Navigation Links
Gq-coupled receptor assays using FLEXstation: M1 muscarinic receptor

and the mean of the negative control. The Z factor obtained for FLEXstation using the FLEXstation Calcium Assay Kit was 0.65. This indicates a large separation band between the negative and positive controls and an optimized, high quality assay.


SUMMARY
FLEXstation generates highly reproducible results for calcium mobilization assays used to monitor the activity of Gq-coupled receptors (Fig. 3). First, the EC50 values obtained for carbachol were consistent between detection methods, Fluo-3 and the FLEXstation Calcium Assay Kit. Also, assays analyzed on the same day showed less than two-fold differences, and assays analyzed on different days showed less than fourfold differences, which further demonstrates the reliable performance of FLEXstation (data not shown). Lastly, the Z factor determination indicates that FLEXstation provides an excellent tool for assay development for the model system examined. In addition, other benefits include SOFTmax PRO software that makes it simple to set up the instrument and analyze the data. Moreover, the dual monochromators provide increased flexibility for assay development or basic research purposes. In conclusion, FLEXstation provides an easy-to-use, versatile tool to help basic research and assay development laboratories reach their goals.

For more detailed information, please refer to the application note Comparison of FLIPR and FLEXstation for Calcium Mobilization Assays, available for download from Molecular Devices web site at:
www.moleculardevices.com/pages/flex_lit1.html


REFERENCES
 1. Buck, M. A., and C. M. Fraser. 1990. Biochem. Biophys. Res. Commun. 173: 666672.
2. Zhang
'"/>

Source:


Page: All 1 2 3 4

Related biology technology :

