Navigation Links
Greater Amplification Specificity with New Hot Start PCR Enzyme

sequencing of specific PCR products. Moreover, synthesis of misprimed products diverts reaction components (dNTPs, primers) away from the production of desired products, which leads to poor amplicon yield.

Various techniques have been developed to reduce nonspecific primer extension by Taq DNA polymerase at low temperatures. One method involves replacing Taq with Stratagenes Pfu DNA polymerase, which is less prone to zero-cycle artifacts due to lower activity at nonrestrictive temperatures (e.g., 8% maximum activity at 50C).1 Another method for reducing nonspecific amplification employs Stratagenes Perfect Match PCR enhancer, which decreases background by destabilizing mismatched primer-template complexes.2,3 In addition, various hot start procedures have been developed to prevent nonspecific primer extension at low temperatures. In these procedures, a critical PCR component (e.g., dNTPs, polymerase, or Mg2+) is withheld until stringent primer annealing temperatures are reached.4-7 The missing component can be added manually at the initial denaturation step4,5 or withheld by a physical wax barrier until temperatures sufficient to melt wax are achieved.6

More recently, reversibly inactivated DNA polymerases have been used to provide hot start. Heat-reversible inhibition is accomplished using neutralizing antibodies,8 oligonucleotide inhibitors,9 or enzyme modification.10 DNA polymerases reversibly inactivated by antibodies regain activity upon denaturation of the antibody (1 minute at 80C 8) durin
'"/>

Source:


Page: All 1 2 3 4 5 6 7

Related biology technology :

1. Enjoy a Greater Sense of Security with PCR-Grade Water
2. prostar RT-PCR Systems for Robust High-Fidelity RNA Amplification
3. Simple Purification of DNA from Plasmid Minipreps, PCR Amplifications and Agarose Gels
4. Enhanced Amplification of Long Targets with PfuTurbo DNA Polymerase
5. Optimizing pfuturbo DNA Polymerase Amplification Reactions with Perfect Match PCR Enhancer
6. Improve Amplification Specificity with Hot Start PCR Enzyme
7. Amplification of the amoA Gene of Nitrosococcus oceani Bacteria Using Eppendorf MasterTaq
8. Amplification of Mouse cDNAs for Microarrays Using the Eppendorf MasterTaq Kit
9. Optimizing the Direct Amplification of Missing cDNA 5 Ends Using the Eppendorf Mastercycler gradient
10. Optimizing DNA Amplification Protocols using the Eppendorf Mastercycler
11. Amplification of Epstein-Barr Virus Exon C with Difficult Primers Using Eppendorf Mastercycler gradient
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Greater Amplification Specificity with New Hot Start PCR Enzyme

(Date:5/30/2015)... May 30, 2015  Caris Life Sciences® today ... in which researchers identified biomarker changes, including key ... tumors, indicating changes in response to therapy as ... was a subset of a larger study analyzing ... the cancer. These data utilizing Caris Molecular Intelligence®, ...
(Date:5/29/2015)... CA (PRWEB) May 29, 2015 ... addressing multi-drug-resistant organisms (MDROs), announced that initial feasibility ... same-shift bacterial detection and susceptibility testing from positive ... on May 31, 2015 in New Orleans, ... class of molecular diagnostics that can quickly detect ...
(Date:5/29/2015)... 2015 Delcath Systems, Inc. (NASDAQ: ... company focused on oncology with an emphasis on ... announces that results of a large, European single-center ... with hepatic metastases with the Delcath Hepatic CHEMOSAT ... on-line abstract at the American Society of Clinical ...
(Date:5/29/2015)... 2015 Research and Markets ( http://www.researchandmarkets.com/research/7bf7ff/cytogenetics ... new report "Cytogenetics - Technologies, Markets and ... report deals with cytogenetics in a broader sense ... the chromosome structure and identify abnormalities related to ... is also referred to as molecular cytogenetics. The ...
Breaking Biology Technology:Caris Life Sciences Paired Tumor Data Identifies Key Driver Mutations and Confirms Tumor Heterogeneity in High-Grade Glioma Samples 2Caris Life Sciences Paired Tumor Data Identifies Key Driver Mutations and Confirms Tumor Heterogeneity in High-Grade Glioma Samples 3Caris Life Sciences Paired Tumor Data Identifies Key Driver Mutations and Confirms Tumor Heterogeneity in High-Grade Glioma Samples 4Data Highlighting GeneWEAVE’s Smarticles™ Technology to be Presented at the 115th General Meeting of the American Society for Microbiology (asm2015) 2Data Highlighting GeneWEAVE’s Smarticles™ Technology to be Presented at the 115th General Meeting of the American Society for Microbiology (asm2015) 3Positive Data With Delcath's CHEMOSAT Highlighted At ASCO 2015 2Positive Data With Delcath's CHEMOSAT Highlighted At ASCO 2015 3Positive Data With Delcath's CHEMOSAT Highlighted At ASCO 2015 4Positive Data With Delcath's CHEMOSAT Highlighted At ASCO 2015 5Global Cytogenetics Market Report 2015-2024 - Technologies, Markets and Companies 2Global Cytogenetics Market Report 2015-2024 - Technologies, Markets and Companies 3
... , , Zdechlik ... , , NEW YORK, ... molecular oncology diagnostics to aid in early cancer detection and ... Chief Operating Officer. As COO, Zdechlik will be responsible ...
... ... Holden to present the keynote address , ... Parsipany, NJ (PRWEB) July 22, 2009 -- Vistaar Technologies, ... finance groups, today announced the company will partner with the Professional Pricing Society (PPS), ...
... , LYNBROOK, N.Y., July 22 ... developing first in class collagenase-based products, today announced that BioSpecifics, President, ... Small-Cap Investor Conference on Wednesday, July 29, 2009, at 9:30am CT ... , A live webcast of the presentation can be ...
Cached Biology Technology:Exosome Diagnostics Appoints Former Roche Executive Greg Zdechlik as Chief Operating Officer 2Vistaar and the Professional Pricing Society Partner to Present Premier Symposium for Pricing Practitioners 2Vistaar and the Professional Pricing Society Partner to Present Premier Symposium for Pricing Practitioners 3
(Date:5/11/2015)... , May 11, 2015  Synaptics Incorporated (NASDAQ: ... interface solutions, today announced the appointment of ... Chief Financial Officer, reporting to Rick Bergman ... current Chief Financial Officer, Kathleen Bayless , ... Mr. Ali brings extensive financial management ...
(Date:5/10/2015)... GOTHENBURG, Sweden , May 11, 2015 ... touch fingerprint sensors FPC1025 and FPC1155 from the distributor World ... one of FPC,s distributors in Asia . ... the sensors will be used by smartphone manufacturers in ... is included in the communicated revenue guidance of + 1 000 ...
(Date:5/6/2015)... , May 6, 2015 LifeBEAM, a ... established helmet producer, announced today that they will expand ... the world,s first bio-sensing cycling helmet and the first ... in two new colors in order to give cyclists ... product. In addition, LifeBEAM and Lazer announced their plan ...
Breaking Biology News(10 mins):Synaptics Appoints Wajid Ali as Senior Vice President and Chief Financial Officer 2Fingerprint Cards Receives Touch Fingerprint Sensor Order of SEK 235 Million 2LifeBEAM and Lazer Announce Expanded Partnership in Bio-Sensing Sports Gear 2LifeBEAM and Lazer Announce Expanded Partnership in Bio-Sensing Sports Gear 3
... it instinctively avoids the feline or risks becoming dinner. How? ... sense of smell, provides evidence that a single gene is ... neurobiologist Thomas Bozza has shown that removing one olfactory receptor ... The gene, called TAAR4, encodes a receptor that responds to ...
... songs in tropical waters during the winter breeding season, ... times of the year. NOAA researchers have provided ... acoustic behavior on a feeding ground in the Northwest ... in the journal PLOS ONE , demonstrate the ...
... Institutet in Sweden have managed to obtain detailed images of ... into cells. Since tumours are highly dependent on the transportation ... the researchers are hoping that the study published in the ... form the basis for new strategies to fight cancer cells. ...
Cached Biology News:Cat and mouse: A single gene matters 2Cat and mouse: A single gene matters 3Researchers track singing humpback whales on a Northwest Atlantic feeding ground 2Mapping of cancer cell fuel pumps paves the way for new drugs 2
... The Model 1575 immunowash microplate washer ... microplates that have flat-, U-, or V-bottom ... of horizontal and vertical needle positions to ... washing, crosswise aspiration, and overflow washing. It ...
... compact benchtop and stackable multi-function incubator. ... a large display screen to accurately ... circulation inside to evenly distribute temperature ... easily calibrated from the microprocessor controller. ...
... specifically designed as a high tech shaker ... mass compensation allows high shaking speed even ... and, therefore, faster growth and higher cell ... when high reproducibility is critical ...
...
Biology Products: