Navigation Links
Greater Amplification Specificity with New Hot Start PCR Enzyme

mplify the 115-bp gag gene fragment (Figure 2) were 1 cycle at 95C for 1 minute (Taq and Taq plus hot start antibody) or 10 minutes (SureStart Taq), followed by 40 cycles at 95C for 1 minute (denaturation); 55C for 1 minute (annealing); and 72C for 1 minute, followed by one final extension cycle of 72C for 10 minutes. The conditions used to amplify the 970-bp lambda DNA fragment (Figure 3) were 1 cycle at 93C for 1 minute (Taq and Taq plus hot start antibody), 10 minutes (SureStart Taq) or 10 to 15 minutes (competitors modified Taq preparations); 45 cycles at 93C for 1 minute (denaturation); 60C for 50 seconds (annealing); and 68C for 1 minute, followed by one final extension cycle of 68C for 10 minutes.


REFERENCES

1. Nielson, K.B., Cline, J., and Hogrefe, H. (1997) Strategies 10: 40-43.
2. Nielson, K., et al. (1991) Strategies 4: 38.
3. Bai, F. and Hogrefe, H. (1999) Strategies 12: 14-16.
4. Mullis, K. (1991) PCR Methods Appl. 1: 1-4.
5. Aquila, R.T., et al. (1991) Nucl. Acids Res. 19: 3749.
6. Chou, Q., et al. (1991) Nucl. Acids Res. 20: 7.
7. Erlich, H.A., Gelfand, D., and Sninsky (1991) Science 252: 1643-1651.
8. Sharkey, D.J., et al. (1994) BioTechnology 12: 506-509.
9. Lin, Y. and Jayasena, S.D. (1997) J. Mol. Biol. 271: 100-111.
10. Birch, D.E., et al. (1996) Nature 381: 445-446.
11. Nielson, K.B., et al. (1996) Strategies 9: 7-8.
12. Padmabandu, G. et al (2000) Strategies 13: 88-92.


'"/>

Source:


Page: All 1 2 3 4 5 6 7

Related biology technology :

1. Enjoy a Greater Sense of Security with PCR-Grade Water
2. prostar RT-PCR Systems for Robust High-Fidelity RNA Amplification
3. Simple Purification of DNA from Plasmid Minipreps, PCR Amplifications and Agarose Gels
4. Enhanced Amplification of Long Targets with PfuTurbo DNA Polymerase
5. Optimizing pfuturbo DNA Polymerase Amplification Reactions with Perfect Match PCR Enhancer
6. Improve Amplification Specificity with Hot Start PCR Enzyme
7. Amplification of the amoA Gene of Nitrosococcus oceani Bacteria Using Eppendorf MasterTaq
8. Amplification of Mouse cDNAs for Microarrays Using the Eppendorf MasterTaq Kit
9. Optimizing the Direct Amplification of Missing cDNA 5 Ends Using the Eppendorf Mastercycler gradient
10. Optimizing DNA Amplification Protocols using the Eppendorf Mastercycler
11. Amplification of Epstein-Barr Virus Exon C with Difficult Primers Using Eppendorf Mastercycler gradient
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Greater Amplification Specificity with New Hot Start PCR Enzyme

(Date:12/19/2014)... LayerBio is an MIT spin-off developing novel drug ... Eye Institute (NEI) at the National Institutes of Health ... to develop a drug-eluting intraocular lens (IOL) for cataract ... vision loss in people over age 40 and the ... Mandell, LayerBio’s Founder and CEO, "There is a critical ...
(Date:12/19/2014)... In contrast to traditional LED lights where the spectrum ... proprietary LED technology to incorporate their spectra in a ... critical in low proximity installations. The Valoya L-series offers ... which is up to four times longer than commonly ... was clearly higher when compared to traditional fluorescent or ...
(Date:12/19/2014)... MILLBROOK, N.Y. , Dec. 18, 2014 Egenix, ... has been elected Chairman of the Board, to succeed ... President and CEO, but will continue as a member of ... that he has added to the company over almost twenty ... The Board of Directors has established a Search ...
(Date:12/17/2014)... Based on the revenues gained from ... is dominated by four major players contributing to ... players include GE Healthcare (U.K.), Siemens AG (Germany), ... Full Report Copy at http://www.marketsandmarkets.com/Market-Reports/surgical-imaging-market-210534462.html , ... attributed to its strong product portfolio, which includes ...
Breaking Biology Technology:LayerBio Awarded NIH Grant to Develop a Drug Delivery Device for Cataract Surgery 2Global Refurbished Surgical Imaging Market by Product & Application is Estimated to Reach $1.25 Billion by 2019 - New Research Report by MarketsandMarkets 2Global Refurbished Surgical Imaging Market by Product & Application is Estimated to Reach $1.25 Billion by 2019 - New Research Report by MarketsandMarkets 3Global Refurbished Surgical Imaging Market by Product & Application is Estimated to Reach $1.25 Billion by 2019 - New Research Report by MarketsandMarkets 4
... in a lab dish to the steroid DHEA, exhibit a ... play a role in helping the brain produce new cells, ... online editions of the Proceedings of the National Academy of ... of scientists at the University of Wisconsin-Madison, provides some of ...
... , a data quality solutions provider, announced today ... Information Quality Suite to work with Oracle Warehouse ... intelligence integration design tool that manages data lifecycles. ... intelligence application and customize the way data quality ...
... Inc. announced Tuesday that its genetic analysis ... chromosomal analysis, opening a new market to the ... extension of the Invader technology, Madison-based Third Wave ... (ASRs) for prenatal chromosomal analysis to the University ...
Cached Biology Technology:DHEA Boosts Growth Rate of Human Neural Stem Cells 2DHEA Boosts Growth Rate of Human Neural Stem Cells 3
(Date:11/21/2014)... , Nov. 20, 2014   Atmel® Corporation ... microcontroller (MCU) and touch technology solutions, today launched the ... with the widest V cc range from ... accuracy and faster I 2 C bus communication speeds, ... EEPROM memory making them ideal for consumer, industrial, computer, ...
(Date:11/18/2014)... BETHESDA, Md. , Nov. 17, 2014 The ... European and U.S. regulatory agencies will speak and at least ... Quality Metrics Conference at the Omni Shoreham Hotel in ... is pleased once again to have significant support from the ... and Europe in our effort to ...
(Date:11/12/2014)... Nov. 12, 2014 Crossmatch™, a leading provider ... U.are.U ® fingerprint readers have been deployed throughout ... Central Mexico . The bakery chain implemented ... payroll issues caused by employees clocking in for each ... fingerprint readers, Montparnasse relied on paper timecards and a ...
Breaking Biology News(10 mins):Atmel Launches Industry's First Wide-V(cc) Low-Power Temperature Sensor Family 2Atmel Launches Industry's First Wide-V(cc) Low-Power Temperature Sensor Family 3Atmel Launches Industry's First Wide-V(cc) Low-Power Temperature Sensor Family 4FDA's Janet Woodcock, EMA's Emer Cooke Headline PDA Quality Metrics Conference 2Montparnasse Pasteleria Achieves Time and Attendance Transparency with Crossmatch U.are.U Fingerprint Readers 2
... M.D., director of the National Institute of Allergy and Infectious ... been awarded the 2007 Mary Woodard Lasker Award for Public ... health programs, in AIDS and biodefense. The award will be ... New York City. , Dr. Fauci is honored for his ...
... in conjunction with the National Evolutionary Synthesis Center, will ... 1, 2007, at the Hyatt Regency Hotel in Atlanta, ... and Populations, is part of the 2007 Professional Development ... The aim of the symposium is ...
... National Institutes of Health has awarded one of 12 ... a team based at the University of Chicago Medical ... 2006, form the core of an NIH effort to ... "transform how clinical and translational research is conducted," ultimately ...
Cached Biology News:Anthony S. Fauci awarded Lasker Award for Public Service 2Anthony S. Fauci awarded Lasker Award for Public Service 3Anthony S. Fauci awarded Lasker Award for Public Service 4AIBS to cohost symposium on evolution, disease and human health 2AIBS to cohost symposium on evolution, disease and human health 3AIBS to cohost symposium on evolution, disease and human health 4NIH awards nearly $23M to University of Chicago for translational research 2NIH awards nearly $23M to University of Chicago for translational research 3NIH awards nearly $23M to University of Chicago for translational research 4
... Agarose Gel-Digesting Preparation contains a unique β-agarose ... quantitative recovery of intact DNA and RNA ... following electrophoresis in TAE, TBE, MOPS, or ... directly in the TAE, TBE, MOPS, and ...
...
... a unique β-agarose digesting enzyme developed at ... DNA and RNA from low melting point ... TBE, MOPS, or phosphate buffers. The gel ... TBE, MOPS, and phosphate electrophoresis buffers or ...
Request Info...
Biology Products: