( Minimum Analytical Instru...)Glutamine in Urine or Blood Plasma
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Glutamine in Urine or Blood Plasma
Minimum Analytical Instrument Requirement:
Adept HPLC System 1, isocratic
UV/Visible HPLC detector
Typical Method:
Column: Nucleosil SB, 10, 250 x 4.6mm
Mobile Phase: 0.1M KH2PO4 pH 4.0
Flow Rate: 0.8 mL/minute
Detector: UV at 210nm
Peak Identification
1. L-Glutamine
2. Pyrrolidone carboxylic acid
3. Glutamic acid
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