001-2002 drought helped propel mountain pine beetle epidemic, says CU study 32001-2002 drought helped propel mountain pine beetle epidemic, says CU study 4Daily multivitamin use does not reduce cardiovascular disease risk in men 2
    ... SNPstream System Delivers 4,600 to Over 3 ... Genotyping System is capable of performing 4,600 ... Part of the GenomeLab family of products*, ... up to 48 SNPs in each well ...
    Mouse monoclonal antibody to ACP2 - acid phosphatase 2, lysosomal...
    Mouse monoclonal antibody to DCAMKL2 - doublecortin and CaM kinase-like 2...
    ... Tube Disperser System from IKA Works is the ... cleaner, safer, and more efficient way to disperse ... 2 to 15 ml inside sealed tubes. Depending ... of 4 different tube types. Each tube is ...
    Biology Products: