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Greater Amplification Specificity with New Hot Start PCR Enzyme

mplify the 115-bp gag gene fragment (Figure 2) were 1 cycle at 95C for 1 minute (Taq and Taq plus hot start antibody) or 10 minutes (SureStart Taq), followed by 40 cycles at 95C for 1 minute (denaturation); 55C for 1 minute (annealing); and 72C for 1 minute, followed by one final extension cycle of 72C for 10 minutes. The conditions used to amplify the 970-bp lambda DNA fragment (Figure 3) were 1 cycle at 93C for 1 minute (Taq and Taq plus hot start antibody), 10 minutes (SureStart Taq) or 10 to 15 minutes (competitors modified Taq preparations); 45 cycles at 93C for 1 minute (denaturation); 60C for 50 seconds (annealing); and 68C for 1 minute, followed by one final extension cycle of 68C for 10 minutes.


REFERENCES

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4. Mullis, K. (1991) PCR Methods Appl. 1: 1-4.
5. Aquila, R.T., et al. (1991) Nucl. Acids Res. 19: 3749.
6. Chou, Q., et al. (1991) Nucl. Acids Res. 20: 7.
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