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Got Small RNA?

Ensure the Presence of Small RNA, Including miRNAs, in Your RNA Sample

Isolate both large and small RNA efficiently
Enrich for small RNA (10-200 nt)
Purify RNA quickly (~30 minutes)


Not All RNA Isolation Procedures Purify Small RNAs

Ambion scientists have developed a line of mirVana RNA Isolation Kits for the preparation of miRNA and other small RNA species. Other RNA purification procedures rely on organic extraction followed by alcohol precipitation or adsorption of nucleic acids on a silicate matrix, such as a glass fiber filter (GFF). Unfortunately, alcohol precipitation is time consuming and sometimes inefficient at recovering small RNAs. Furthermore, adsorption to solid supports (e.g. RNeasy Kit from Qiagen) usually results in loss of most of the small RNA fraction, including small ribosomal RNA (5S rRNA), transfer RNA (tRNA), microRNA (miRNA), or small interfering RNA (siRNA).


Efficient Recovery of Small RNAs

The mirVana miRNA Isolation Kits, which employ organic extraction followed by purification on a GFF, use specialized binding and wash solutions that are optimized to prevent loss of the small RNA fraction. The fast and efficient GFF-based method isolates total RNA ranging in size from kilobases down to 10-mers. Figure 1 demonstrates that RNA isolated by the mirVana miRNA Isolation Kit or the

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