Navigation Links
Gram Quantities of MAb Produced with Simple Bioreactor in Serum-Free Perfusion Culture Replacing Ascitic Fluid Production

By J. Doyle, L. Johnstone and L. Cottis
AGEN Biomedical Ltd. Queensland, Australia
http://www.agen.com.au


The CelliGen Plus immobilized-bed perfusion was investigated as a means for producing large quantities of MAb from a hybridoma, as an alternative to in vivo production. This system best represented a production facility that was easy to operate, flexible in its use, small enough to enable autoclaving and yet large enough to produce the required quantities of MAb. Three cell culture trials were performed using serum-free medium. Each run proceeded in the same manner, with MAb yields of 39.3, 33.8 and 30.3 grams. The perfusion system enabled us to scale down the size of the reactor needed, as the yields were significantly higher than that obtainable in batch fermentation which would have necessitated a bioreactor of several hundred liters.


Materials and Methods
All runs were performed in a 7.5 Liter NBS CelliGen Plus bioreactor with the basket impeller (New Brunswick Scientific, Edison, NJ). Working volume was 5 L and the bed volume was 2.6 L. Cells were inoculated at a cell density of 2 x 105 cells/mL at > 80% viability. Temperature was maintained at 37C, pH 7.20 and top dissolved oxygen (DO) at 50% of air saturation. The pH was controlled via the interactive pH-DO controller with CO2 at the initial stage and then with an 8% NaHCO3 solution when perfusion reached 2 volumes/day. Agitation rate was initiated at 80 rpm and increased throughout the run to maintain the bottom DO concentration at > 35% air saturation. The maximal rate did not exceed 150 rpm. Gas supply was via the ring sparger at a rate not exceeding 0.13 LPM and this was only increased
'"/>

Source:


Page: All 1 2

Related biology technology :

1. Simple, Sensitive, and Rapid Detection of FLAG -Tagged Fusion Proteins
2. A Simple and Rapid Technique to Purify High-Quality Midiprep DNA
3. Simple Purification of DNA from Plasmid Minipreps, PCR Amplifications and Agarose Gels
4. Simple, Sensitive Isotyping of Mouse Monoclonal Antibodies
5. Simple Isolation of RNA from Tissue and Cultured Cells
6. HSVision Molecular Beacon Detection Module Rapidly Detects Herpes Simplex Virus DNA
7. Simple and Rapid Isolation of Plant RNA Using the Eppendorf Perfect RNA, Eukaryotic, Maxi Kit
8. The Fastest, Simplest, and Most Effective Way to Remove DNA Contamination
9. Simple Approaches for Optimization of RT-PCR
10. Detection of Morphine and Morphine-Glucuronide in Saliva or Urine with a Rapid and Simple Method Without Derivatization on the Varian 500-MS
11. CHO Cell Culture Application Using a BioFlo 110 Benchtop Bioreactor
Post Your Comments:
(Date:8/21/2014)... at the Institute for Molecular Medicine Finland (FIMM), ... developed a novel "man and machine" decision support ... aid was described in PLOS One ... based on computer vision algorithms similar to those ... of only the diagnostically most relevant areas. Tablet ...
(Date:8/21/2014)... (PRWEB) August 21, 2014 ... Fertilizer Industry” is a professional and in-depth ... The report provides basic Seaweed Fertilizer information, ... industry chain structure as well as industry ... analysis, including domestic market as well as ...
(Date:8/21/2014)... 21, 2014 His Majesty Willem-Alexander, King ... The Court of His Majesty Willem-Alexander, King of the ... Grand Opening of POET-DSM Advanced Biofuels’ Project LIBERTY cellulosic ... , The King will take part in the Grand ... for 11 a.m.-12:20 p.m. and tour the plant. POET-DSM ...
(Date:8/21/2014)... The College of New Rochelle announced today ... Mid-Hudson Region to be designated as a START-UP NY ... qualified “high-technology” businesses that align with the College’s academic ... been selected for the START-UP NY program and extremely ... economic development in New York by growing business and ...
Breaking Biology Technology:A novel 'man and machine' decision support system makes malaria diagnostics more effective 2Internationals Seaweed Fertilizer Industry 2014 Development Trend Analysis in New Research Study at DeepResearchReports.com 2Internationals Seaweed Fertilizer Industry 2014 Development Trend Analysis in New Research Study at DeepResearchReports.com 3His Majesty Willem-Alexander, King of the Netherlands, to Attend Project LIBERTY opening 2His Majesty Willem-Alexander, King of the Netherlands, to Attend Project LIBERTY opening 3The College of New Rochelle Becomes First Private College in Mid-Hudson Region to Earn START-UP NY Designation 2The College of New Rochelle Becomes First Private College in Mid-Hudson Region to Earn START-UP NY Designation 3
... and Electric (MG&E) awarded Intergraph Mapping ... upgrade its existing FRAMME-based AM/FM/GIS application to G/Technology, ... scheduled for May. , ,G/Technology will provide MG&E ... environment and includes solutions designed to streamline everyday ...
... on the Midwest technology job market today, youve learned ... learned something about interviewing, thank-you letters and follow-up calls ... an older job seeker, though, you may have run ... your search: your age. Wait. Isn't age discrimination illegal? ...
... Firstlogic, Inc. announced the general availability ... Postalsoft Business Edition is designed to help organizations ... in the necessary order and format to receive ... , ,The program includes features designed to decrease ...
Cached Biology Technology:How 40-plus tech job seekers can prevent age discrimination 2How 40-plus tech job seekers can prevent age discrimination 3How 40-plus tech job seekers can prevent age discrimination 4
(Date:8/21/2014)... of the water window? It consists of radiations in ... absorbed by the water in biological tissues. New theoretical ... radiations within the water window. These could be the ... image of the biological samples or to be used ... the physical mechanism needed to efficiently generate the harmonic ...
(Date:8/21/2014)... University (ANU) team has successfully replicated one of the ... systems powered by sunlight which could manufacture hydrogen as ... sunlight. It is an exciting prospect to use them ... said Dr Kastoori Hingorani, from the ARC Centre of ... of Biology. , Hydrogen offers potential as a zero-carbon ...
(Date:8/20/2014)... Institute (HCI) at the University of Utah have identified ... BCR-ABL, the unregulated enzyme driving the blood cancer chronic ... nearly 6,000 new cases of CML will be diagnosed ... kinase inhibitors (TKIs), target BCR-ABL and are effective at ... control it in a way that allows patients to ...
Breaking Biology News(10 mins):Water window imaging opportunity 2Water and sunlight the formula for sustainable fuel 2Blueprint for next generation of chronic myeloid leukemia treatment 2
... doesn,t kill in the prostate it,s only once the ... fatal. Previous studies have shown that loss of the protein ... Cancer Center study published this week in the Journal ... switch that regulates the production of E-Cadherin: the transcription factor ...
... Biologists from the Ruhr-Universitt Bochum have explored how to ... the journal " Molecular and Cellular Neuroscience " they ... P, ensures the survival of motor neurons in culture. ... the molecule Brain Derived Neurotrophic Factor (BDNF), which was ...
... reprogram cancer cells that is, if these nucleic acids ... Cancer Center study published today in the journal Therapeutic ... into cancer cells. "There are many promising therapeutic ... cell membranes on their own, delivery to cancer cells has ...
Cached Biology News:Landmark study describes prostate cancer metastasis switch 2Vitamin P as a potential approach for the treatment of damaged motor neurons 2Cholesterol rafts deliver drugs inside cancer cells 2
Control primers and template for 5kb and 15 kb products....
The Lambda Control Kit contains lambda DNA and two 25mer primers, which can be used to amplify a 500bp fragment. The lambda DNA is purified by CsCl gradient centrifugation and organic extraction foll...
... is a derivative of Taq DNA polymerase. ... aquaticus DNA polymerase. Expressed from a gene ... Met236, bypassing the 5'-3' exonuclease domain of ... a highly active and even more heat-stable ...
Homo sapiens similar to Cytosolic acyl coenzyme A thioester hydrolase Antigen: Peptide...
Biology Products: