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Got Small RNA?

bion.com/catalog/CatNum.php?1556">mirVana PARIS Kit retains the small RNA fraction.

Figure 1. Differential Recovery of Small RNAs. Total RNA was isolated from 1x106 HeLa cells with the indicated kit as per protocol. Total RNA (1 g) was resolved on a 15% denaturing acrylamide gel and stained with ethidium bromide or analyzed by solution hybridization assay with the mirVana miRNA Detection Kit (Ambion) and a probe specific for miR-16 prepared by in vitro transcription with the mirVana Probe Construction Kit (Ambion). The gel was exposed for 6 h at -80C.


You Can Also Enrich the Small RNA Fraction

The mirVana Isolation Kit also provides reagents and a procedure to enrich the population of RNAs that are 200 bases and smaller. Small RNA enrichment results in lower background and enhanced sensitivity of small RNA detection by solution hybridization, Northern analysis, and other methods, as compared to the same assay using total RNA. A representative enrichment/depletion example with the mirVana PARIS Kit is presented in Figure 2. The depleted and enriched fractions, or total RNA, were purified from the same number of HeLa cells. Analysis of each fraction on a denaturing agarose gel, a denaturing acrylamide gel or the Agilent 2100 bioanalyzer showed that 28S, 18S, and 5.8 rRNAs were quantitatively recovered in the fraction depleted in small RNA. In contrast, RNAs smaller than 200 nt such as 5S RNA and tRNA were significantly enriched in the complementary "enriched" fraction when compared to the total RNA sample.

Figure 2. Small RNA Enrichment Procedure.
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Related biology technology :

1. Fast Isolation of Total RNA from Small Numbers of Cells
2. Detecting Attomole Amounts of Small RNA
3. New Tools for Small RNA Analysis
4. Rapidly Prepare Probes and Markers for Small RNA Analysis
5. Ultra-Fast Purification of Small Nucleic Acids
6. Small-Molecule Quantitation Using Automated Single-Shot Averaging MALDI-TOF Mass Spectrometry
7. Maintaining High Sensitivity when Working with Small-Volume Samples
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