Navigation Links
Gq-coupled Receptor Assays Using FLEXstation

Alexander C. Zambon1, Bruce R. Conklin1, Laurence L. Brunton2,3, Paul A. Insel2,3, and Anne T. Ferguson4

1Gladstone Institute of Cardiovascular Disease, University of California, San Francisco, CA 94141-9100
Departments of Pharmacology2 and Medicine3, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093-0636
4Molecular Devices Corp., 1311 Orleans Drive, Sunnyvale, CA 94089


Introduction
Gq-coupled receptors are cell-surface proteins that signal through multiple pathways, resulting in the release of Ca2+ from intracellular storage vesicles. They are gaining widespread popularity because they are implicated in critical intra- and intercellular processes. Since abnormal regulation of Gq-coupled receptor signaling pathways can cause various diseases, these receptors are prime targets for drug therapy. For this reason, it is important to study the regulation and specificity of Gq-coupled receptor activity.

Traditionally, Gq-coupled receptor activity is monitored by measuring an increase in intracellular inositol triphosphates (IP3) and/or calcium ions (Ca2+) (Fig. 1). Inositol phosphate measurement using [3H] inositol is labor intensive and requires radioactive waste storage and removal. In comparison, the release of calcium from intracellular stores into the cytoplasm was traditionally monitored in single cells by using fluorescence microscopy image analysis and a calcium-sensitive fluorescent dye. This procedure, while allowing a view of individual cells, is laborious and time-consuming when multiple ligands or drugs are being tested.


'"/>

Source:


Page: All 1 2 3 4 5 6 7

Related biology technology :

1. Gq-coupled receptor assays using FLEXstation: M1 muscarinic receptor
2. Gq-coupled receptor assays: a comparison of FLEXstation and FLIPR
3. Antibodies for Studying NMDA Receptor Protein Expression and Synapse-Specific Immunolabeling
4. Detecting Gastrin-Releasing Peptide Receptor by in situ PCR on Archived Tissue
5. pFB-ERV: Retroviral Delivery of the Ecdysone Receptor Proteins
6. Gene-Specific Primers for NMDA Receptor Subunits
7. TKB1 Cells Identify Receptor Tyrosine Kinase Interacting Proteins
8. Quantification of the Purinergic Receptor P2X3 using ICAT and Orthogonal 2D LC-MS/MS with an Ion Trap Mass Spectrometer
9. Isolation of Low Molecular Weight Digestion Products of the Human Platelet Thromboxane A2 Receptor, Rev A
10. Performance of Ad-A-Gene EGFP-Glucocorticoid Receptor (EGFP-GCCR): an adenoviral vector gene delivery system
11. Use of FlashPlate Technology for In Vitro Measurement of [35S]-GTPγS Binding in CHO Cells Expressing the Human 5-HT1B Receptor
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: coupled Receptor Assays Using FLEXstation

(Date:6/29/2015)... ... June 30, 2015 , ... This cutting edge standard making is a madatory ... distinct peaks, the WAV-8 is the world’s first cuvette-shaped, solid-state filter that lets metrologists ... NIR range. Previous spectrophotometer calibration standards, such as those made from didymium glass were ...
(Date:6/29/2015)... June 29, 2015  AACC, a global scientific ... through laboratory medicine, is pleased to announce that ... Chemistry , has increased to 7.9 in the ... places Clinical Chemistry in the top ... significant influence of the research it publishes on ...
(Date:6/29/2015)... , June 29, 2015   Veracyte, Inc ... pioneering the field of molecular cytology, today announced that ... Group,s prestigious Top Workplaces list for the second year ... winners based solely on an annual survey of Bay ... honored that the degree to which Veracyte values our ...
(Date:6/26/2015)... 2015 /PRNewswire/ - iCo Therapeutics ("iCo" or "the Company") ... nominees listed in the management information circular dated June ... Annual Meeting of Shareholders, held earlier today, June ... following 5 nominees proposed by management were elected ... the Company,s next Annual Meeting of Shareholders or ...
Breaking Biology Technology:FireflySci Releases Spectrophotometer Calibration Standards for NIR Wavelength Accuracy Verification 2FireflySci Releases Spectrophotometer Calibration Standards for NIR Wavelength Accuracy Verification 3AACC's Journal, Clinical Chemistry, Continues to Have Significant Impact on Medical Testing, Healthcare 2AACC's Journal, Clinical Chemistry, Continues to Have Significant Impact on Medical Testing, Healthcare 3Veracyte Named a 2015 "Top Workplace" in the San Francisco Bay Area 2Veracyte Named a 2015 "Top Workplace" in the San Francisco Bay Area 3iCo Therapeutics Announces Voting & Election Results from Annual Meeting of Shareholders 2iCo Therapeutics Announces Voting & Election Results from Annual Meeting of Shareholders 3
... BETHESDA, Md., Nov. 10 Spherix Incorporated,(Nasdaq: SPEX ... its,application to transfer the listing of its common stock ... The common stock is expected to,commence trading on the ... Nasdaq Capital Market is one of three market tier ...
... Conference Call Today at 4:30 p.m. Eastern Time -, ... MDVN ) today reported on its corporate progress and ... "The third quarter represented another quarter of significant,achievement and ... partnering,agreement with Pfizer for Dimebon. This collaboration not only ...
... Rise, Net Loss Narrows Compared to Prior Year; Operating Loss ... ... Inc.,(Nasdaq: ENDO ), an innovative medical device company focused on the,development ... tumor ablation, today reported,higher revenue, a 26 percent decline in operating loss, ...
Cached Biology Technology:Spherix Transfers Stock Listing to Nasdaq Capital Market 2Medivation Reports Third Quarter 2008 Financial Results and Provides Corporate Update 2Medivation Reports Third Quarter 2008 Financial Results and Provides Corporate Update 3Medivation Reports Third Quarter 2008 Financial Results and Provides Corporate Update 4Medivation Reports Third Quarter 2008 Financial Results and Provides Corporate Update 5Medivation Reports Third Quarter 2008 Financial Results and Provides Corporate Update 6Medivation Reports Third Quarter 2008 Financial Results and Provides Corporate Update 7Medivation Reports Third Quarter 2008 Financial Results and Provides Corporate Update 8Endocare Reports Financial Results for Third Quarter 2008 2Endocare Reports Financial Results for Third Quarter 2008 3Endocare Reports Financial Results for Third Quarter 2008 4Endocare Reports Financial Results for Third Quarter 2008 5Endocare Reports Financial Results for Third Quarter 2008 6Endocare Reports Financial Results for Third Quarter 2008 7Endocare Reports Financial Results for Third Quarter 2008 8Endocare Reports Financial Results for Third Quarter 2008 9Endocare Reports Financial Results for Third Quarter 2008 10Endocare Reports Financial Results for Third Quarter 2008 11
(Date:6/17/2015)... , June 17, 2015  Crossmatch™, a ... announced that its U.are.U ® 4500 fingerprint ... Eateries, Inc., a San ... chain, to increase security, improve accountability and reduce ... enable instant, non-repudiable identity confirmation for employee sign ...
(Date:6/16/2015)... , June 16, 2015 Fingerprint ... previously communicated that it will amount to at least 340 ... Due to the rapid increase in market growth and orders ... previously communicated guidance that revenue for 2015 will exceed 1,500 ... amount to approximately 2,200 MSEK. Due to receipt ...
(Date:6/12/2015)... 2015  Securus Technologies, a leading provider of ... safety, investigation, corrections and monitoring, announced the United ... capture, analyze and identify participants of a video ... This system can: , Identify ... subsequent sign-on process is actually the person participating ...
Breaking Biology News(10 mins):Togo's Eateries Improve Security and Accountability with Crossmatch Fingerprint Readers 2Togo's Eateries Improve Security and Accountability with Crossmatch Fingerprint Readers 3Fingerprint Cards Raises the Revenue Guidance for Q2 2015 and for the Full Year 2015 2Fingerprint Cards Raises the Revenue Guidance for Q2 2015 and for the Full Year 2015 3Securus Announces Patent Issued for Authorized Presence Verification During Video Visitation 2
... New studies released today underscore the potential impact ... of aging, and learning. The research focused on the ... presented at Neuroscience 2013, the annual meeting of the ... emerging news about brain science and health. The ...
... believed that microorganisms that produce methane swim toward the hydrogen ... too hard to prove in the lab. Montana State ... verify it for the first time, said Matthew Fields, an ... a new paper describing the find. In the process, ...
... tyrosine kinases are a large family of cell-surface ... signals, including insulin, growth factors such as epidermal ... and molecules involved in neuronal guidance. Ligand binding ... leading to recruitment of enzymes and adapter proteins ...
Cached Biology News:Research reveals roles for exercise and diet in aging, depression 2Montana State team overcomes challenges, proves that microbes swim to hydrogen gas 2
... is a new product number, ... product number. If showing no ... the old Sigma-Aldrich number (C7671) ... assistance. Mfr Desig: Corning ...
ABgene® supplies a 94kDa ultra-pure recombinant thermostable DNA polymerase obtained by high level expression of the Taq DNA polymerase gene in E. coli....
Rabbit Anti-Mouse Ym1 Polyclonal Antibody,...
ABgene® supplies a 94kDa ultra-pure recombinant thermostable DNA polymerase obtained by high level expression of the Taq DNA polymerase gene in E. coli....
Biology Products: