( ...)Greater Amplification Specificity with New Hot Start PCR Enzyme

SureStart Taq DNA polymerase makes hot start simple and efficient

Jonathan Eads Frances Bai Ronda Allen Holly Hogrefe

Stratagene

SureStart Taq DNA polymerase is a modified form of Taq2000 DNA polymerase with hot start capability to increase PCR specificity and improve performance. Using SureStart Taq DNA polymerase, hot start is incorporated into PCR protocols previously optimized with Taq DNA polymerase, with little or no modification of cycling parameters or reaction conditions. SureStart Taq DNA polymerase can be used in a variety of amplification systems to improve sensitivity, specificity, and product yield.

Certain PCR enzymes exhibit significant polymerase activity at temperatures encountered during reaction setup or while ramping up to stringent primer annealing temperatures. For example, Taq DNA polymerase exhibits 2% maximum activity at 25C (room temperature) and 70% maximum activity at 50C (generally at or below the T_m of PCR primers).¹ When PCR reactants are combined at temperatures below stringent primer annealing temperatures, nonspecific primer annealing and extension can occur. Once formed, misprimed products and primer oligomers are efficiently amplified throughout remaining PCR cycles. Undesired products and primer-dimers can impair gel analysis, quantitation, and
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