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Got Small RNA?

g of an siRNA targeting GAPDH mRNA or the Silencer Negative Control #1 siRNA (NC, Ambion) in 400 ml of siRNA Electroporation Buffer (Ambion). Total RNA and protein were isolated with the mirVana PARIS Kit 48 hours after electoporation. Total RNA (1 g) was used to detect the indicated RNA species by Northern blot or by solution hybridization assay with the mirVana miRNA Detection Kit (Ambion). RNA probes were prepared by in vitro transcription (mRNA probes: MAXIscript Kit, Ambion) or by 5' end labeling (miRNA and siRNA probes: mirVana Probe & Marker Kit, Ambion). Western blots were performed with 15 g of total protein and antibodies specific for GAPDH or Ku p70 proteins (Ambion).


Complete Kits

The mirVana Isolation Kits include sufficient reagents to perform 40 total RNA isolations or 20 enrichment/depletion procedures. Each purification can accommodate 100-107 cells or 1-100 mg of tissue for the mirVana PARIS Kit, up to 1 g of tissue for the mirVana miRNA Isolation Kit.


Scientific Contributors
Lesslie Beauchamp, Dmitriy Ovcharenko, Emmanuel Labourier Ambion, Inc.


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Ordering Information
Cat# Product Name Size 1556 mirVana PARIS Kit up to 40 purifications
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Related biology technology :

1. Fast Isolation of Total RNA from Small Numbers of Cells
2. Detecting Attomole Amounts of Small RNA
3. New Tools for Small RNA Analysis
4. Rapidly Prepare Probes and Markers for Small RNA Analysis
5. Ultra-Fast Purification of Small Nucleic Acids
6. Small-Molecule Quantitation Using Automated Single-Shot Averaging MALDI-TOF Mass Spectrometry
7. Maintaining High Sensitivity when Working with Small-Volume Samples
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