Navigation Links
H73C11

Multiporator Transfection Protocol Protocol No. 4308 915.024 11/2001 Cell line H73C11, mouse-human, heteromyeloma (suspension cell line) Transfection with Plasmid pEGFP-N1 (in bidistilled H2O) Electroporation buffer Eppendorf Electroporation Buffer with 150 mOsmol/kg Culture medium RPMI 1640 / 10% FCS Cuvette Eppendorf, 2 mm gap width, 400 Temperature RT (20-25 C) Reference Prof. Ulrich Zimmermann Lehrstuhl fr Biotechnologie Biozentrum Universitt Wrzburg
Am Hubland D-97074 Wrzburg Phone +49 931 888 4508 Fax +49 931 888 4509
e-mail: zimmerma@biozentrum.uni-wuerzburg.de
  1. Harvest the cells in the exponential growth phase and centrifuge them (for 5-10 minutes, 200 x g, at room temperature).
  2. Resuspend the cells in RPMI 1640 / 0.5% FCS, determine the number of cells and centrifuge them (for 5-10 minutes, 200 x g, at room temperature). Remove supernatant.

    Note: The overall incubation time in the Eppendorf Electroporation Buffer must not exceed 30 minutes to guarantee a successful electroporation!

  3. Resuspend the cells in 150 mOsmol/kg Electroporation Buf
    '"/>


Source:


Page: All 1 2 3

Related biology technology :

1. Primary cells, rat heart endothel
2. Primary cells, rat heart muscle
Post Your Comments:
*Name:
*Comment:
*Email:
TAG:

(Date:12/19/2013)... , Dec. 19, 2013  NeuroPhage Pharmaceuticals, ... an $822,000 grant by The Michael J. Fox ... drug candidate, NPT088, based on NeuroPhage,s GAIM (general ... misfolded proteins central to many neurodegenerative diseases. In ... research the effect of NPT088 on alpha-synuclein deposits ...
(Date:12/19/2013)... December 19, 2013 Top high school ... at the 55th Annual FiberMax® Caprock Classic in Lubbock, ... consecutive year that FiberMax cotton from Bayer CropScience has ... premier high school basketball tournaments. , The tournament is ... Clubs (AMBUCS), a nonprofit organization dedicated to creating independence ...
(Date:12/19/2013)... Mass. , Dec. 19, 2013  Decision Resources, ... for pharmaceutical and healthcare issues, finds that novel biologics ... with currently listed agents for a given disease to ... Argentina , Brazil ... the new Strategic Insights report entitled Trends ...
(Date:12/19/2013)... Finding a dead mouse in the lab ... lab is a Biosafety Level-3 containment facility designed to keep ... brain tumor that shines green under blue light, it’s time ... it’s time to panic. Especially when they could be spreading ... " Not Easy Being Green " is the second in ...
Breaking Biology Technology:NeuroPhage Awarded Second Grant from Michael J. Fox Foundation for Parkinson's Disease Research 2NeuroPhage Awarded Second Grant from Michael J. Fox Foundation for Parkinson's Disease Research 3NeuroPhage Awarded Second Grant from Michael J. Fox Foundation for Parkinson's Disease Research 455th Annual FiberMax Caprock Classic Basketball Tourney Is Largest in Nation 255th Annual FiberMax Caprock Classic Basketball Tourney Is Largest in Nation 355th Annual FiberMax Caprock Classic Basketball Tourney Is Largest in Nation 4New Biologics Must Show Improvements in Efficacy, Safety and Cost-Benefit Compared with Currently Available Agents for Inclusion on Formularies in Argentina, Brazil and Mexico 2A New Kind of Medical Thriller in Susy Gage's "Not Easy Being Green," Coming 2015 from Bitingduck Press 2
... RNAi analysis affordable and accessible to all researchers, ... Inc.,announced today that Harvard Medical School and the ... RNAi Program. Each University,purchased access to whole genome ... RNAi Consortium and distributed by Open Biosystems., ...
... Limited (TSX: GEN),a company focused on developing molecular ... the early detection of diseases and personalized,health management, ... the design and development of in-vitro diagnostic (IVD) ... status in a laboratory,setting, has met the requirements ...
... Sweden, March 11 Mentice AB, a leading,supplier of ... has recently appointed James Ruscoe as Chairman of the ... Mr. Ruscoe graduated from Oxford University and brings ... He also serves as CEO and,board member of several ...
Cached Biology Technology:Open Biosystems' Open Access RNAi Program Adopted by Harvard Medical School and University of North Carolina 2GeneNews receives ISO 13485 accreditation 2Mentice AB Announces Leadership Changes Within Its Worldwide Operations 2
(Date:12/19/2013)... dioxide is poised to join the pantheon of superstars ... ability to change size, shape and physical identity, vanadium ... A team of researchers with the U.S. Department of ... demonstrated a micro-sized robotic torsional muscle/motor made from vanadium ... more powerful than a human muscle, able to catapult ...
(Date:12/19/2013)... New York have reported the successful transformation of ... sugars as a structuring agent. The findings portend ... produced using saturated/trans fatty acids, which have been ... , The team, led by City College Professor ... gelators, mannitol dioctanoate (M8) and sorbitol dioctanoate (S8), ...
(Date:12/19/2013)... 19, 2013  The real world is about to ... the most accurate facial recognition software in the world, ... send it wirelessly to a server, compare it to ... complete with a name, additional photos and social media ... also currently creating technology to allow the scanning of ...
Breaking Biology News(10 mins):A micro-muscular breakthrough 2A micro-muscular breakthrough 3CCNY chemists use sugar-based gelators to solidify vegetable oils 2FacialNetwork.com Announces Beta Release Of "NameTag" The First Real-Time Facial Recognition App For Google Glass 2
... National Security Agency began eavesdropping on the phone calls ... learning the tunes sung by their neighbors. , University ... melodia) acquire their song repertoires have found the first ... by eavesdropping on the tuneful interactions between other sparrows. ...
... New research conducted at the Uniformed Services University of ... possible to develop a vaccine that protects against the ... variable, so an effective vaccine may need to stimulate ... multiple viral strains. These results demonstrate that induction of ...
... die at the first signs of cold weather. Rather, new ... to survive the chilly months. , These so-called "heat-shock proteins" ... come spring. , A study of flesh flies and ... arsenal of protective heat-shock proteins that are turned on almost ...
Cached Biology News:Eavesdropping comes naturally to young song sparrows 2Eavesdropping comes naturally to young song sparrows 3For many insects, winter survival is in the genes 2For many insects, winter survival is in the genes 3
Kit includes your choice of reagents for labeling 3 x 10 9 cells and EasySep magnet....
... RoboSep is the first instrument to offer ... column-free EasySep technology to isolate cells by either ... separate up to 4 samples at one time. ... screen. The intuitive graphical user interface makes it ...
... 1.5mg of purified RNA samples., , This ... of aqueous RNA samples that have been ... phenol/chloroform, silica, oligo dT etc or other ... the necessary components for the stabilization of ...
... 50g of purified RNA samples for RT-PCR and ... of the advantages and applications you expect from ... can be used for enzymatic applications such as ... optimised for the stabilization of aqueous RNA samples ...
Biology Products: