Navigation Links
Got Small RNA?

Total RNA or fractions depleted or enriched in small RNA were isolated from 1x106 HeLa cells with the mirVana PARIS Kit as per protocol. A 1 g aliquot of each fraction was resolved on a 1.2% denaturing glyoxal agarose gel or a 15% denaturing acrylamide gel and stained with ethidium bromide. The same fractions were also analyzed with an RNA 6000 NanoChip on the Agilent 2100 bioanalyzer.


One Sample mRNA +Small RNA + Protein

In addition to efficient isolation of RNA of all sizes and the optional small RNA enrichment procedure, the mirVana PARIS Kit was also designed to quantitatively recover total proteins from the same sample. This is especially important in experiments where the expression level of both the active miRNA or siRNA and the corresponding target protein need to be measured. Figure 3 demonstrates the effectiveness of the mirVana PARIS Kit for preparing samples for this type of experiment. Here, GAPDH knockdown was triggered by electroporation of a GAPDH-specific siRNA in a primary human cell line (normal human umbilical vein endothelial cells or HUVEC). A reduction of GAPDH expression was observed both at the mRNA and protein levels in these cells, but not in HUVEC cells electroporated with a control Negative Control #1 siRNA sequence (NC). No variation of expression levels was detected for control mRNA (-actin), small RNA (miR-16), and protein (Ku p70).

Figure 3. Using the mirVana PARIS Kit to Analyze RNAi. HUVEC cells (1.5x106) were electroporated with 10
'"/>

Source:


Page: All 1 2 3 4 5

Related biology technology :

1. Fast Isolation of Total RNA from Small Numbers of Cells
2. Detecting Attomole Amounts of Small RNA
3. New Tools for Small RNA Analysis
4. Rapidly Prepare Probes and Markers for Small RNA Analysis
5. Ultra-Fast Purification of Small Nucleic Acids
6. Small-Molecule Quantitation Using Automated Single-Shot Averaging MALDI-TOF Mass Spectrometry
7. Maintaining High Sensitivity when Working with Small-Volume Samples
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Got Small RNA

(Date:1/23/2015)... 2015 Biofertilizers stimulate microbial activities in ... and phosphorous through the activities in the soil or ... due to the stress on reducing environmental pollution and ... report defines and segments the bio fertilizers market with ...
(Date:1/23/2015)... January 23, 2015 Portal Solutions , ... workplaces on Microsoft Office 365 and SharePoint platforms , ... Award program for being one of the region’s fastest-growing mid-sized ... employee growth. , “To be recognized a second year ...
(Date:1/22/2015)... Md. (PRWEB) January 22, 2015 ... its new UV-1280 multipurpose UV-visible spectrophotometer. Offering wavelength ... high-quality instrument is ideal for applications in a ... life science. , The user-friendly UV-1280 enables intuitive ...
(Date:1/22/2015)... 22, 2015 GEA Niro Soavi the ... laboratory homogenizer, the PandaPLUS 2000, which is ideal for ... and cell disruption . This compact laboratory homogenizer ... juices, liquid food, food additives and ingredients as well ...
Breaking Biology Technology:Biofertilizers Market Expected to Reach $1,649.7 Million by 2019 - New Report By MarketsandMarkets 2Biofertilizers Market Expected to Reach $1,649.7 Million by 2019 - New Report By MarketsandMarkets 3Biofertilizers Market Expected to Reach $1,649.7 Million by 2019 - New Report By MarketsandMarkets 4Washington SmartCEO Announces Portal Solutions as 2015 Future 50 Award Winner Second Year in a Row 2Washington SmartCEO Announces Portal Solutions as 2015 Future 50 Award Winner Second Year in a Row 3Shimadzu’s New Monitored Single-Beam UV-Vis Spectrophotometer Offers Comprehensive Measurement Options in a Compact Body 2GEA Niro Soavi Announces the PandaPLUS 2000 Homogenizer for Processing New Applications for Nanoemulsions and Cell Disruption 2
... Mass., May 13 Interleukin Genetics,Inc. (Amex: ILI ... will present at the Rodman and Renshaw 5th Annual ... a.m. CEST (4:00 a.m.,EDT). The conference will be held ... A live audio webcast of the presentation will be ...
... science researchers, ST. LOUIS, May 13 ... LLC, a leading market,research company for the life ... ( http://www.sigma-aldrich.com/catalog ) is considered,by scientists to be ... catalog team represents the long-term commitment of some ...
... LAKE FOREST, Ill., May 13 Hospira, Inc.,(NYSE: ... medication delivery,company, announced today that Barbara L. Bowles, ... directors. Bowles brings strong investment,management and finance expertise ... companies and equity investment advisory,firms. The addition of ...
Cached Biology Technology:Interleukin Genetics to Present at Rodman & Renshaw 5th Annual Global Healthcare Conference 2Sigma-Aldrich Recognized by BioInformatics, LLC as Having the Most Useful and Easiest to Use Online and Print Catalogs 2Sigma-Aldrich Recognized by BioInformatics, LLC as Having the Most Useful and Easiest to Use Online and Print Catalogs 3Hospira Expands Board of Directors 2
(Date:12/22/2014)... 22, 2014  The 2014 Holiday Season may be the ... Intelligence reports that the long anticipated floodgates for consumer ... demand for smart phones, tablets, and wearable mobile devices ... 2.5 billion users with nearly 4.8 billion biometric devices ...
(Date:12/19/2014)... 18, 2014 Research and Markets ( http://www.researchandmarkets.com/research/86ncd6/micro_market ) ... North America Perimeter Security Systems Market" report to their ... The North American perimeter security market is estimated to grow ... the U.S. market holds a larger share in this market, ...
(Date:12/19/2014)...   LaunchKey , the first decentralized mobile authentication and ... Things era, today announced the close of $3 million ... by Metamorphic Ventures with participation from ENIAC Ventures, Crosslink ... LaunchKey has raised $4 million to date, and will ...
Breaking Biology News(10 mins):First Season of Holiday Shopping with Mobile Biometric Payments Wraps Up With a Present for Biometrics Industry: A Rosy Forecast for More Than 2 Billion Users of 4.8 Billion Mobile Biometric Devices by 2020 2Micro Market Monitor: North America Perimeter Security Systems Market (Intrusion Detection Sensor, Video Surveillance, Communication/Alarm and Notification, Access Control System) 2Micro Market Monitor: North America Perimeter Security Systems Market (Intrusion Detection Sensor, Video Surveillance, Communication/Alarm and Notification, Access Control System) 3LaunchKey Raises $3 Million in Additional Funding Led by Metamorphic Ventures 2LaunchKey Raises $3 Million in Additional Funding Led by Metamorphic Ventures 3
... farming has introduced the equivalent of five Connecticut Rivers into ... adding more carbon dioxide annually into its waters, according to ... Louisiana State universities. Its like the discovery of ... belt, said Pete Raymond , lead author of the ...
... percent of all modern potato varieties planted today are ... the lowlands of south-central Chile. How Chilean germplasm came ... been the subject of a long, contentious debate among ... that Chilean potatoes were the first to be planted ...
... 1st and January 15th issues of Biophysical Journal, ... online. Topics of interest include ciliates, force, spasmoneme, ... Volume 94, Issue 1, January 1, 2008 ... an ultra-fast biological spring Arpita Upadhyaya, Massachusetts ...
Cached Biology News:Agriculture is changing the chemistry of the Mississippi River 2Using DNA, scientists hunt for the roots of the modern potato 2Media highlights in the January issues of Biophysical Journal 2Media highlights in the January issues of Biophysical Journal 3
... Radiochemical Purity is >95%NEN Radiolabeled Ligands\n\nReceptor-related research ... availability of new radiolabeled ligands selected to ... a wide range of products and services ... over 400 state-of-the-art radioligands. If you do ...
... TNT Coupled Reticulocyte Lysate Systems(a,b,c,d) offer ... vitro translations: a single-tube, coupled transcription/translation ... simplify the process and reduce the ... translation results. Standard rabbit reticulocyte lysate ...
... Identical to the DH-2000, except that it ... 190-1700 nm wavelength range and comes with a ... up to 5 Hz) The DH-2000 ... spectrum of deuterium and tungsten halogen light sources ...
... These handy dispensers save space and ... and other garb. They are the ... parts used in labs, processing and ... charges and the particles they attract. ...
Biology Products: