Navigation Links
Greater Amplification Specificity with New Hot Start PCR Enzyme

he target from 500 and 5000 copies of HIV-1 template, nonspecific products predominated in reactions using 5, 50, and 500 copies (Figure 2). In contrast, SureStart Taq DNA polymerase produced specific amplification product from as few as five copies of DNA template, and no background was visible (Figure 2).

In Figure 3, a 970-bp fragment was amplified from 101 to 105 copies of lambda DNA in the absence or presence of 0.5 mg of denatured genomic DNA. In a complex DNA sample, multiple nonspecific products were generated, and specific product yield was reduced (Figure 3A). When compared to Taq, SureStart Taq DNA polymerase successfully amplified the desired product from as few as 101 copies of lambda DNA, even in the presence of high concentrations of extraneous denatured DNA. Moreover, SureStart Taq DNA polymerase generated far fewer nonspecific products compared to Taq DNA polymerase. Comparisons with other vendors hot start enzymes showed similar improvements in specific product yield (Figure 3B, Lanes 1 to 3) but high background was evident in two of the three hot start preparations tested.

Conclusions

Use Stratagenes SureStart Taq DNA polymerase to achieve a simple and effective method for hot start. SureStart Taq DNA polymerase improves the sensitivity of amplification reactions by reducing background and increasing yield of desired product. Compared to other vendors hot start Taq preparations, SureStart Taq provides comparable or superior detection of low-copy-number targets. SureStart Taq is inactive at ambient temp
'"/>

Source:


Page: All 1 2 3 4 5 6 7

Related biology technology :

1. Enjoy a Greater Sense of Security with PCR-Grade Water
2. prostar RT-PCR Systems for Robust High-Fidelity RNA Amplification
3. Simple Purification of DNA from Plasmid Minipreps, PCR Amplifications and Agarose Gels
4. Enhanced Amplification of Long Targets with PfuTurbo DNA Polymerase
5. Optimizing pfuturbo DNA Polymerase Amplification Reactions with Perfect Match PCR Enhancer
6. Improve Amplification Specificity with Hot Start PCR Enzyme
7. Amplification of the amoA Gene of Nitrosococcus oceani Bacteria Using Eppendorf MasterTaq
8. Amplification of Mouse cDNAs for Microarrays Using the Eppendorf MasterTaq Kit
9. Optimizing the Direct Amplification of Missing cDNA 5 Ends Using the Eppendorf Mastercycler gradient
10. Optimizing DNA Amplification Protocols using the Eppendorf Mastercycler
11. Amplification of Epstein-Barr Virus Exon C with Difficult Primers Using Eppendorf Mastercycler gradient
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Greater Amplification Specificity with New Hot Start PCR Enzyme

(Date:12/24/2014)... AUSTIN, Texas , Dec. 24, 2014 /PRNewswire/ ... company focused on gynecologic disease, announced today that ... with investors including Oracle Investment Management, Jack ... Vermillion directors. Total proceeds were $10.5 million, before ... working capital and general corporate purposes. ...
(Date:12/24/2014)... December 23, 2014 Earlier this year ... L. Sherley, director of the Adult Stem Cell Technology ... often overlooked and under appreciated unique property of adult ... Distributed Stem Cells: Misunderstood in the Past, Important for ... congress participants. He gave the address at the ...
(Date:12/24/2014)... (PRWEB) December 23, 2014 ... mercaptan such as its definition, classification, application ... product specification, manufacturing process, and product cost ... and applications. The analysis also covers upstream ... channels, industry development trend and proposals. In ...
(Date:12/24/2014)... , Dec. 23, 2014 China ... the "Company"), a leading fully integrated plasma-based biopharmaceutical ... announced that its majority-owned subsidiary, Shandong Taibang Biological ... ("GMP") certification from the China Food and Drug ... production facility. As previously disclosed in the Company,s ...
Breaking Biology Technology:Vermillion Announces Closing of Equity Financing 2Vermillion Announces Closing of Equity Financing 3Adult Stem Cell Technology Center, LLC’s Director Sherley's Address on What’s Holding Back Regenerative Medicine Featured on Omics Group Website 2Adult Stem Cell Technology Center, LLC’s Director Sherley's Address on What’s Holding Back Regenerative Medicine Featured on Omics Group Website 3Adult Stem Cell Technology Center, LLC’s Director Sherley's Address on What’s Holding Back Regenerative Medicine Featured on Omics Group Website 4Worldwide Methyl Mercaptan Market 2015-2020 Forecasts on Development & Trends Now Available at ReportsnReports.com 2China Biologic Receives GMP Certification for New Coagulation Factor Facility 2China Biologic Receives GMP Certification for New Coagulation Factor Facility 3
... LASIK Eye Surgeons directory service and information resource ... the Trusted LASIK Surgeons directory apart is a ... LASIK Surgeons in the United States are listed. ... R. Wayne Bowman, are screened based on their ...
... is to determine whether therapy after childbirth for women with ... Willebrand disease is needed and for how ... Behring is providing,a $1.2 million grant to fund a first-of-its ... for determining optimal prophylaxis,treatment for postpartum women with von Willebrand ...
... Mass., Aug. 18 Logical Therapeutics, Inc., ... medicines that treat,diseases associated with poorly controlled ... Phase 1b randomized controlled multi-dose,safety and pharmacokinetics ... The study, which was conducted from December, ...
Cached Biology Technology:Trusted LASIK Surgeons Welcomes Dallas LASIK Eye Surgery Specialist Dr. R. Wayne Bowman to its LASIK Directory 2Trusted LASIK Surgeons Welcomes Dallas LASIK Eye Surgery Specialist Dr. R. Wayne Bowman to its LASIK Directory 3Trusted LASIK Surgeons Welcomes Dallas LASIK Eye Surgery Specialist Dr. R. Wayne Bowman to its LASIK Directory 4Trusted LASIK Surgeons Welcomes Dallas LASIK Eye Surgery Specialist Dr. R. Wayne Bowman to its LASIK Directory 5Trusted LASIK Surgeons Welcomes Dallas LASIK Eye Surgery Specialist Dr. R. Wayne Bowman to its LASIK Directory 6Video: CSL Behring Provides $1.2M Grant for World's First Study of Postpartum Women with VWD, Common Bleeding Disorder 2Video: CSL Behring Provides $1.2M Grant for World's First Study of Postpartum Women with VWD, Common Bleeding Disorder 3Video: CSL Behring Provides $1.2M Grant for World's First Study of Postpartum Women with VWD, Common Bleeding Disorder 4Logical Therapeutics Inc. Announces Results of Phase 1b Clinical Study of its Naproxen Prodrug 2
(Date:12/17/2014)... , Dec. 16, 2014  Automation is fundamentally ... more evident than at international borders. Over the ... scanners have allowed veteran travelers to self process ... Control (APC) Kiosks at an increasing number of ... According to Maxine Most ...
(Date:12/11/2014)... , Dec. 09, 2014 Research and ... ( http://www.researchandmarkets.com/research/9ql3kr/biometrics_market ) has ... in India 2015-2019" report to their offering. ... this market is the adoption of multimodal biometric ... characteristic of an individual for verification and identification ...
(Date:12/10/2014)... NEW YORK , Dec. 8, 2014 You,ve ... online banking account but can,t remember your password, site key ... birthday? Who was your first grade teacher? ... launches the app that will finally put an ... PINs – 1U TM . 1U leverages a ...
Breaking Biology News(10 mins):Automated Border Control (ABC) Transforms the Global Travel Experience With More Than 2500 ABC eGates and APC Kiosks Deployed In Airports, Seaports, and Land Borders Worldwide 2Biometrics Market in India 2015-2019: Key Vendors are 3M Cogent, NEC, Safran and Suprema 2The Password is Finally Dead: Launch of 1U Mobile App Eliminates Need for All Usernames and Passwords 2The Password is Finally Dead: Launch of 1U Mobile App Eliminates Need for All Usernames and Passwords 3
... that Western University scientists call "Forrest Gump" because, like ... aren,t smart, is furthering the understanding of a key ... team at Robarts Research Institute say the mice show ... available in the brain. Boosting ACh is a ...
... Calif. , June 20, 2013 Based on ... & Sullivan recognizes geneOnyx Limited with the 2013 European ... leverages a DNA testing platform originally used for medical ... anti-aging cosmetics. geneOnyx,s test requires only a ...
... of the patients who received injections of steroids contaminated ... Compounding Center has found that some patients had fungal ... of their symptoms and that magnetic resonance imaging (MRI) ... received injections from highly contaminated lots. The ...
Cached Biology News:'Forrest Gump' mice show too much of a good thing, can be bad 2Frost & Sullivan Applauds geneOnyx for its DNA Testing Solution for the Personalized Cosmeceuticals Market 2Frost & Sullivan Applauds geneOnyx for its DNA Testing Solution for the Personalized Cosmeceuticals Market 3Frost & Sullivan Applauds geneOnyx for its DNA Testing Solution for the Personalized Cosmeceuticals Market 4Evolution of an outbreak: Complications from contaminated steroid injections 2
Values of steady state parameters for enzyme activities are determined....
... (PPAR) is a member of a ... factors that heterodimerize with retinoic acid-like ... the regulation of glucose and lipid ... of anti-diabetic agents called thiazolidinediones (TZD) ...
... This protein identification Service includes 1. ... protein spots 3. High-sensitive tryptic in-gel ... of samples for MALDI-MS 5. Acquisition ... via MALDI-MS (Bruker ultraflex TOF/TOF) 6. ...
... Preparation Reconstitution: one vial contains ... ficoll and 50 mg PVP; ... water yields a 50concentrate Special ... 1.00 g/mL (20 C) Quality ...
Biology Products: