Navigation Links
Got Small RNA?

Ensure the Presence of Small RNA, Including miRNAs, in Your RNA Sample

Isolate both large and small RNA efficiently
Enrich for small RNA (10-200 nt)
Purify RNA quickly (~30 minutes)


Not All RNA Isolation Procedures Purify Small RNAs

Ambion scientists have developed a line of mirVana RNA Isolation Kits for the preparation of miRNA and other small RNA species. Other RNA purification procedures rely on organic extraction followed by alcohol precipitation or adsorption of nucleic acids on a silicate matrix, such as a glass fiber filter (GFF). Unfortunately, alcohol precipitation is time consuming and sometimes inefficient at recovering small RNAs. Furthermore, adsorption to solid supports (e.g. RNeasy Kit from Qiagen) usually results in loss of most of the small RNA fraction, including small ribosomal RNA (5S rRNA), transfer RNA (tRNA), microRNA (miRNA), or small interfering RNA (siRNA).


Efficient Recovery of Small RNAs

The mirVana miRNA Isolation Kits, which employ organic extraction followed by purification on a GFF, use specialized binding and wash solutions that are optimized to prevent loss of the small RNA fraction. The fast and efficient GFF-based method isolates total RNA ranging in size from kilobases down to 10-mers. Figure 1 demonstrates that RNA isolated by the mirVana miRNA Isolation Kit or the

Source:


Page: All 1 2 3 4 5

Related biology technology :

1. Fast Isolation of Total RNA from Small Numbers of Cells
2. Detecting Attomole Amounts of Small RNA
3. New Tools for Small RNA Analysis
4. Rapidly Prepare Probes and Markers for Small RNA Analysis
5. Ultra-Fast Purification of Small Nucleic Acids
6. Small-Molecule Quantitation Using Automated Single-Shot Averaging MALDI-TOF Mass Spectrometry
7. Maintaining High Sensitivity when Working with Small-Volume Samples
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Got Small RNA

(Date:8/1/2014)... RURO, Inc., Laboratory Information Management System ... the opening of its first international office. Located ... in Shanghai, PRC, RURO Shanghai’s doors are open. ... Mr. Shen Gang, the former Technical Director of ... RURO’s has installed FreezerPro-based solutions in more than ...
(Date:8/1/2014)... 2014 The  PROPEL Center  of the  iBIO ... rate of early-stage life sciences companies in ... 15 grants to help Illinois ...   in Chicago .   AdvaMed ... North America , bringing more than 1,000 ...
(Date:8/1/2014)... BCC Research ( http://www.bccresearch.com ) reveals in its ... MARKETS , that the global market RNA interference (RNAi) ... $38.8 billion by 2018, with a five-year compound annual ... the fastest moving segment of the market, is moving ... RNAi therapeutics has shown tremendous growth and has moved ...
(Date:7/31/2014)... CA (PRWEB) July 31, 2014 ... consumers, are increasingly searching for food alternatives that ... nutritional benefits. The food ingredient market has numerous ... players such as Vital Force Technology (VFT). ... more than 4,000 energetic patterns, all tested to ...
Breaking Biology Technology:RURO Opens Office in Shanghai, China 2iBIO Institute's PROPEL Center Awards Grants for AdvaMed 2014 to Early-Stage Life Sciences Companies 2iBIO Institute's PROPEL Center Awards Grants for AdvaMed 2014 to Early-Stage Life Sciences Companies 3iBIO Institute's PROPEL Center Awards Grants for AdvaMed 2014 to Early-Stage Life Sciences Companies 4Global Market for RNAi Drug Delivery Technologies to Reach $38.8 Billion in 2018; Aptamer Delivery Segment Surging at 58.2% CAGR 2Global Market for RNAi Drug Delivery Technologies to Reach $38.8 Billion in 2018; Aptamer Delivery Segment Surging at 58.2% CAGR 3Functional Ingredient Manufacturer – Vital Force Technology Infuses Raw Materials & Ingredients with Subtle Energy Formula 2
... 18 Derma Sciences, Inc.,(OTC Bulletin Board: DSCI), a ... increased its direct-sales staff significantly, based on a,number of ... The,company, which had only two direct salespersons in the ... to 21 by 2009. The increase is a significant ...
... ACEL ) today announced that it has ... Protein Laboratories LLC (SPL) for the,commercial production of ... currently being evaluated as a treatment for,unresectable malignant ... "SPL has been involved in the production ...
... International Group, Ltd.,(Bloomberg: BIG SP, Company or Biosensors), ... (CE) Mark approval for its,BioMatrix(R) drug-eluting stent system, ... and the countries in Asia and Latin America,that ... system,developed internally by the Company, consists of a ...
Cached Biology Technology:Derma Sciences Expands Direct Sales Force for Advanced Wound Care, Looks to Double Again in 2008 2Derma Sciences Expands Direct Sales Force for Advanced Wound Care, Looks to Double Again in 2008 3Alfacell Announces ONCONASE(R) Purchase and Supply Agreement with Scientific Protein Laboratories 2Alfacell Announces ONCONASE(R) Purchase and Supply Agreement with Scientific Protein Laboratories 3Biosensors Receives CE Mark Approval for Its BioMatrix(R) Drug-Eluting Coronary Stent System 2Biosensors Receives CE Mark Approval for Its BioMatrix(R) Drug-Eluting Coronary Stent System 3Biosensors Receives CE Mark Approval for Its BioMatrix(R) Drug-Eluting Coronary Stent System 4
(Date:7/31/2014)... by the National Science Foundation (NSF), counters a ... atmospheric warming, indicating instead that certain Arctic lakes ... the atmosphere. , The study, published this ... on thermokarst lakes, which occur as permafrost thaws ... fresh water, converting what was previously frozen land ...
(Date:7/31/2014)... known that biomass burning burning forests to ... , slash-and-burn agriculture and wildfires figures into ... until the release of a new study by ... Z. Jacobson, the degree of that contribution had ... in a paper published July 30 in the ...
(Date:7/31/2014)... hazard lurks beneath many of the roughly 156,000 ... hazard is corrosion in parts of underground gas ... and contamination of groundwater, a source of drinking ... states have reported many rapidly corroding gas storage ... are generally associated with use of gasoline-ethanol blends ...
Breaking Biology News(10 mins):Certain Arctic lakes store more greenhouse gases than they release 2Stanford professor finds that wildfires and other burns play bigger role in climate change 2Stanford professor finds that wildfires and other burns play bigger role in climate change 3Stanford professor finds that wildfires and other burns play bigger role in climate change 4NIST corrosion lab tests suggest need for underground gas tank retrofits 2
... Chalfie of Columbia University , Nobel Laureate Eric Wieschaus ... Scripps Research Institute, ASCB public information chairman Rex Chisholm ... informal, good-natured, and intimate program that will use a ... targeted to young researchers. Sponsored by ASCB,s PIC, the ...
... landmark collection of papers that together provide a ... and organizations in sub-Saharan Africa addressing neglected health ... creative scientific and business solutions. The first-of-its ... entrepreneurs, institutes and firms in Africa creating innovative, ...
... the first time, scientists have created functioning human intestinal ... In a study posted online Dec. 12 by ... say their findings will open the door to unprecedented ... process is also a significant step toward generating intestinal ...
Cached Biology News:Studies detail triumphs, troubles of African innovators creating products for local health needs 2Studies detail triumphs, troubles of African innovators creating products for local health needs 3Studies detail triumphs, troubles of African innovators creating products for local health needs 4Studies detail triumphs, troubles of African innovators creating products for local health needs 5Studies detail triumphs, troubles of African innovators creating products for local health needs 6Studies detail triumphs, troubles of African innovators creating products for local health needs 7Studies detail triumphs, troubles of African innovators creating products for local health needs 8Studies detail triumphs, troubles of African innovators creating products for local health needs 9Studies detail triumphs, troubles of African innovators creating products for local health needs 10Studies detail triumphs, troubles of African innovators creating products for local health needs 11Studies detail triumphs, troubles of African innovators creating products for local health needs 12Studies detail triumphs, troubles of African innovators creating products for local health needs 13Studies detail triumphs, troubles of African innovators creating products for local health needs 14Studies detail triumphs, troubles of African innovators creating products for local health needs 15Studies detail triumphs, troubles of African innovators creating products for local health needs 16Studies detail triumphs, troubles of African innovators creating products for local health needs 17Studies detail triumphs, troubles of African innovators creating products for local health needs 18Studies detail triumphs, troubles of African innovators creating products for local health needs 19Stem cells turned into complex, functioning intestinal tissue in lab 2Stem cells turned into complex, functioning intestinal tissue in lab 3
AedestaTM Animal Organ/Tissue Perfusion Soln....
RAT ANTI MOUSE JAM-2 Immunogen: Recombinant soluble JAM-2...
casein kinase 2, alpha 1 polypeptide, transcript variant 2, mRNA (cDNA clone MGC:15232 IMAGE:4121882), complete cds...
... Spots, Oligo Spots and complementary RNA Spikes ... experiments. Use for glass array printing and ... reverse transcription, cDNA labeling, hybridization stringency and ... Control PCR Spots and Oligo Spots, and ...
Biology Products: