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Gq-coupled receptor assays using FLEXstation: M1 muscarinic receptor

and the mean of the negative control. The Z factor obtained for FLEXstation using the FLEXstation Calcium Assay Kit was 0.65. This indicates a large separation band between the negative and positive controls and an optimized, high quality assay.


SUMMARY
FLEXstation generates highly reproducible results for calcium mobilization assays used to monitor the activity of Gq-coupled receptors (Fig. 3). First, the EC50 values obtained for carbachol were consistent between detection methods, Fluo-3 and the FLEXstation Calcium Assay Kit. Also, assays analyzed on the same day showed less than two-fold differences, and assays analyzed on different days showed less than fourfold differences, which further demonstrates the reliable performance of FLEXstation (data not shown). Lastly, the Z factor determination indicates that FLEXstation provides an excellent tool for assay development for the model system examined. In addition, other benefits include SOFTmax PRO software that makes it simple to set up the instrument and analyze the data. Moreover, the dual monochromators provide increased flexibility for assay development or basic research purposes. In conclusion, FLEXstation provides an easy-to-use, versatile tool to help basic research and assay development laboratories reach their goals.

For more detailed information, please refer to the application note Comparison of FLIPR and FLEXstation for Calcium Mobilization Assays, available for download from Molecular Devices web site at:
www.moleculardevices.com/pages/flex_lit1.html


REFERENCES
1. Buck, M. A., and C. M. Fraser. 1990. Biochem. Biophys. Res. Commun. 173: 666672.
2. Zhang
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