1. Gq-coupled Receptor Assays Using FLEXstation
2. Gq-coupled receptor assays: a comparison of FLEXstation and FLIPR
3. The development of a CXCR2 chemokine receptorbinding assay using the LEADseeker Multimodality Imaging System
4. The development of a melanocortin MC4 receptor binding assay using the LEADseeker Multimodality Imaging System
5. The development of a melanocortin MC5 receptor binding assay using the LEADseeker Multimodality Imaging System
6. The development of a calcitonin-gene related peptide (CGRP) receptor-binding assay using the LEADseeker Multimodality Imaging System
7. Screening for potential beta 2-adrenergic receptor antagonists using CypHer5E and IN Cell Analyzer 1000
8. The use of CypHer5 for receptor internalization studies in both a range of GPCRs and a non-GPCR
9. The use of CypHer5E and the IN Cell Analyzer 1000 for live-cell receptor internalization studies
10. Screening for β2-adrenergic receptor agonists using the pH-sensitive dye,CypHer5, and the IN Cell Analyzer 3000
11. Development of a histamine H1 receptor binding assay using the LEADseeker Multimodality Imaging System
Post Your Comments:
(Date:5/23/2013)... May 23, 2013 PuraMed BioScience ® ... marketer of over-the-counter (OTC) medicinal and healthcare products, ... on the company based on low market capitalization ... Non-Prescription, All Natural Product to Treat Migraine Headaches ... #1 Prescription Competitor , Currently Available Nationwide ...
(Date:5/23/2013)... CA (PRWEB) May 23, 2013 The ... encourage all types of inventions. They focus on learning ... noticing this community gravitate toward bringing their ideas to ... DIY Biology Movement, works on making projects using biology. ... DNA of plants to make them glow with bioluminescence ...
(Date:5/23/2013)... , May 23, 2013 VG ... strategic alliance with DAK Renewable Research to conduct ... oil yield. VG Energy is a majority owned ... VGLS). VG Energy,s exclusively licensed compound ... the oil yield in plants, possessing a wide ...
(Date:5/23/2013)... , May 23, 2013 Virobay, Inc. ... for pain has reached an important milestone, as Virobay ... VBY-036 - a selective cathepsin S inhibitor.   ... a double-blind, randomized, placebo-controlled study designed to evaluate the ... VBY-036 in healthy adults. "The initiation of ...
Breaking Biology Technology:PuraMed BioScience (PMBS) Issued Alert Based on Market Cap of $721,756 2PuraMed BioScience (PMBS) Issued Alert Based on Market Cap of $721,756 3Glowing Plant Releases Maker Kit, Enabling Anyone to Make a Glowing Plant at Home 2VG Energy Enters into a Strategic Alliance with DAK Renewable Research for LipidMax Field Trials 2VG Energy Enters into a Strategic Alliance with DAK Renewable Research for LipidMax Field Trials 3Virobay, Inc. initiates a Phase 1 Trial of VBY-036, a compound intended for the treatment of neuropathic pain 2Virobay, Inc. initiates a Phase 1 Trial of VBY-036, a compound intended for the treatment of neuropathic pain 3
... The Raymond and Ruth Perelman School ... announces the launching of a first-of-its-kind interdisciplinary center ... The Center will bring together, without institutional walls, ... diseases: establishing dedicated research support facilities, translating findings ...
... 26, 2011 Neogen Corporation (NASDAQ: NEOG ) ... which ended May 31, increased 30% from the previous year ... $0.96 per share, compared to the prior year,s $0.76 per ... 23% to $172,683,000, up from $140,509,000 in the company,s previous ...
... Fla., July 26, 2011 Dyadic International, Inc. (OTC ... it will report its financial results for the second ... Thursday, August 4, 2011 and will host a conference ... financial results. (Logo: http://photos.prnewswire.com/prnh/20110621/CL06708LOGO) Those ...
Cached Biology Technology:Penn Receives $10 Million to Create Center for Orphan Disease Research and Therapy 2Neogen Reports 30% Increase in Net Income 2Neogen Reports 30% Increase in Net Income 3Neogen Reports 30% Increase in Net Income 4Neogen Reports 30% Increase in Net Income 5
(Date:5/23/2013)... , the team reveal the interplay of brain chemicals ... the Wellcome Trust and BBSRC. , By changing ... our perceptual abilities. While these changes can affect the ... the fidelity of that response. , Lead ... you communicate with others, you can make yourself better ...
(Date:5/22/2013)... Forest Service scientists are part of "Landscapes of Resilience", ... Foundation, will examine how collaborative planning and stewardship of ... , The TKF Foundation announced today that Landscapes of ... funding. In addition to research on the role of ... the 3-year, $585,000 grant will contribute to the creation ...
(Date:5/22/2013)... working to improve durability in fuel cell powered ... have discovered links between electrode degradation processes and ... the effects of electrode degradation stressors in the ... lifetime. , The findings of the study, led ... latest in a long-term study at Burnaby-based Ballard ...
Breaking Biology News(10 mins):'Landscapes of Resilience' to study how people use nature as a source of recovery 2Tests lead to doubling of fuel cell life 2
... BioMed Central,s premier open access medical journal Genome ... its January 2009 issue. Genomic medicine is at ... of fastest growing areas of biomedical research. Our growing ... it possible to identify the genetic changes that underlie ...
... that contain instructions for making proteins make up less ... reasons, most of our genome is transcribed into RNA. ... are easier to study than humans. Researchers in the ... Laboratory (EMBL) in Heidelberg, Germany, and Wolfgang Huber at ...
... FDA has approved the first human clinical trial of ... but not only, or even primarily, because what is ... Although deep-seated disagreements about the ethics of embryo research ... focus as well on this being the first human ...
Cached Biology News:Genome Medicine: Bridging the gap between research and clinical practice 2Rewrite the textbooks: Transcription is bidirectional 2
... increasingly essential for reducing sample and ... other biochemical applications. The eight-channel nano25 ... in the industry. ,Features: , ... dispensed volumes , Automated diagnostics detect ...
...
...
Collected from sexually mature rats....
Biology Products: